Molecular Cloning and Functional Characterization of the Promoter Region of the Human Uncoupling Protein-2 Gene

1999 ◽  
Vol 265 (2) ◽  
pp. 326-334 ◽  
Author(s):  
Naxin Tu ◽  
Hongmei Chen ◽  
Ulrike Winnikes ◽  
Irmtraud Reinert ◽  
Gabriele Marmann ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Promoter Region ◽  
Uncoupling Protein ◽  
Functional Characterization ◽  
Uncoupling Protein 2

scholarly journals Porins from plants. Molecular cloning and functional characterization of two new members of the porin family.

1994 ◽  
Vol 269 (41) ◽  
pp. 25754-25760
Author(s):  
K Fischer ◽  
A Weber ◽  
S Brink ◽  
B Arbinger ◽  
D Schünemann ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
New Members

Molecular cloning and functional characterization of CvLCYE, a key enzyme in lutein synthesis pathway in Chlorella vulgaris

2021 ◽  
Vol 55 ◽  
pp. 102246
Author(s):  
Sulin Lou ◽  
Xin Lin ◽  
Chenglong Liu ◽  
Muhammad Anwar ◽  
Hui Li ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Chlorella Vulgaris ◽  
Functional Characterization ◽  
Key Enzyme

scholarly journals Molecular cloning, heterologous expression, and functional characterization of a cellulolytic enzyme (Cel PRII) from buffalo rumen metagenome

3 Biotech ◽  
2017 ◽  
Vol 7 (4) ◽  
Author(s):  
Ravi K. Shah ◽  
Amrutlal K. Patel ◽  
Deepti M. Davla ◽  
Ishan K. Parikh ◽  
Ramalingam B. Subramanian ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Functional Characterization ◽  
Cellulolytic Enzyme ◽  
Rumen Metagenome ◽  
Buffalo Rumen

Cloning and functional characterization of an uncoupling protein homolog in hummingbirds

2001 ◽  
Vol 5 (3) ◽  
pp. 137-145 ◽  
Author(s):  
CLAUDIA R. VIANNA ◽  
THILO HAGEN ◽  
CHEN-YU ZHANG ◽  
ERIC BACHMAN ◽  
OLIVIER BOSS ◽  
...  
Keyword(s):  
Uncoupling Protein ◽  
Expression System ◽  
Functional Characterization ◽  
Pectoral Muscle ◽  
Mitochondrial Fraction ◽  
Mrna Levels ◽  
Coding Region ◽  
Reading Frame ◽  
Rapid Amplification

The cDNA of an uncoupling protein (UCP) homolog has been cloned from the swallow-tailed hummingbird, Eupetomena macroura. The hummingbird uncoupling protein (HmUCP) cDNA was amplified from pectoral muscle (flight muscle) using RT-PCR and primers for conserved domains of various known UCP homologs. The rapid amplification of cDNA ends (RACE) method was used to complete the cloning of the 5′ and 3′ ends of the open reading frame. The HmUCP coding region contains 915 nucleotides, and the deduced protein sequence consists of 304 amino acids, being ∼72, 70, and 55% identical to human UCP3, UCP2, and UCP1, respectively. The uncoupling activity of this novel protein was characterized in yeast. In this expression system, the 12CA5-tagged HmUCP fusion protein was detected by Western blot in the enriched mitochondrial fraction. Similarly to rat UCP1, HmUCP decreased the mitochondrial membrane potential as measured in whole yeast by uptake of the fluorescent potential-sensitive dye 3′,3-dihexyloxacarbocyanine iodide. The HmUCP mRNA is primarily expressed in skeletal muscle, but high levels can also be detected in heart and liver, as assessed by Northern blot analysis. Lowering the room’s temperature to 12–14°C triggered the cycle torpor/rewarming, typical of hummingbirds. Both in the pectoral muscle and heart, HmUCP mRNA levels were 1.5- to 3.4-fold higher during torpor. In conclusion, this is the first report of an UCP homolog in birds. The data indicate that HmUCP has the potential to function as an UCP and could play a thermogenic role during rewarming.

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