Prohormone Convertase 1 (PC1) When Expressed with Pro Cholecystokinin (Pro CCK) in L Cells Performs Three Endoproteolytic Cleavages Which Are Observed in Rat Brain and in CCK-Expressing Endocrine Cells in Culture, Including the Production of Glycine and Arginine Extended CCK8

1998 ◽  
Vol 248 (3) ◽  
pp. 538-541 ◽  
Author(s):  
Wenge Wang ◽  
Nigel P. Birch ◽  
Margery C. Beinfeld
Keyword(s):  
Rat Brain ◽  
Endocrine Cells ◽  
Prohormone Convertase ◽  
Cells In Culture ◽  
L Cells ◽  
Prohormone Convertase 1

scholarly journals Aquaporin 1 Is Important for Maintaining Secretory Granule Biogenesis in Endocrine Cells

2008 ◽  
Vol 22 (8) ◽  
pp. 1924-1934 ◽  
Author(s):  
Irina Arnaoutova ◽  
Niamh X. Cawley ◽  
Nimesh Patel ◽  
Taeyoon Kim ◽  
Trushar Rathod ◽  
...  
Keyword(s):  
Secretory Granule ◽  
Endocrine Cells ◽  
Cell Function ◽  
Hormone Secretion ◽  
Prohormone Convertase ◽  
Protein Levels ◽  
Prohormone Convertase 1 ◽  
Aqp1 Expression ◽  
Low Levels ◽  
Made In

Abstract Aquaporins (AQPs), a family of water channels expressed in epithelial cells, function to transport water in a bidirectional manner to facilitate transepithelial fluid absorption and secretion. Additionally, AQP1 and AQP5 are found in pancreatic zymogen granules and synaptic vesicles and are involved in vesicle swelling and exocytosis in exocrine cells and neurons. Here, we show AQP1 is in dense-core secretory granule (DCSG) membranes of endocrine tissue: pituitary and adrenal medulla. The need for AQP1 in endocrine cell function was examined by stable transfection of AQP1 antisense RNA into AtT20 cells, a pituitary cell line, to down-regulate AQP1 expression. These AQP1-deficient cells showed more than 60% depletion of DCSGs and significantly decreased DCSG protein levels, including proopiomelanocotin/pro-ATCH and prohormone convertase 1/3, but not non-DCSG proteins. Pulse-chase studies revealed that whereas DCSG protein synthesis was unaffected, approximately 50% of the newly synthesized proopiomelanocortin was degraded within 1 h. Low levels of ACTH were released upon stimulation, indicating that the small number of DCSGs that were made in the presence of the residual AQP1 were functionally competent for exocytosis. Analysis of anterior pituitaries from AQP1 knockout mice showed reduced prohormone convertase 1/3, carboxypeptidase E, and ACTH levels compared to wild-type mice demonstrating that our results observed in AtT20 cells can be extended to the animal model. Thus, AQP1 is important for maintaining DCSG biogenesis and normal levels of hormone secretion in pituitary endocrine cells.

scholarly journals Proglucagon Processing Profile in Canine L Cells Expressing Endogenous Prohormone Convertase 1/3 and Prohormone Convertase 2

Endocrinology ◽  
1999 ◽  
Vol 140 (10) ◽  
pp. 4800-4808 ◽  
Author(s):  
Anders B. Damholt ◽  
Alison M. J. Buchan ◽  
Jens J. Holst ◽  
Hans Kofod
Keyword(s):  
Prohormone Convertase ◽  
L Cells ◽  
Prohormone Convertase 1 ◽  
Prohormone Convertase 2

Induction of ER stress by variants of the prohormone convertase 1 (PCSK1)-gene

2013 ◽  
Vol 8 (S 01) ◽  
Author(s):  
S Behrendt ◽  
D Löffler ◽  
R Tauscher ◽  
A Körner
Keyword(s):  
Er Stress ◽  
Prohormone Convertase ◽  
Prohormone Convertase 1 ◽  
Pcsk1 Gene

O8 Une déficience partielle en prohormone convertase 1 confère une augmentation du risque de l’obésité

2010 ◽  
Vol 36 ◽  
pp. A2-A3
Author(s):  
H. Choquet ◽  
J. Creemers ◽  
M. Pigeyre ◽  
V. Vatin ◽  
B. Balkau ◽  
...  
Keyword(s):  
Prohormone Convertase ◽  
Prohormone Convertase 1

Expression and localization of prohormone convertase 1/3 (SPC3) in porcine ovary

2000 ◽  
Vol 57 (4) ◽  
pp. 361-365 ◽  
Author(s):  
Randall H. Renegar ◽  
Charles R. Owens ◽  
Guoli Dai ◽  
John P. McMurtry ◽  
Simon C.M. Kwok
Keyword(s):  
Prohormone Convertase ◽  
Prohormone Convertase 1

scholarly journals Furin and Prohormone Convertase 1/3 Are Major Convertases in the Processing of Mouse Pro-Growth Hormone-Releasing Hormone

Endocrinology ◽  
2004 ◽  
Vol 145 (4) ◽  
pp. 1961-1971 ◽  
Author(s):  
Arunangsu Dey ◽  
Christina Norrbom ◽  
Xiaorong Zhu ◽  
Jeffrey Stein ◽  
Chunling Zhang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Prohormone Convertase ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Prohormone Convertase 1
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