Identification of a New High-Affinity Binding Protein for Neurotoxic Phospholipases A2

1998 ◽  
Vol 251 (1) ◽  
pp. 209-212 ◽  
Author(s):  
Nataša Vučemilo ◽  
Alenka Čopič ◽  
Franc Gubenšek ◽  
Igor Križaj
Keyword(s):  
Binding Protein ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
Phospholipases A2 ◽  
High Affinity

scholarly journals Agrin Is a High-affinity Binding Protein of Dystroglycan in Non-muscle Tissue

1998 ◽  
Vol 273 (1) ◽  
pp. 600-605 ◽  
Author(s):  
Matthias Gesemann ◽  
Andrea Brancaccio ◽  
Beat Schumacher ◽  
Markus A. Ruegg
Keyword(s):  
Muscle Tissue ◽  
Binding Protein ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

scholarly journals N-Acylethanolamine Signaling in Tobacco Is Mediated by a Membrane-Associated, High-Affinity Binding Protein

2003 ◽  
Vol 131 (4) ◽  
pp. 1781-1791 ◽  
Author(s):  
Swati Tripathy ◽  
Kathryn Kleppinger-Sparace ◽  
Richard A. Dixon ◽  
Kent D. Chapman
Keyword(s):  
Binding Protein ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

RAT MYOMETRIAL ACTIVITY IN VIVO: EFFECTS OF OESTRADIOL-17β AND PROGESTERONE IN RELATION TO THE CONCENTRATIONS OF CYTOPLASMIC PROGESTERONE RECEPTORS

1978 ◽  
Vol 78 (1) ◽  
pp. 103-117 ◽  
Author(s):  
SANDRA J. DOWNING ◽  
S. J. LYE ◽  
JANE M. C. BRADSHAW ◽  
D. G. PORTER
Keyword(s):  
Binding Protein ◽  
Progesterone Receptors ◽  
Affinity Binding ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
High Affinity Binding ◽  
High Affinity ◽  
Repeated Doses ◽  
In Vivo Effects

The amplitude, frequency and rate of rise of intra-uterine pressure cycles in rats (postpartum, ovariectomized) were unaffected by treatment with progesterone. Amplitude was also unaffected by a combination of treatments with progesterone and oestradiol-17β, which was adequate to ensure the survival of 84% of foetuses in ovariectomized pregnant rats. The failure of progesterone to influence myometrial activity could not be attributed to a lack of 'true' progesterone receptors since these were present in the myometria of the test animals in concentrations exceeding those of oestrous animals. Evidence was obtained which suggested that a high-affinity binding protein, different from the 'true' receptor may predominate in the myometrium of the pregnant rat. Oestradiol-17β in single or repeated doses of from 0·25 to 5 μg, however, was found to reduce the frequency of pressure cycles but to increase significantly their rate of rise of pressure. There was a latency of 6–8 h in these effects of oestradiol. The possibility that inhibition of the myometrium by oestrogen may play a part in the preparation for parturition is discussed.

Characteristics of high-affinity folate binding in human leukocytes.

1983 ◽  
Vol 29 (5) ◽  
pp. 869-870 ◽  
Author(s):  
J Holm ◽  
S I Hansen ◽  
J Lyngbye
Keyword(s):  
Ligand Binding ◽  
Binding Affinity ◽  
Binding Protein ◽  
Equilibrium Dialysis ◽  
Normal Subjects ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
Human Leukocytes ◽  
High Affinity ◽  
Positive Cooperativity

Abstract High-affinity binding of [3H]folate in leukocytes from normal subjects was studied in equilibrium dialysis experiments (pH 7.4, 37 degrees C). Binding displayed positive cooperativity, and the binding affinity increased with decreasing concentration of the binding protein. Both phenomena could be interpreted in terms of ligand binding to a polymerizing system where the affinity of ligand for the oligomer is greater than its affinity for the polymer prevailing at higher concentrations of the binding protein.

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