Membrane Type 1-Matrix Metalloproteinase Is Involved in the Formation of Hepatocyte Growth Factor/Scatter Factor-Induced Branching Tubules in Madin-Darby Canine Kidney Epithelial Cells

1998 ◽  
Vol 251 (3) ◽  
pp. 681-687 ◽  
Author(s):  
Yoshifumi Kadono ◽  
Kazushige Shibahara ◽  
Mikio Namiki ◽  
Yoh Watanabe ◽  
Motoharu Seiki ◽  
...  
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Hepatocyte Growth Factor ◽  
Madin Darby Canine Kidney ◽  
Scatter Factor ◽  
Membrane Type ◽  
Hepatocyte Growth ◽  
Darby Canine Kidney ◽  
Canine Kidney

scholarly journals Sequential Activation of ERK and Repression of JNK by Scatter Factor/Hepatocyte Growth Factor in Madin-Darby Canine Kidney Epithelial Cells

2000 ◽  
Vol 11 (11) ◽  
pp. 3751-3763 ◽  
Author(s):  
Réjane Paumelle ◽  
David Tulasne ◽  
Catherine Leroy ◽  
Jean Coll ◽  
Bernard Vandenbunder ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Cell Density ◽  
Transcriptional Response ◽  
Madin Darby Canine Kidney ◽  
Scatter Factor ◽  
Hepatocyte Growth ◽  
Darby Canine Kidney ◽  
Canine Kidney

The scattering of Madin-Darby canine kidney (MDCK) epithelial cells by scatter factor/hepatocyte growth factor (SF/HGF) is associated with transcriptional induction of the urokinase gene, which occurs essentially through activation of an EBS/AP1 response element. We have investigated the signal transduction pathways leading to this transcriptional response. We found that SF/HGF induces rapid and sustained phosphorylation of the extracellular signal-regulated kinase (ERK) MAPK while stimulating weakly and then repressing phosphorylation of the JUN N-terminal kinase (JNK) MAPK for several hours. This delayed repression of JNK was preceded by phosphorylation of the MKP2 phosphatase, and both MKP2 induction and JNK dephosphorylation were under the control of MEK, the upstream kinase of ERK. ERK and MKP2 stimulate the EBS/AP1-dependent transcriptional response to SF/HGF, but not JNK, which inhibits this response. We further demonstrated that depending on cell density, the RAS-ERK-MKP2 pathway controls this transrepressing effect of JNK. Together, these data demonstrate that in a sequential manner SF/HGF activates ERK and MKP2, which in turn dephosphorylates JNK. This sequence of events provides a model for efficient cell scattering by SF/HGF at low cell density.

scholarly journals JAM4 enhances hepatocyte growth factor-mediated branching and scattering of Madin-Darby canine kidney cells

2004 ◽  
Vol 9 (9) ◽  
pp. 811-819 ◽  
Author(s):  
Hiroki Mori ◽  
Susumu Hirabayashi ◽  
Madoka Shirasawa ◽  
Haruhiko Sugimura ◽  
Yutaka Hata
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Kidney Cells ◽  
Madin Darby Canine Kidney ◽  
Hepatocyte Growth ◽  
Darby Canine Kidney ◽  
Canine Kidney
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