Osteoclast Differentiation Factor Mediates an Essential Signal for Bone Resorption Induced by 1α,25-Dihydroxyvitamin D3, Prostaglandin E2, or Parathyroid Hormone in the Microenvironment of Bone

1998 ◽  
Vol 246 (2) ◽  
pp. 337-341 ◽  
Author(s):  
Katsuyoshi Tsukii ◽  
Nobuyuki Shima ◽  
Shin-ichi Mochizuki ◽  
Kyoji Yamaguchi ◽  
Masahiko Kinosaki ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Prostaglandin E2 ◽  
Osteoclast Differentiation ◽  
Dihydroxyvitamin D3 ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor

Interaction between amrinone and parathyroid hormone on bone in culture

1982 ◽  
Vol 243 (6) ◽  
pp. E499-E504
Author(s):  
N. S. Krieger ◽  
P. H. Stern
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Direct Interaction ◽  
Inhibitory Effects ◽  
Dihydroxyvitamin D3 ◽  
Basal Bone ◽  
Cardiotonic Agent ◽  
Neonatal Mouse Calvaria ◽  
Dose Dependent ◽  
Stimulation Of

The cardiotonic agent amrinone has been postulated to directly affect Na-Ca exchange. Because stimulated bone resorption has been proposed to require Na-Ca exchange, we examined the effects of amrinone on bone. Amrinone inhibited release of Ca from neonatal mouse calvaria in organ culture stimulated by parathyroid hormone (PTH), 1,25-dihydroxyvitamin d3, or prostaglandin E2. Inhibition was dose dependent and maximal at 2 X 10(-4) M. The effect of amrinone differed from the inhibitory effects of calcitonin, ouabain, or nigericin in that 1) 6-h exposure to amrinone alone prevented the effect of subsequently added PTH; 2) amrinone was only partially effective if added after resorption was initiated by 24-h treatment with PTH; 3) coincubation with amrinone and PTH during the first 48 h of culture allowed for a response to PTH after amrinone was removed; no such protection by a stimulator occurred with ouabain or nigericin. Also submaximal concentrations of amrinone plus calcitonin, ouabain, or nigericin gave greater than additive inhibition of Ca release. Amrinone had no effect on basal bone cAMP or on the acute stimulation of cAMP by PTH. The results suggest that amrinone could have a more direct interaction with the pathway involved in stimulated bone resorption than the other inhibitors.

scholarly journals Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Ae Ri Kim ◽  
Yun Kyong Lim ◽  
Joong-Ki Kook ◽  
Eun-Jung Bak ◽  
Yun-Jung Yoo
Keyword(s):  
Flow Cytometry ◽  
Porphyromonas Gingivalis ◽  
Osteoclast Differentiation ◽  
Fusobacterium Nucleatum ◽  
Pathological Changes ◽  
Air Pouch ◽  
E Coli ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor

AbstractIncreases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not been clarified yet. We evaluated numerical changes of RANKL-expressing neutrophils in air pouches of mice injected with LPSs of Fusobacterium nucleatum and Porphyromonas gingivalis. Mice with air pouches were assigned into saline (C)-, E. coli LPS- (Ec LPS)-, F. nucleatum LPS (Fn LPS)-, P. gingivalis LPS (Pg LPS)-, and Fn LPS and Pg LPS (Fn + Pg LPS)-injected groups. CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils in blood and air pouch exudates were determined by flow cytometry. In blood, compared to the C group, the Fn LPS group showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils whereas the Pg LPS group showed no significant differences. These increases in the Fn LPS group were not different to those in the Ec LPS group. In exudates, Fn LPS and Pg LPS groups showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils compared to the C group. Increased levels in the Fn LPS group were not different to those in the Ec LPS group, but Pg LPS group was lower than those in the Ec LPS group. In blood and exudates, the Fn + Pg LPS group showed no difference in levels of these neutrophils compared to the Ec LPS group. LPSs of F. nucleatum and P. gingivalis increased RANKL-expressing neutrophils although the degrees of increases were different. These suggest that periodontopathogen LPS can act as a stimulant to increase RANKL-expressing neutrophils.

scholarly journals Gene Expression of Osteoclast Differentiation Factor Is Induced by Lipopolysaccharide in Mouse Osteoblasts Via Toll-Like Receptors

2001 ◽  
Vol 166 (5) ◽  
pp. 3574-3579 ◽  
Author(s):  
Takeshi Kikuchi ◽  
Tetsuya Matsuguchi ◽  
Naotake Tsuboi ◽  
Akio Mitani ◽  
Shigehisa Tanaka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Osteoclast Differentiation ◽  
Toll Like Receptors ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor

Low Calcium Environment Effects Osteoprotegerin Ligand/Osteoclast Differentiation Factor

2000 ◽  
Vol 276 (2) ◽  
pp. 524-529 ◽  
Author(s):  
Sadaaki Takeyama ◽  
Yoshitaka Yoshimura ◽  
Yoichi Shirai ◽  
Yoshiaki Deyama ◽  
Tomokazu Hasegawa ◽  
...  
Keyword(s):  
Osteoclast Differentiation ◽  
Environment Effects ◽  
Low Calcium ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor ◽  
Osteoprotegerin Ligand
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