Alteration of HERG Current Profile during the Cardiac Ventricular Action Potential, Following a Pore Mutation

1998 ◽  
Vol 253 (3) ◽  
pp. 719-724 ◽  
Author(s):  
Jules C. Hancox ◽  
Harry J. Witchel ◽  
Anthony Varghese
Keyword(s):  
Action Potential ◽  
Current Profile ◽  
Herg Current ◽  
Ventricular Action Potential

scholarly journals Probucol-induced hERG Channel Reduction can be Rescued by Matrine and Oxymatrinein vitro

2020 ◽  
Vol 25 (43) ◽  
pp. 4606-4612 ◽  
Author(s):  
Yuan-Qi Shi ◽  
Pan Fan ◽  
Guo-Cui Zhang ◽  
Yu-Hao Zhang ◽  
Ming-Zhu Li ◽  
...  
Keyword(s):  
Action Potential ◽  
Action Potential Duration ◽  
Surface Expression ◽  
Herg Channel ◽  
Delayed Rectifier ◽  
Patch Clamp Recording ◽  
Herg Current ◽  
Factor Specificity ◽  
Lowering Drug

Background: The human ether-a-go-go-related gene (hERG) potassium channel is the rapidly activating component of cardiac delayed rectifier potassium current (IKr), which is a crucial determinant of cardiac repolarization. The reduction of hERG current is commonly believed to cause Long QT Syndrome (LQTs). Probucol, a cholesterol-lowering drug, induces LQTs by inhibiting the expression of the hERG channel. Unfortunately, there is currently no effective therapeutic method to rescue probucol-induced LQTs. Methods: Patch-clamp recording techniques were used to detect the action potential duration (APD) and current of hERG. Western blot was performed to measure the expression levels of proteins. Results: In this study, we demonstrated that 1 μM matrine and oxymatrine could rescue the hERG current and hERG surface expression inhibited by probucol. In addition, matrine and oxymatrine significantly shortened the prolonged action potential duration induced by probucol in neonatal cardiac myocytes. We proposed a novel mechanism underlying the probucol induced decrease in the expression of transcription factor Specificity protein 1 (Sp1), which is an established transactivator of the hERG gene. We also demonstrated that matrine and oxymatrine were able to upregulate Sp1 expression which may be one of the possible mechanisms by which matrine and oxymatrine rescued probucol-induced hERG channel deficiency. Conclusion: Our current results demonstrate that matrine and oxymatrine could rescue probucol-induced hERG deficiency in vitro, which may lead to potentially effective therapeutic drugs for treating acquired LQT2 by probucol in the future.

scholarly journals Inhibition of HERG K + Current and Prolongation of the Guinea‐Pig Ventricular Action Potential by 4‐Aminopyridine

2003 ◽  
Vol 549 (3) ◽  
pp. 667-672 ◽  
Author(s):  
J. M. Ridley ◽  
J. T. Milnes ◽  
Y. H. Zhang ◽  
H. J. Witchel ◽  
J. C. Hancox
Keyword(s):  
Guinea Pig ◽  
Action Potential ◽  
K Current ◽  
Ventricular Action Potential

Mathematical model of the ventricular action potential and effects of isoproterenol-induced cardiac hypertrophy in rats

2020 ◽  
Vol 49 (5) ◽  
pp. 323-342
Author(s):  
Sevgi Şengül Ayan ◽  
Ahmet K. Sırcan ◽  
Mohamedou Abewa ◽  
Ahmet Kurt ◽  
Uğur Dalaman ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Action Potential ◽  
Cardiac Hypertrophy ◽  
Ventricular Action Potential

scholarly journals Contributions of HERG K+ current to repolarization of the human ventricular action potential

2008 ◽  
Vol 96 (1-3) ◽  
pp. 357-376 ◽  
Author(s):  
Martin Fink ◽  
Denis Noble ◽  
Laszlo Virag ◽  
Andras Varro ◽  
Wayne R. Giles
Keyword(s):  
Action Potential ◽  
K Current ◽  
Ventricular Action Potential
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