The N-Terminal Tandem Repeat Region of Human Prion Protein Reduces Copper: Role of Tryptophan Residues

2000 ◽  
Vol 269 (2) ◽  
pp. 491-495 ◽  
Author(s):  
Francisca H. Ruiz ◽  
Eduardo Silva ◽  
Nibaldo C. Inestrosa
Keyword(s):  
Prion Protein ◽  
Tandem Repeat ◽  
Repeat Region ◽  
Tryptophan Residues ◽  
Tandem Repeat Region ◽  
Human Prion Protein

Copper(ii) coordination outside the tandem repeat region of an unstructured domain of chicken prion protein

2009 ◽  
Vol 5 (5) ◽  
pp. 497 ◽  
Author(s):  
Ewa Gralka ◽  
Daniela Valensin ◽  
Karolina Gajda ◽  
Dimitri Bacco ◽  
Łukasz Szyrwiel ◽  
...  
Keyword(s):  
Prion Protein ◽  
Tandem Repeat ◽  
Repeat Region ◽  
Tandem Repeat Region

scholarly journals OC125, M11 and OV197 Epitopes Are Not Uniformly Distributed in the Tandem-Repeat Region of CA125 and Require the Entire SEA Domain

2013 ◽  
Vol 34 (4) ◽  
pp. 257-267 ◽  
Author(s):  
Alessandro Bressan ◽  
Francesca Bozzo ◽  
Carlo Alberto Maggi ◽  
Monica Binaschi
Keyword(s):  
Ovarian Cancer ◽  
Monoclonal Antibodies ◽  
Western Blot ◽  
Tandem Repeat ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Human Cancer ◽  
Ovarian Cancer Cells ◽  
Repeat Region ◽  
Tandem Repeat Region

The human cancer antigen 125 (CA125) is over-expressed in epithelial ovarian cancer cells and it plays a role in the pathogenesis of ovarian cancer. This protein presents a repeat region containing up to sixty tandem repeat units. The anti-CA125 monoclonal antibodies have been previously classified into three groups: two major families, the OC125-like antibodies and M11-like antibodies, and a third group, the OV197-like antibodies. A model in which a single repeat unit contains all the epitopes for these antibodies has been also proposed, even if their exact position is still undetermined. In the present work, the affinities of the monoclonal antibodies, representative of the three families, have been investigated for different CA125-recombinant repeats through Western blot analysis. Different patterns of antibody recognition for the recombinant repeats show that CA125 epitopes are not uniformly distributed in the tandem repeat region of the protein. The minimal region for the recognition of these antibodies has been also individuated in the SEA domain through the subcloning of deleted sequences of the highly recognized repeat-25 (R-25), their expression as recombinant fragments inE. coliand Western blot analysis. Obtained data have been further confirmed by ELISA using the entire R-25 as coating antigen.

Production of Novel Recombinant Single-Domain Antibodies against Tandem Repeat Region of MUC1 Mucin

2004 ◽  
Vol 23 (3) ◽  
pp. 151-159 ◽  
Author(s):  
F. Rahbarizadeh ◽  
M.J. Rasaee ◽  
M. Forouzandeh Moghadam ◽  
A.A. Allameh ◽  
E. Sadroddiny
Keyword(s):  
Tandem Repeat ◽  
Single Domain ◽  
Repeat Region ◽  
Muc1 Mucin ◽  
Tandem Repeat Region ◽  
Single Domain Antibodies

Production of Monoclonal Antibody, PR81, Recognizing the Tandem Repeat Region of MUC1 Mucin

2003 ◽  
Vol 22 (3) ◽  
pp. 153-158 ◽  
Author(s):  
M. Paknejad ◽  
M.J. Rasaee ◽  
F. Karami Tehrani ◽  
S. Kashanian ◽  
M.A. Mohagheghi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Tandem Repeat ◽  
Repeat Region ◽  
Muc1 Mucin ◽  
Tandem Repeat Region

scholarly journals Molecular Cloning of Human MUC3 cDNA Reveals a Novel 59 Amino Acid Tandem Repeat Region

1997 ◽  
Vol 238 (1) ◽  
pp. 143-148 ◽  
Author(s):  
B.Jan-Willem Van Klinken ◽  
Tanja C. Van Dijken ◽  
Esmee Oussoren ◽  
Hans A. Büller ◽  
Jan Dekker ◽  
...  
Keyword(s):  
Amino Acid ◽  
Molecular Cloning ◽  
Tandem Repeat ◽  
Repeat Region ◽  
Tandem Repeat Region

Polymorphic tandem repeat region in interleukin-1α intron 6

1993 ◽  
Vol 91 (1) ◽  
pp. 85-86 ◽  
Author(s):  
Sabine Bailly ◽  
Francesco S. di Giovine ◽  
Gordon W. Duff
Keyword(s):  
Tandem Repeat ◽  
Repeat Region ◽  
Tandem Repeat Region ◽  
Interleukin 1Α

Spectroscopic and Electronic Structure Studies of Copper(II) Binding to His111 in the Human Prion Protein Fragment 106−115: Evaluating the Role of Protons and Methionine Residues

2011 ◽  
Vol 50 (5) ◽  
pp. 1956-1972 ◽  
Author(s):  
Lina Rivillas-Acevedo ◽  
Rafael Grande-Aztatzi ◽  
Italia Lomelí ◽  
Javier E. García ◽  
Erika Barrios ◽  
...  
Keyword(s):  
Electronic Structure ◽  
Prion Protein ◽  
Protein Fragment ◽  
Human Prion Protein ◽  
Methionine Residues
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