Molecular Characterization of the Glucose-Sensing Mechanism in the Clonal Insulin-Secreting BRIN-BD11 Cell Line

1998 ◽  
Vol 242 (2) ◽  
pp. 262-266 ◽  
Author(s):  
Neville H. McClenaghan ◽  
Matthias Elsner ◽  
Markus Tiedge ◽  
Sigurd Lenzen
Keyword(s):  
Cell Line ◽  
Molecular Characterization ◽  
Glucose Sensing ◽  
Sensing Mechanism

scholarly journals Molecular characterization of a defensin in the IZD-MB-0503 cell line derived from immunocytes of the insect Mamestra brassicae (Lepidoptera)

2003 ◽  
Vol 95 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Mauro Mandrioli ◽  
Silvia Bugli ◽  
Sabrina Saltini ◽  
Susanna Genedani ◽  
Enzo Ottaviani
Keyword(s):  
Cell Line ◽  
Molecular Characterization ◽  
Mamestra Brassicae

Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS

1991 ◽  
Vol 86 (6) ◽  
Author(s):  
G�nter Rudolph ◽  
Karin Schilbach-St�ckle ◽  
Rupert Handgretinger ◽  
Peter Kaiser ◽  
Horst Hameister
Keyword(s):  
Cell Line ◽  
Molecular Characterization ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line

Biological and Molecular Characterization by Integrative Genomics Approach of CMA-03/06, a Newly Established Interleukin-6 Independent Variant of the CMA-03 Human Myeloma Cell Line.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1814-1814
Author(s):  
Donata Verdelli ◽  
Lucia Nobili ◽  
Katia Todoerti ◽  
Laura Mosca ◽  
Sonia Fabris ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Molecular Characterization ◽  
Cell Lines ◽  
Stromal Cells ◽  
Interleukin 6 ◽  
Myeloma Cell ◽  
Growth And Survival ◽  
Genome Wide

Abstract Abstract 1814 Poster Board I-840 Background The growth and survival of multiple myeloma (MM) cells in the bone marrow microenvironment is regulated by functional complex interactions between the tumor cells and the surrounding bone marrow stromal cells mediated by adhesion molecules and the production of several cytokines of which interleukin-6 (IL-6) has been identified as the most important. Major advances in the investigation of MM biology were made possible by the availability of human myeloma cell lines (HMCLs). The IL-6-dependent CMA-03 cell line was established in our laboratory from a peritoneal effusion of a refractory relapsed MM patient. By gradually decreasing the IL-6 added to the culture, an IL-6-independent variant, CMA-03/06, could be obtained. Aims. To perform a biological and molecular characterization of this novel cell line, and to provide insights into the signaling pathways and target genes involved in the growth and survival of CMA-03/06. Methods. The growth, immunophenotypic, cytogenetic and fluorescence in situ hybridization (FISH) characterization of CMA-03/06 cell line was performed by means of standard procedures. IL-6 production into the culture media was determined using a high sensitivity IL-6 specific ELISA. Genome-wide profiling data were generated by means of Affymetrix GeneChip® Human Mapping 250K Nsp arrays; copy number (CN) alterations were calculated using the DNAcopy Bioconductor package, based on circular binary segmentation method. Global gene expression profiling (GEP) was performed by means of the GeneChip® Human Gene 1.0 ST Arrays (Affymetrix); the supervised analyses were done using the SAM software version 3.0. Results Unlike CMA-03, the addition of IL-6 to the culture medium of CMA-03/06 cells or co-culture with multipotent mesenchymal stromal cells did not induce an increase in CMA-03/06 proliferation. IL-6 was not detected in the supernatants from either CMA-03 or CMA-03/06 cell lines within 48 h, suggesting that the IL-6 independence of CMA03/06 cells is not a result of the development of an autocrine IL-6 loop. Nevertheless, IL-6 induced the activation of STAT3 and STAT1 in both cell lines, even if a slight constitutive STAT3 phosphorylation was found in CMA-03/06. The immunophenotypic analysis showed a significant difference in the expression of three antigens in the 2 cell lines: CD45 was considerably reduced in CMA-03/06 cells, whereas they were found positive for both chains of IL-6 receptor, CD126 and CD130, almost undetectable in CMA-03. Conventional cytogenetic and FISH analyses did not reveal differences between the 2 HMCLs. The genome-wide analysis allowed the identification of about 100 altered chromosomal regions common to both HMCLs, mostly DNA gains. Comparison of CMA-03/06 and CMA-03 cells evidenced a different CN in only 15 small chromosomal regions, 8 of which did not contain any transcript, whereas few genes were located on the other ones. GEP analysis of CMA-03/06 compared with CMA-03 identified 21 upregulated and 47 downregulated genes, many of which particularly relevant for MM biology, mainly involved in cellular signaling, cell cycle, cell adhesion, cell development, regulation of transcription, immunologic, inflammatory or defense activity, apoptosis. None of the genes differentially expressed in CMA-03/06 compared with CMA-03 except 1 were positioned on the chromosomal regions showing a different CN. Finally, CMA-03/06 cell line showed a lower susceptibility to camptothecin-induced apoptosis compared to CMA-03 cells. Conclusions Our data show the IL-6 independence of CMA-03/06 cell line in the absence of an autocrine IL-6 loop; the cells, however, maintain the IL-6 signaling pathway responsiveness. A consistent number of genes particularly relevant for MM biology were found deregulated in CMA-03/06 cell line compared with CMA-03. Furthermore, CMA-03/06 cell line shows an increased resistance to apoptosis. The novel CMA03/06 cell line may thus represent a suitable model for studies investigating molecular mechanisms involved in clonal evolution towards IL-6 and/or stroma-independent growth and survival of myeloma cells. Disclosures No relevant conflicts of interest to declare.

Phenotypic, cytogenetic and molecular characterization of a new B-chronic lymphocytic leukaemia (B-CLL) cell line

1987 ◽  
Vol 11 (7) ◽  
pp. 579-588 ◽  
Author(s):  
Federico Caligaris-Cappio ◽  
Luciana Bergui ◽  
Giovanna Rege-Cambrin ◽  
Luisa Tesio ◽  
Nicola Migone ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Cell Line ◽  
Molecular Characterization ◽  
Lymphocytic Leukaemia

scholarly journals A Novel Glucose-Sensing Mechanism Contributing to Glucagon-Like Peptide-1 Secretion From the GLUTag Cell Line

Diabetes ◽  
2003 ◽  
Vol 52 (5) ◽  
pp. 1147-1154 ◽  
Author(s):  
F. M. Gribble ◽  
L. Williams ◽  
A. K. Simpson ◽  
F. Reimann
Keyword(s):  
Cell Line ◽  
Glucose Sensing ◽  
Sensing Mechanism ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

scholarly journals Cellular and molecular characterization of an embryonic cell line (BME26) from the tick Rhipicephalus (Boophilus) microplus

2008 ◽  
Vol 38 (5) ◽  
pp. 568-580 ◽  
Author(s):  
Eliane Esteves ◽  
Flavio A. Lara ◽  
Daniel M. Lorenzini ◽  
Gustavo H.N. Costa ◽  
Aline H. Fukuzawa ◽  
...  
Keyword(s):  
Cell Line ◽  
Molecular Characterization ◽  
Embryonic Cell ◽  
Boophilus Microplus ◽  
Embryonic Cell Line
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