DNA Methylation Analysis Using Bisulfite Treatment and PCR–Single-Strand Conformation Polymorphism in Colorectal Cancer Showing Microsatellite Instability

1999 ◽  
Vol 262 (3) ◽  
pp. 671-676 ◽  
Author(s):  
Masato Maekawa ◽  
Kokichi Sugano ◽  
Hidefumi Kashiwabara ◽  
Mineko Ushiama ◽  
Shin Fujita ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Microsatellite Instability ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Methylation Analysis ◽  
Bisulfite Treatment ◽  
Dna Methylation Analysis ◽  
Conformation Polymorphism

scholarly journals Multiplexed DNA Methylation Analysis in Colorectal Cancer Using Liquid Biopsy and Its Diagnostic and Predictive Value

2021 ◽  
Vol 43 (3) ◽  
pp. 1419-1435
Author(s):  
Walter Pulverer ◽  
Kristi Kruusmaa ◽  
Silvia Schönthaler ◽  
Jasmin Huber ◽  
Marko Bitenc ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Therapy Monitoring ◽  
Methylation Status ◽  
Methylation Analysis ◽  
Microarray Experiments ◽  
Specific Pcr ◽  
Colonic Adenomas ◽  
Methylation Sensitive Restriction Enzyme ◽  
Dna Methylation Analysis

Early diagnosis of colorectal cancer (CRC) is of high importance as prognosis depends on tumour stage at the time of diagnosis. Detection of tumour-specific DNA methylation marks in cfDNA has several advantages over other approaches and has great potential for solving diagnostic needs. We report here the identification of DNA methylation biomarkers for CRC and give insights in our methylation-sensitive restriction enzyme coupled qPCR (MSRE-qPCR) system. Targeted microarrays were used to investigate the DNA methylation status of 360 cancer-associated genes. Validation was done by qPCR-based approaches. A focus was on investigating marker performance in cfDNA from 88 patients (44 CRC, 44 controls). Finally, the workflow was scaled-up to perform 180plex analysis on 110 cfDNA samples, to identify a DNA methylation signature for advanced colonic adenomas (AA). A DNA methylation signature (n = 44) was deduced from microarray experiments and confirmed by quantitative methylation-specific PCR (qMSP) and by MSRE-qPCR, providing for six genes’ single areas under the curve (AUC) values of >0.85 (WT1, PENK, SPARC, GDNF, TMEFF2, DCC). A subset of the signatures can be used for patient stratification and therapy monitoring for progressed CRC with liver metastasis using cfDNA. Furthermore, we identified a 35-plex classifier for the identification of AAs with an AUC of 0.80.

Identification of an episignature of human colorectal cancer associated with obesity by genome-wide DNA methylation analysis

2018 ◽  
Vol 43 (1) ◽  
pp. 176-188 ◽  
Author(s):  
Ana B. Crujeiras ◽  
Sonsoles Morcillo ◽  
Angel Diaz-Lagares ◽  
Juan Sandoval ◽  
Daniel Castellano-Castillo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Human Colorectal Cancer ◽  
Methylation Analysis ◽  
Genome Wide ◽  
Dna Methylation Analysis

scholarly journals DNA methylation analysis of SFRP2, GATA4/5, NDRG4 and VIM for the detection of colorectal cancer in fecal DNA

2014 ◽  
Vol 8 (4) ◽  
pp. 1751-1756 ◽  
Author(s):  
HONGNA LU ◽  
SHILIANG HUANG ◽  
XIE ZHANG ◽  
DANPING WANG ◽  
XUESONG ZHANG ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Fecal Dna ◽  
Methylation Analysis ◽  
Dna Methylation Analysis

scholarly journals Value of fecal DNA methylation analysis in early diagnosis of colorectal cancer

2014 ◽  
Vol 22 (22) ◽  
pp. 3201
Author(s):  
Hu Zhang ◽  
You-Qing Zhu ◽  
Ya-Qiong Wu ◽  
Ping Zhang ◽  
Jun Jiang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Early Diagnosis ◽  
Fecal Dna ◽  
Methylation Analysis ◽  
Dna Methylation Analysis

Genome-wide DNA methylation analysis of formalin-fixed paraffin embedded colorectal cancer tissue

2014 ◽  
Vol 53 (7) ◽  
pp. 537-548 ◽  
Author(s):  
Troy D. Dumenil ◽  
Leesa F. Wockner ◽  
Mark Bettington ◽  
Diane M. McKeone ◽  
Kerenaftali Klein ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Cancer Tissue ◽  
Methylation Analysis ◽  
Colorectal Cancer Tissue ◽  
Genome Wide ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Dna Methylation Analysis ◽  
Formalin Fixed
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