Selection of DNA Binding Sites for Human Transcriptional Regulator GATA-6

1998 ◽  
Vol 250 (3) ◽  
pp. 682-688 ◽  
Author(s):  
Yuko Sakai ◽  
Reiko Nakagawa ◽  
Ryuichiro Sato ◽  
Masatomo Maeda
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
Transcriptional Regulator ◽  
Dna Binding Sites ◽  
Selection Of

Selection of DNA binding sites by regulatory proteins

1987 ◽  
Vol 193 (4) ◽  
pp. 723-743 ◽  
Author(s):  
Otto G. Berg ◽  
Peter H. von Hippel
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
Regulatory Proteins ◽  
Dna Binding Sites ◽  
Selection Of

Selection of DNA binding sites by regulatory proteins

1988 ◽  
Vol 13 (6) ◽  
pp. 207-211 ◽  
Author(s):  
Otto G. Berg ◽  
Peter H. von Hippel
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
Regulatory Proteins ◽  
Dna Binding Sites ◽  
Selection Of

In vitro selection of DNA binding sites for ABF1 protein fromSaccharomyces cerevisiae

FEBS Letters ◽  
2005 ◽  
Vol 579 (20) ◽  
pp. 4535-4540 ◽  
Author(s):  
Rasa Beinoravičiūtė-Kellner ◽  
Georg Lipps ◽  
Gerhard Krauss
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
In Vitro Selection ◽  
Dna Binding Sites ◽  
Selection Of

scholarly journals Functional divergence between the half-sites of the DNA-binding sequence for the yeast transcriptional regulator Rap1p

1999 ◽  
Vol 341 (3) ◽  
pp. 477-482 ◽  
Author(s):  
Fátima-Zahra IDRISSI ◽  
Benjamin PIÑA
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
Functional Divergence ◽  
Consensus Sequence ◽  
Transcriptional Regulator ◽  
Dna Complexes ◽  
Dna Binding Sites ◽  
Binding Sequence ◽  
Half Site

The yeast transcriptional regulator Rap1p binds to the DNA consensus sequence ACACCCAYACAYYY. We have previously shown that DNA-binding sites in which all four Y (Y = T or C) positions were Ts (UASrpg sequences) synergized more efficiently to activate transcription than sequences in which all Ys were Cs (telomere sequences) [F.-Z. Idrissi, J. Fernández-Larrea and B. Piña (1998) J. Mol. Biol. 284, 925-935]. Here we provide evidence that the DNA consensus sequence for Rap1p behaves as a combination of two ACAYYY half-sites with different functionality, the presence of Ts in the second half-site being the determinant for the transcriptional behaviour of the UASrpg sequences. DNA structure in the different complexes with Rap1p varied from being relatively uniform to appear rather distorted, this also being dependent on the presence of Ts in the second half-site. These distortions did not cause sharp bends or kinks in the DNA molecule. Computer analysis suggests that high-affinity binding of Rap1p to UASrpg sequences requires a rearrangement of the C-terminal Myb domain of the protein. We propose that the structural alterations in Rap1p-DNA complexes, both in the DNA and in the protein, affect the transcription potential of the complex in an allosteric manner. We also propose that the dimeric nature of the Rap1 DNA-binding domain is a key structural feature that explains the disparate functions of its DNA-binding sites in vivo.

scholarly journals Identification of Preferred DNA-Binding Sites for the Thermus thermophilus Transcriptional Regulator SbtR by the Combinatorial Approach REPSA

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0159408 ◽  
Author(s):  
Michael W. Van Dyke ◽  
Matthew D. Beyer ◽  
Emily Clay ◽  
Kamir J. Hiam ◽  
Jonathan L. McMurry ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
Thermus Thermophilus ◽  
Transcriptional Regulator ◽  
Combinatorial Approach ◽  
Dna Binding Sites

High-resolution detection of DNA binding sites of the global transcriptional regulator GlxR in Corynebacterium glutamicum

Microbiology ◽  
2013 ◽  
Vol 159 (Pt_1) ◽  
pp. 12-22 ◽  
Author(s):  
Britta Jungwirth ◽  
Claudia Sala ◽  
Thomas A. Kohl ◽  
Swapna Uplekar ◽  
Jan Baumbach ◽  
...  
Keyword(s):  
High Resolution ◽  
Dna Binding ◽  
Corynebacterium Glutamicum ◽  
Binding Sites ◽  
Transcriptional Regulator ◽  
Dna Binding Sites
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