Lens Epithelium-Derived Growth Factor: Effects on Growth and Survival of Lens Epithelial Cells, Keratinocytes, and Fibroblasts

2000 ◽  
Vol 267 (1) ◽  
pp. 373-381 ◽  
Author(s):  
Dhirendra P. Singh ◽  
Nobuyuki Ohguro ◽  
Takanobu Kikuchi ◽  
Toshiharu Sueno ◽  
Venkat N. Reddy ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Lens Epithelium ◽  
Lens Epithelial Cells ◽  
Growth And Survival

Transforming growth factor-β2-mediated mesenchymal transition in lens epithelial cells is repressed in the absence of RAGE

2021 ◽  
Vol 478 (12) ◽  
pp. 2285-2296
Author(s):  
Mi-Hyun Nam ◽  
Mina B. Pantcheva ◽  
Johanna Rankenberg ◽  
Ram H. Nagaraj
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Knockout Mice ◽  
Transforming Growth Factor ◽  
Smooth Muscle Actin ◽  
Lens Epithelial Cells ◽  
Wild Type ◽  
Mesenchymal Transition ◽  
Smad Signaling ◽  
Transforming Growth Factor Β2

Transforming growth factor-β2 (TGFβ2)-mediated epithelial to mesenchymal transition (EMT) in lens epithelial cells (LECs) has been implicated in fibrosis associated with secondary cataracts. In this study, we investigated whether the receptor for advanced glycation end products (RAGE) plays a role in TGFβ2-mediated EMT in LECs. Unlike in the LECs from wild-type mice, TGFβ2 failed to elicit an EMT response in LECs from RAGE knockout mice. The lack of RAGE also diminished TGFβ2-mediated Smad signaling. In addition, treatment with TGFβ2 increased IL-6 levels in LECs from wild-type mice but not in those from RAGE knockout mice. Treatment of human LECs with the RAGE inhibitor FPS-ZM1 reduced TGFβ2-mediated Smad signaling and the EMT response. Unlike that in wild-type lenses, the removal of fiber cell tissue in RAGE knockout lenses did not result in elevated levels of α-smooth muscle actin (α-SMA), fibronectin (FN), and integrin β1 in capsule-adherent LECs. Taken together, these results suggest that TGFβ2 signaling is intricately linked to RAGE. Targeting RAGE could be explored as a therapeutic strategy against secondary cataracts.

Antibodies to Lens Epithelium-Derived Growth Factor (LEDGF) Kill Epithelial Cells of Whole Lenses in Organ Culture

1999 ◽  
Vol 69 (1) ◽  
pp. 139-142 ◽  
Author(s):  
MASAHIKO AYAKI ◽  
TOSHIHARU SUENO ◽  
DHIRENDRA P. SINGH ◽  
LEO T. CHYLACK, JR ◽  
TOSHIMICHI SHINOHARA
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Organ Culture ◽  
Lens Epithelium

Up-regulation of Fibroblast Growth Factor Receptor-1 in Lens Epithelial Cells Paralleled by Growth Stimulation

1998 ◽  
Vol 67 (5) ◽  
pp. 611-616 ◽  
Author(s):  
TOHRU MARUNOUCHI ◽  
HIROMI HOSOYA ◽  
TOSHIE MORIOKU ◽  
TAKAYUKI MORI ◽  
YOSHINAO MAJIMA ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Growth Stimulation ◽  
Lens Epithelial Cells ◽  
Fibroblast Growth
Sign in / Sign up

Export Citation Format

Share Document