Interactions between Cancer and Bone Marrow Cells Induce Osteoclast Differentiation Factor Expression and Osteoclast-like Cell Formation in Vitro

2000 ◽  
Vol 267 (2) ◽  
pp. 632-637 ◽  
Author(s):  
Noriko Chikatsu ◽  
Yasuhiro Takeuchi ◽  
Yasuhiro Tamura ◽  
Seiji Fukumoto ◽  
Kazuki Yano ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Osteoclast Differentiation ◽  
Cell Formation ◽  
Differentiation Factor ◽  
Factor Expression ◽  
Osteoclast Differentiation Factor ◽  
Marrow Cells

scholarly journals Crif1 Promotes Osteoporosis in Mice after Radiation

2019 ◽  
Author(s):  
Lixin Xiang ◽  
Li Chen ◽  
Yang Xiang ◽  
Fengjie Li ◽  
Xiaomei Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Resorption ◽  
Bone Loss ◽  
Bone Marrow Cells ◽  
Current Knowledge ◽  
Osteoclast Differentiation ◽  
Protein Docking ◽  
Rankl Expression ◽  
Marrow Cells

AbstractRadiation induces rapid bone loss and enhances bone resorption and RANKL expression. RANKL provides the crucial signal to induce osteoclast differentiation and plays an important role in bone resorption. However, the mechanisms of radiation-induced osteoporosis are not fully understood. Here, we show that Crif1 expression increases in bone marrow cells after radiation. Conditional Crif1 deletion in bone marrow cells causes decreases in RANKL expression and the RANKL/OPG ratio, and relieves bone loss after radiation in mice. We further demonstrated in vitro that Crif1 promotes RANKL secretion via the cAMP/PKA pathway. Moreover, protein-protein docking screening identified five compounds as Crif1 inhibitors; these compounds dramatically suppressed RANKL secretion and CREB phosphorylation when cells were exposed to forskolin. This study enriches current knowledge of the pathogenesis of osteoporosis and provides insights into potential therapeutic strategies for osteoporosis treatment.

Trehalose suppresses osteoclast differentiation in ovariectomized mice: Correlation with decreased in vitro interleukin-6 production by bone marrow cells

2000 ◽  
Vol 20 (10) ◽  
pp. 1485-1491 ◽  
Author(s):  
Chiyo Yoshizane ◽  
Norie Arai ◽  
Chikako Arai ◽  
Mayuko Yamamoto ◽  
Yasushi Nishizaki ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Interleukin 6 ◽  
Bone Marrow Cells ◽  
Osteoclast Differentiation ◽  
Ovariectomized Mice ◽  
Marrow Cells

Osteoclast-like cell formation in vitro from calcium-deficient mouse bone marrow cells

Bone ◽  
1995 ◽  
Vol 17 (6) ◽  
pp. 582
Author(s):  
J.S. Ko ◽  
J.C. Park ◽  
S.J. Kang ◽  
D.S. Lim ◽  
H.M. Kim
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Cell Formation ◽  
Mouse Bone Marrow ◽  
Deficient Mouse ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

In Vitro Osteoclast Differentiation of Bone Marrow Cells on Hydroxyapatite/Collagen Self-Organized Bone-Like Nanocomposite Disk

2008 ◽  
Vol 396-398 ◽  
pp. 449-452 ◽  
Author(s):  
Masanori Kikuchi ◽  
Atsushi Irie
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Control Cell ◽  
Osteoclast Differentiation ◽  
Mouse Bone Marrow ◽  
Self Organized ◽  
Osteoclastic Differentiation ◽  
Cells Cultured ◽  
Marrow Cells

Osteoclast differentiation of bone marrow cells on hydroxyapatite/collagen self-organized bone-like nanocomposite (HAp/Col) disk in vitro was evaluated by coculture of mouse bone marrow cells with mouse osteoblasts with or without addition of osteoclastic inducers, 1,25-Dihydroxyvitamin D3 and 1 µM prostaglandin E2. Dentine slice and tissue culture polystyrene were used as controls. Good osteoclastic differentiation at day 7 were observed among the bone marrow cells cultured on the HAp /Col disk and controls with osteoclastic inducers. On the contrary, osteoclastic differentiation was observed only for marrow cells cultured on the HAp/Col disk. Nano- and micro- structures as well as chemical and mechanical properties have a potential to control cell differentiation.

scholarly journals Interleukin-3 is significantly more effective than other colony- stimulating factors in long-term maintenance of human bone marrow- derived colony-forming cells in vitro

Blood ◽  
1989 ◽  
Vol 73 (7) ◽  
pp. 1836-1841 ◽  
Author(s):  
M Kobayashi ◽  
BH Van Leeuwen ◽  
S Elsbury ◽  
ME Martinson ◽  
IG Young ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cultured Cells ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Interleukin 3 ◽  
Gm Csf ◽  
Factor G ◽  
Marrow Cells

Abstract Human bone marrow cells cultured for 21 days in the presence of recombinant human interleukin-3 (IL-3) produced up to 28 times more colony-forming cells (CFC) than could be obtained from cultures stimulated with granulocyte colony stimulating factor (G-CSF) or granulocyte-macrophage CSF (GM-CSF). IL-3-cultured cells retained a multipotent response to IL-3 in colony assays but were restricted to formation of granulocyte colonies in G-CSF and granulocyte or macrophage colonies in GM-CSF. Culture of bone marrow cells in IL-3 also led to accumulation of large numbers of eosinophils and basophils. These data contrast with the effects of G-CSF, GM-CSF, and IL-3 in seven-day cultures. Here both GM-CSF and IL-3 amplified total CFC that had similar multipotential colony-forming capability in either factor. G-CSF, on the other hand, depleted IL-3-responsive colony-forming cells dramatically, apparently by causing these cells to mature into granulocytes. The data suggest that a large proportion of IL-3- responsive cells in human bone marrow express receptors for G-CSF and can respond to this factor, the majority becoming neutrophils. Furthermore, the CFC maintained for 21 days in IL-3 may be a functionally distinct population from that produced after seven days culture of bone marrow cells in either IL-3 or GM-CSF.

scholarly journals Antioxidant Fusion Protein SOD1-Tat Increases the Engraftment Efficiency of Total Bone Marrow Cells in Irradiated Mice

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3395
Author(s):  
Ting Bei ◽  
Xusong Cao ◽  
Yun Liu ◽  
Jinmei Li ◽  
Haihua Luo ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Fusion Protein ◽  
Bone Marrow Cells ◽  
Standard Procedure ◽  
Severe Infection ◽  
Copper Zinc Superoxide Dismutase ◽  
Donor Cells ◽  
Total Bone Marrow ◽  
Marrow Cells

Total body irradiation is a standard procedure of bone marrow transplantation (BMT) which causes a rapid increase in reactive oxygen species (ROS) in the bone marrow microenvironment during BMT. The increase in ROS reduces the engraftment ability of donor cells, thereby affecting the bone marrow recovery of recipients after BMT. In the early weeks following transplantation, recipients are at high risk of severe infection due to weakened hematopoiesis. Thus, it is imperative to improve engraftment capacity and accelerate bone marrow recovery in BMT recipients. In this study, we constructed recombinant copper/zinc superoxide dismutase 1 (SOD1) fused with the cell-penetrating peptide (CPP), the trans-activator of transcription (Tat), and showed that this fusion protein has penetrating ability and antioxidant activity in both RAW264.7 cells and bone marrow cells in vitro. Furthermore, irradiated mice transplanted with SOD1-Tat-treated total bone marrow donor cells showed an increase in total bone marrow engraftment capacity two weeks after transplantation. This study explored an innovative method for enhancing engraftment efficiency and highlights the potential of CPP-SOD1 in ROS manipulation during BMT.

In vitro proliferative advantage of bone marrow cells with tetrasomy 8 in Ewing sarcoma

1996 ◽  
Vol 90 (2) ◽  
pp. 176-178 ◽  
Author(s):  
Luba Trakhtenbrot ◽  
Yoram Neumann ◽  
Matilda Mandel ◽  
Amos Toren ◽  
Nelly Gipsh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Ewing Sarcoma ◽  
Bone Marrow Cells ◽  
Tetrasomy 8 ◽  
Proliferative Advantage ◽  
Marrow Cells
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