The Regulation of Activity of Main Mevalonic Acid Pathway Enzymes: Farnesyl Diphosphate Synthase, 3-Hydroxy-3-methylglutaryl-CoA Reductase, and Squalene Synthase in Yeast Saccharomyces cerevisiae

2000 ◽  
Vol 267 (1) ◽  
pp. 473-477 ◽  
Author(s):  
Anna Szkopińska ◽  
Ewa Świeżewska ◽  
Francis Karst
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mevalonic Acid ◽  
Squalene Synthase ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Yeast Saccharomyces Cerevisiae ◽  
Mevalonic Acid Pathway ◽  
Regulation Of Activity ◽  
Acid Pathway ◽  
Coa Reductase

scholarly journals Functional relationships between the Saccharomyces cerevisiae cis-prenyltransferases required for dolichol biosynthesis.

2005 ◽  
Vol 52 (1) ◽  
pp. 221-232 ◽  
Author(s):  
Kariona Grabińska ◽  
Grazyna Sosińska ◽  
Jacek Orłowski ◽  
Ewa Swiezewska ◽  
Thierry Berges ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Activity ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Gene Encoding ◽  
Yeast Saccharomyces Cerevisiae ◽  
Functional Relationships ◽  
Dolichyl Phosphate ◽  
Preferential Synthesis

In the yeast Saccharomyces cerevisiae the RER2 and SRT1 genes encode Rer2 and Srt1 proteins with cis-prenyltransferase (cis-PT-ase) activity. Both cis-PT-ases utilize farnesyl diphosphate (FPP) as a starter for polyprenyl diphosphate (dolichol backbone) formation. The products of the Rer2 and Srt1 proteins consist of 14-17 and 18-23 isoprene units, respectively. In this work we demonstrate that deletion or overexpression of SRT1 up-regulates the activity of Rer2p and dolichol content. However, upon overexpression of SRT1, preferential synthesis of longer-chain dolichols and a decrease in the amount of the shorter species are observed. Furthermore, overexpression of the ERG20 gene (encoding farnesyl diphosphate synthase, Erg20p) induces transcription of SRT1 mRNA and increases the levels of mRNA for RER2 and DPM1 (dolichyl phosphate mannose synthase, Dpm1p). Subsequently the enzymatic activity of Rer2p and dolichol content are also increased. However, the amount of Dpm1p or its enzymatic activity remain unchanged.

scholarly journals Inhibition of Orbivirus Replication by Fluvastatin and Identification of the Key Elements of the Mevalonate Pathway Involved

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1437
Author(s):  
Fauziah Mohd Jaafar ◽  
Baptiste Monsion ◽  
Mourad Belhouchet ◽  
Peter P. C. Mertens ◽  
Houssam Attoui
Keyword(s):  
Mevalonate Pathway ◽  
African Swine Fever Virus ◽  
Plaque Assay ◽  
African Swine Fever ◽  
Mevalonic Acid ◽  
Antiviral Effect ◽  
Structural Protein ◽  
Mevalonic Acid Pathway ◽  
Acid Pathway ◽  
Coa Reductase

Statin derivatives can inhibit the replication of a range of viruses, including hepatitis C virus (HCV, Hepacivirus), dengue virus (Flavivirus), African swine fever virus (Asfarviridae) and poliovirus (Picornaviridae). We assess the antiviral effect of fluvastatin in cells infected with orbiviruses (bluetongue virus (BTV) and Great Island virus (GIV)). The synthesis of orbivirus outer-capsid protein VP2 (detected by confocal immunofluorescence imaging) was used to assess levels of virus replication, showing a reduction in fluvastatin-treated cells. A reduction in virus titres of ~1.7 log (98%) in fluvastatin-treated cells was detected by a plaque assay. We have previously identified a fourth non-structural protein (NS4) of BTV and GIV, showing that it interacts with lipid droplets in infected cells. Fluvastatin, which inhibits 3-hydroxy 3-methyl glutaryl CoA reductase in the mevalonic acid pathway, disrupts these NS4 interactions. These findings highlight the role of the lipid pathways in orbivirus replication and suggest a greater role for the membrane-enveloped orbivirus particles than previously recognised. Chemical intermediates of the mevalonic acid pathway were used to assess their potential to rescue orbivirus replication. Pre-treatment of IFNAR(−/−) mice with fluvastatin promoted their survival upon challenge with live BTV, although only limited protection was observed.

scholarly journals The effects of statins on the mevalonic acid pathway in recombinant yeast strains expressing human HMG-CoA reductase

2013 ◽  
Vol 13 (1) ◽  
pp. 68 ◽  
Author(s):  
Agata Maciejak ◽  
Agata Leszczynska ◽  
Ilona Warchol ◽  
Monika Gora ◽  
Joanna Kaminska ◽  
...  
Keyword(s):  
Mevalonic Acid ◽  
Recombinant Yeast ◽  
Hmg Coa Reductase ◽  
Yeast Strains ◽  
Mevalonic Acid Pathway ◽  
Acid Pathway ◽  
Coa Reductase ◽  
Recombinant Yeast Strains

scholarly journals Microbial biotransformation as a tool for drug development based on natural products from mevalonic acid pathway: A review

2015 ◽  
Vol 6 (1) ◽  
pp. 17-33 ◽  
Author(s):  
Mohamed-Elamir F. Hegazy ◽  
Tarik A. Mohamed ◽  
Abdelsamed I. ElShamy ◽  
Abou-El-Hamd H. Mohamed ◽  
Usama A. Mahalel ◽  
...  
Keyword(s):  
Natural Products ◽  
Drug Development ◽  
Mevalonic Acid ◽  
Mevalonic Acid Pathway ◽  
Acid Pathway

scholarly journals Increasing the intracellular isoprenoid pool in Saccharomyces cerevisiae by structural fine-tuning of a bifunctional farnesyl diphosphate synthase

2017 ◽  
Vol 17 (4) ◽  
Author(s):  
Sebastián Rubat ◽  
Ignacio Varas ◽  
Romina Sepúlveda ◽  
Daniel Almonacid ◽  
Fernando González-Nilo ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Farnesyl Diphosphate ◽  
Fine Tuning ◽  
Farnesyl Diphosphate Synthase

scholarly journals Peroxisomal Localization of Arabidopsis Isopentenyl Diphosphate Isomerases Suggests That Part of the Plant Isoprenoid Mevalonic Acid Pathway Is Compartmentalized to Peroxisomes

2008 ◽  
Vol 148 (3) ◽  
pp. 1219-1228 ◽  
Author(s):  
Maya Sapir-Mir ◽  
Anahit Mett ◽  
Eduard Belausov ◽  
Shira Tal-Meshulam ◽  
Ahuva Frydman ◽  
...  
Keyword(s):  
Mevalonic Acid ◽  
Isopentenyl Diphosphate ◽  
Mevalonic Acid Pathway ◽  
Acid Pathway
Sign in / Sign up

Export Citation Format

Share Document