Molecular cDNA Cloning and Tissue Distribution of mRNA Encoding a Novel ATP-Binding Cassette (ABC) Half-Transporter

1998 ◽  
Vol 249 (1) ◽  
pp. 151-155 ◽  
Author(s):  
K.I. Hirsch-Ernst ◽  
S. Gaini-Rahimi ◽  
B.-P. Ernst ◽  
C. Schmitz-Salue ◽  
S. Blume ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Cdna Cloning ◽  
Atp Binding ◽  
Atp Binding Cassette

Cloning and tissue distribution of the ATP-binding cassette subfamily G member 2 gene in the marine pufferfish Takifugu rubripes

2020 ◽  
Vol 86 (5) ◽  
pp. 873-887
Author(s):  
Takuya Matsumoto ◽  
Saemi Kitajima ◽  
Chisato Yamamoto ◽  
Mitsuru Aoyagi ◽  
Yoshiharu Mitoma ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Atp Binding ◽  
Takifugu Rubripes ◽  
Atp Binding Cassette

scholarly journals Cloning, characterization and tissue distribution of the rat ATP-binding cassette (ABC) transporter ABC2/ABCA2

2000 ◽  
Vol 350 (3) ◽  
pp. 865-872 ◽  
Author(s):  
Li-Xia ZHAO ◽  
Cheng-Ji ZHOU ◽  
Arowu TANAKA ◽  
Masanori NAKATA ◽  
Takahiro HIRABAYASHI ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Abc Transporter ◽  
Abc Transporters ◽  
Immunoblot Analysis ◽  
Atp Binding ◽  
Transporter Protein ◽  
The Brain ◽  
Brain Examination ◽  
Atp Binding Cassette

The ABC1 (ABCA) subfamily of the ATP-binding cassette (ABC) transporter superfamily has a structural feature that distinguishes it from other ABC transporters. Here we report the cloning, molecular characterization and tissue distribution of ABC2/ABCA2, which belongs to the ABC1 subfamily. Rat ABC2 is a protein of 2434 amino acids that has 44.5%, 40.0% and 40.8% identity with mouse ABC1/ABCA1, human ABC3/ABCA3 and human ABCR/ABCA4 respectively. Immunoblot analysis showed that proteins of 260 and 250kDa were detected in COS-1 cells transfected with ABC2 having a haemagglutinin tag, while no band was detected in mock-transfected cells. After incubation with N-glycosidase F, the mobilities of the two proteins increased and a single band was detected, suggesting that ABC2 is a glycoprotein. Photoaffinity labelling with 8-azido-[α-32P]ATP confirmed that ATP binds to the ABC2 protein in the presence of Mg2+. RNA blot analysis showed that ABC2 mRNA is most abundant in rat brain. Examination of brain by in situ hybridization determined that ABC2 is expressed at high levels in the white matter, indicating that it is expressed in the oligodendrocytes. ABC2, therefore, is a glycosylated ABC transporter protein, and may play an especially important role in the brain. In addition, the N-terminal 60-amino-acid sequence of the human ABC1, which was missing from previous reports, has been determined.

scholarly journals Cloning, characterization and tissue distribution of the rat ATP-binding cassette (ABC) transporter ABC2/ABCA2

2000 ◽  
Vol 350 (3) ◽  
pp. 865 ◽  
Author(s):  
Li-Xia ZHAO ◽  
Cheng-Ji ZHOU ◽  
Arowu TANAKA ◽  
Masanori NAKATA ◽  
Takahiro HIRABAYASHI ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Abc Transporter ◽  
Atp Binding ◽  
Atp Binding Cassette

Immunolocalization and funtional role of scavenger receptor BI (SR-BI) and ATP-binding cassette A1 (ABC1)

2001 ◽  
Vol 120 (5) ◽  
pp. A678-A678
Author(s):  
I SUC ◽  
M BENDAYAN ◽  
E DELVIN ◽  
L BRISSETTE ◽  
C GAROFALO ◽  
...  
Keyword(s):  
Scavenger Receptor ◽  
Atp Binding ◽  
Scavenger Receptor Bi ◽  
Atp Binding Cassette
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