Enhanced Expression of Cellular Prion Protein Gene by Insulin or Nerve Growth Factor in Immortalized Mouse Neuronal Precursor Cell Lines

2000 ◽  
Vol 268 (3) ◽  
pp. 763-766 ◽  
Author(s):  
Chieko Kuwahara ◽  
Atsutaka Kubosaki ◽  
Takuya Nishimura ◽  
Yukiko Nasu ◽  
Yuko Nakamura ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Lines ◽  
Protein Gene ◽  
Precursor Cell ◽  
Cellular Prion Protein ◽  
Prion Protein Gene ◽  
Neuronal Precursor ◽  
Enhanced Expression ◽  
Neuronal Precursor Cell

ENHANCED EXPRESSION OF CARDIAC NERVE GROWTH FACTOR AND NERVE SPROUTING MARKERS IN RATS FOLLOWING GASTRIC PERFORATION

Shock ◽  
2010 ◽  
Vol 33 (2) ◽  
pp. 170-178 ◽  
Author(s):  
Ming-Shian Tsai ◽  
Shiu-Dong Chung ◽  
Jin-Tung Liang ◽  
Ya-Hui Ko ◽  
Wen-Ming Hsu ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Gastric Perforation ◽  
Nerve Growth ◽  
Cardiac Nerve ◽  
Nerve Sprouting ◽  
Enhanced Expression

scholarly journals Prion Protein Gene-Deficient Cell Lines: Powerful Tools for Prion Biology

2007 ◽  
Vol 51 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Akikazu Sakudo ◽  
Takashi Onodera ◽  
Kazuyoshi Ikuta
Keyword(s):  
Prion Protein ◽  
Cell Lines ◽  
Protein Gene ◽  
Prion Protein Gene ◽  
Deficient Cell

scholarly journals Regulation of Cell Growth

1987 ◽  
Vol 80 (9) ◽  
pp. 591-593
Author(s):  
A J Barrett
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Growth ◽  
Cell Lines ◽  
Growth Regulation ◽  
Platelet Derived Growth Factor ◽  
Haemopoietic Stem Cells

At this meeting of the RSM's Section of Pathology, the regulation of haemopoietic stem cells and growth factors regulating various cell lines were described, and the role of oncogenes, platelet-derived growth factor and nerve growth factor in growth regulation was discussed.

Autocrine nerve growth factor is essential for cell survival and viral maturation in HHV-8–infected primary effusion lymphoma cells

Blood ◽  
2000 ◽  
Vol 95 (9) ◽  
pp. 2905-2912 ◽  
Author(s):  
Francesca Pica ◽  
Antonio Volpi ◽  
Annalucia Serafino ◽  
Marzia Fraschetti ◽  
Ornella Franzese ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
B Cell ◽  
Cell Survival ◽  
Cell Lines ◽  
Primary Effusion Lymphoma ◽  
Human Herpesvirus ◽  
Lytic Cycle ◽  
Nerve Growth ◽  
Barr Virus

High levels of nerve growth factor (NGF) are found in sera from individuals infected with human herpesvirus 8 (HHV-8). BC-1 and BCBL-1 cells are primary effusion lymphoma–derived B-cell lines; BC-1 cells are infected by HHV-8 and the Epstein-Barr virus (EBV), and BCBL-1 cells are infected only by HHV-8. Both cells express NGF receptors and produce NGF, whereas RAMOS cells (a B-cell line that is negative for HHV-8 and EBV) express NGF receptors but do not produce detectable NGF. Neutralization of endogenous NGF results in cell growth inhibition and apoptosis in BCBL-1 cells and, to a minor extent, in BC-1 cells. When the HHV-8 lytic cycle is induced in BCBL-1 cells by tetradecanoyl phorbol acetate (TPA), an initial reduction of endogenous NGF production is observed, and many cells undergo apoptosis. However, at 48 hours, TPA-treated cells produce significantly more NGF than untreated controls, and a subsequent recovery of cell viability is observed. Consistent with this finding, the addition of exogenous NGF or anti-NGF antibodies to TPA-treated cells reduces or increases, respectively, the rate of apoptosis in response to TPA. Finally, electron microscopy of TPA-treated BCBL-1 cells shows that the addition of exogenous NGF increases the number of cells producing and releasing complete virions as compared with the controls (25% versus 5%). On the contrary, NGF neutralization leads to the production of defective viral progeny in about 2% of cells. These data indicate that NGF is essential for both cell survival and virus maturation in HHV-8–infected cell lines.

scholarly journals ELF a β-spectrin is a neuronal precursor cell marker in developing mammalian brain; structure and organization of the elf/β-G spectrin gene

Oncogene ◽  
2002 ◽  
Vol 21 (34) ◽  
pp. 5255-5267 ◽  
Author(s):  
Yi Tang ◽  
Varalakshmi Katuri ◽  
Sohail Iqbal ◽  
Tina Narayan ◽  
Zhili Wang ◽  
...  
Keyword(s):  
Brain Structure ◽  
Precursor Cell ◽  
Mammalian Brain ◽  
Cell Marker ◽  
Neuronal Precursor ◽  
Neuronal Precursor Cell

scholarly journals Processing and secretion of nerve growth factor: expression in mammalian cells with a vaccinia virus vector.

1988 ◽  
Vol 8 (6) ◽  
pp. 2456-2464 ◽  
Author(s):  
R H Edwards ◽  
M J Selby ◽  
W C Mobley ◽  
S L Weinrich ◽  
D E Hruby ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cyclic Amp ◽  
Vaccinia Virus ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Signal Peptide ◽  
Virus Vector ◽  
Mammalian Cell Lines ◽  
Vaccinia Virus Vector

To study posttranslational mechanisms for the control of nerve growth factor (NGF), we used a recombinant vaccinia virus vector to independently express the two major NGF transcripts in a variety of mammalian cell lines. The two major transcripts contain NGF (12.5 kilodaltons [kDa]) at the C-terminus and differ by alternative splicing of an N-terminal exon, so that the large precursor (34 kDa) had 67 amino acids upstream of an internal signal peptide and the smaller precursor (27 kDa) had this signal peptide at its N-terminus. In L929 cells, expression of either NGF transcript with the vaccinia virus vector gave rise to an apparently identical intracellular 35-kDa glycosylated precursor formed by cleavage of the primary gene product after the signal peptide. These cells also secreted biologically active NGF. To determine whether NGF processing is restricted by cell type, we infected a variety of mammalian cell lines with both recombinant viruses; all accumulated the same 35-kDa precursor and secreted NGF. Thus, many types of cells have the machinery to process and secrete NGF. However, NGF accumulated intracellularly (presumably in secretory granules) in cells with a regulated pathway of secretion (e.g., AtT-20 and HIT cells). In these cells, a membrane-permeable cyclic AMP analog, 8-bromo-cyclic AMP, stimulated NGF secretion. This suggests a mechanism for the regulation of NGF levels in which specific secretagogues, e.g., neurotransmitters, control NGF secretion.

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