Relationship between mRNA Levels Quantified by Reverse Transcription-Competitive PCR and Metabolic Activity of CYP3A4 and CYP2E1 in Human Liver

1999 ◽  
Vol 262 (2) ◽  
pp. 499-503 ◽  
Author(s):  
Akihiko Sumida ◽  
Kayoko Kinoshita ◽  
Tsuyoshi Fukuda ◽  
Hideyasu Matsuda ◽  
Isamu Yamamoto ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Reverse Transcription ◽  
Human Liver ◽  
Mrna Levels ◽  
Competitive Pcr

scholarly journals IL-1α, IL-1β, IL-6, and TNF-α Steady-State mRNA Levels Analyzed by Reverse Transcription-Competitive PCR in Bone Marrow of Gonadectomized Mice

1998 ◽  
Vol 13 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Rutger L. Van Bezooijen ◽  
Hetty C. M. Farih-Sips ◽  
Socrates E. Papapoulos ◽  
Clemens W. G. M. Löwik
Keyword(s):  
Bone Marrow ◽  
Steady State ◽  
Reverse Transcription ◽  
Mrna Levels ◽  
Competitive Pcr ◽  
Tnf Α

scholarly journals The usefulness of competitive PCR: airway gene expression of IL-5, IL-4, IL-4δ2, IL-2, and IFNγ in asthma

Thorax ◽  
2001 ◽  
Vol 56 (7) ◽  
pp. 541-548
Author(s):  
E M Glare ◽  
M Divjak ◽  
M J Bailey ◽  
E H Walters
Keyword(s):  
Gene Expression ◽  
Inhaled Corticosteroids ◽  
In Situ Hybridisation ◽  
Housekeeping Gene ◽  
Cross Sectional Study ◽  
Mrna Levels ◽  
Competitive Pcr ◽  
Cross Sectional ◽  
Steroid Use ◽  
Biopsy Specimens

BACKGROUNDAsthma has been described as an eosinophilic bronchitis driven by interleukin(IL)-4 and IL-5. The quantification of cytokine mRNA levels in airway samples has been confounded by housekeeping gene expression which differs between and within asthmatics and controls.METHODSThe usefulness of competitive reverse transcriptase-polymerase chain reaction (RT-PCR) that is independent of housekeeping gene expression for quantitating the mRNA for interferon (IFN)γ, IL-2, IL-5, IL-4 and its receptor antagonist encoding splicing variant IL-4δ2 was determined in a cross sectional study of 45 normal control subjects and 111 with asthma.RESULTSAtopic controls and atopic asthmatic subjects expressed more IL-5 than non-atopic controls (p<0.02) in bronchoalveolar lavage (BAL) cells, but not in biopsy specimens. IL-5 mRNA expression in BAL cells from asthmatic subjects using inhaled corticosteroids (ICS) was significantly lower than those not receiving ICS (p=0.04). IL-2 mRNA levels differed with steroid use in biopsy specimens but not in BAL cells. IFNγ, IL-4, and IL-4δ2 mRNA levels did not differ between any groups and were not affected by steroid use. IL-4 and IL-4δ2 mRNA levels were positively correlated (p<0.0001), suggesting coordinated transcription.CONCLUSIONSWhile the signal differentiation of competitive PCR in asthma may rival that of in situ hybridisation and immunohistochemistry, the method is expensive and wasteful of material.

scholarly journals Attenuation of Aggregatibacter actinomycetemcomitans virulence using curcumin-decorated nanophytosomes-mediated photo-sonoantimicrobial chemotherapy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maryam Pourhajibagher ◽  
Abbas Bahador
Keyword(s):  
Cell Viability ◽  
Metabolic Activity ◽  
Antimicrobial Chemotherapy ◽  
Drug Loading ◽  
Entrapment Efficiency ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Antimicrobial Activities ◽  
Ultrasonic Waves ◽  
Blue Laser ◽  
Mrna Levels

AbstractThis study aimed to focus on the simultaneous use of antimicrobial photodynamic therapy (aPDT) and sonodynamic antimicrobial chemotherapy (SACT), which is called photo-sonodynamic antimicrobial chemotherapy (PSACT) to attenuate the virulence of Aggregatibacter actinomycetemcomitans. Following the synthesis of Curcumin-decorated nanophytosomes (Cur-NPhs) as a novel photo-sonosensitizer, its particle size, polydispersity, ζ-potential surface morphology, physical stability, drug release, and entrapment efficiency were determined. In the Cur-NPhs-PSACT, the antimicrobial activities of Cur-NPhs against A. actinomycetemcomitans were investigated using cell viability, biofilm killing/degradation, metabolic activity, expression of quorum-sensing-associated qseB and qseC genes, and biofilm-associated rcpA gene under blue laser irradiation plus ultrasonic waves. Characterization tests showed the presence of a sphere-shaped vesicle and the self-closed structure of Cur-NPhs, resulting in a high drug-loading content and encapsulation efficiency. However, the antimicrobial effect of Cur-NPhs-PSACT was dose-dependent, PSACT using the high concentrations of Cur-NPhs (50 × 10–4 g/L) showed significant reductions (P < 0.05) in cell viability (13.6 log10 CFU/mL), biofilm killing/degradation (65%), metabolic activity (89.6%,), and mRNA levels of virulence determinant genes (qseB; 9.8-fold, qseC; 10.2-fold, and recA; 10.2-fold). This study concludes that the Cur-NPhs-PSACT had antimicrobial activities against A. actinomycetemcomitans by downregulating the expression of virulence genes, and may attenuate this bacterium that decreases periodontal disease severity in patients.

scholarly journals Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA

BioTechniques ◽  
1998 ◽  
Vol 24 (4) ◽  
pp. 652-658 ◽  
Author(s):  
L.L. Hall ◽  
G.R. Bicknell ◽  
L. Primrose ◽  
J.H. Pringle ◽  
J.A. Shaw ◽  
...  
Keyword(s):  
Mrna Levels ◽  
Competitive Pcr ◽  
Rt Pcr

Quantification of G protein mRNA using reverse transcription and competitive PCR with a colorimetric microplate assay

1998 ◽  
Vol 12 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Rupa Mokkapatti ◽  
Richard A Conn ◽  
Joseph A Carcillo ◽  
Marjorie Romkes ◽  
Edwin K Jackson
Keyword(s):  
G Protein ◽  
Reverse Transcription ◽  
Microplate Assay ◽  
Competitive Pcr
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