Down-Regulation of Human Sialyltransferase Gene Expression during in Vitro Human Keratinocyte Cell Line Differentiation

1998 ◽  
Vol 243 (1) ◽  
pp. 177-183 ◽  
Author(s):  
Akiyoshi Taniguchi ◽  
Kojiro Matsumoto
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Down Regulation ◽  
Human Keratinocyte ◽  
Human Keratinocyte Cell Line ◽  
Keratinocyte Cell

Malignant progression of an HPV16-immortalized human keratinocyte cell line (HPKIA) in vitro

1995 ◽  
Vol 85 (2) ◽  
pp. 105-112 ◽  
Author(s):  
Matthias Dürst ◽  
Sibylle Seagon ◽  
Sylke Wanschura ◽  
Harald zur Hausen ◽  
Jörn Bullerdiek
Keyword(s):  
Cell Line ◽  
Malignant Progression ◽  
Human Keratinocyte ◽  
Human Keratinocyte Cell Line ◽  
Keratinocyte Cell

scholarly journals Local Treatment of Hand-Foot Syndrome with Uridine/Thymidine:In VitroAppraisal on a Human Keratinocyte Cell Line HaCaT

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
J. Hartinger ◽  
P. Veselý ◽  
E. Matoušková ◽  
S. Argalacsová ◽  
L. Petruželka ◽  
...  
Keyword(s):  
Cell Line ◽  
Local Treatment ◽  
Local Therapy ◽  
Anticancer Therapy ◽  
Protective Activity ◽  
Human Keratinocyte ◽  
Human Keratinocyte Cell Line ◽  
Keratinocyte Cell Line Hacat ◽  
Keratinocyte Cell

5-fluorouracil (5-FU) is one of the most commonly used antineoplastic drugs in the anticancer therapy. The hand-foot (HF) syndrome (palmar-plantar erythrodysesthesia) is an adverse effect frequently related to long-term i.v. administration of 5-FU or its orally applicable prodrug capecitabine. Its severity can even lead to interruption of the otherwise effective anticancer therapy. Tentative practice in some clinics has shown that topical application of 10% uridine ointment is beneficial for calming down the HF syndrome. This study is focused on verifying the alleged protective activity of uridine in thein vitromodel of cultured human keratinocyte cell line HaCaT. We also tested the protective effects of thymidine alone or uridine-thymidine combination. The cellular viability time progression was measured in order to evaluate the effect of protective agents by three different types of cytopathogenicity tests—NTCA test (non-destructive test of cellular activity), modified MTT test and RTCA (real-time cell analyser, Roche). All three methods proved the ability of uridine and uridine-thymidine combination to protect keratinocytes against 5-FU damagein vitro. While thymidine alone did not show any remarkable effect, the thymidine-uridine combination demonstrated enhanced protective activity compared to uridine alone. Our findings provided the supporting rationale for using uridine or uridine-thymidine ointments in the HF syndrome local therapy.

Benzoyl peroxide cytotoxicity evaluated in vitro with the human keratinocyte cell line, RHEK-1

Toxicology ◽  
1996 ◽  
Vol 106 (1-3) ◽  
pp. 187-196 ◽  
Author(s):  
H. Babich ◽  
H.L. Zuckerbraun ◽  
B.J. Wurzburger ◽  
Y.L. Rubin ◽  
E. Borenfreund ◽  
...  
Keyword(s):  
Cell Line ◽  
Benzoyl Peroxide ◽  
Human Keratinocyte ◽  
Human Keratinocyte Cell Line ◽  
Keratinocyte Cell

Implementation of an in vitro methodology for phototoxicity evaluation in a human keratinocyte cell line

2019 ◽  
Vol 61 ◽  
pp. 104618 ◽  
Author(s):  
B. Maciel ◽  
P. Moreira ◽  
H. Carmo ◽  
M. Gonçalo ◽  
J.M. Sousa Lobo ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Keratinocyte ◽  
Human Keratinocyte Cell Line ◽  
Keratinocyte Cell
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