The Conserved Serine-Threonine-Serine Motif of the Carnitine Acyltransferases Is Involved in Carnitine Binding and Transition-State Stabilization: A Site-Directed Mutagenesis Study

1997 ◽  
Vol 241 (3) ◽  
pp. 803-804 ◽  
Author(s):  
Ciarán N. Cronin
Keyword(s):  
Transition State ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Transition State Stabilization ◽  
Carnitine Acyltransferases ◽  
A Site ◽  
Mutagenesis Study

scholarly journals A site-directed mutagenesis study of human placental aromatase.

1992 ◽  
Vol 267 (2) ◽  
pp. 762-768
Author(s):  
D J Zhou ◽  
K R Korzekwa ◽  
T Poulos ◽  
S A Chen
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
A Site ◽  
Mutagenesis Study

Functional tyrosine residue in the active center of human dipeptidyl peptidase III

2008 ◽  
Vol 389 (2) ◽  
pp. 163-167 ◽  
Author(s):  
Branka Salopek-Sondi ◽  
Bojana Vukelić ◽  
Jasminka Špoljarić ◽  
Šumski Šimaga ◽  
Dušica Vujaklija ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transition State ◽  
Active Site ◽  
Functional Role ◽  
Dipeptidyl Peptidase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Tyrosine Residue ◽  
Transition State Stabilization ◽  
Endogenous Defense

Abstract Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49 with an implied role in the pain-modulatory system and endogenous defense against oxidative stress. Here, we report the heterologous expression of human DPP III and the site-directed mutagenesis results which demonstrate a functional role for Tyr318 at the active site of this enzyme. The substitution of Tyr318 to Phe decreased k cat by two orders of magnitude without altering the binding affinity of substrate, or of a competitive hydroxamate inhibitor designed to interact with S1 and S2 subsites. The results indicate that the conserved tyrosine could be involved in transition state stabilization during the catalytic action of M49 peptidases.

Role of Acidic and Aromatic Amino Acids inRhodobacter CapsulatusCytochromec1. A Site-Directed Mutagenesis Study†

Biochemistry ◽  
2003 ◽  
Vol 42 (29) ◽  
pp. 8818-8830 ◽  
Author(s):  
Jun Li ◽  
Artur Osyczka ◽  
Richard C. Conover ◽  
Michael K. Johnson ◽  
Hong Qin ◽  
...  
Keyword(s):  
Amino Acids ◽  
Aromatic Amino Acids ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
A Site ◽  
Mutagenesis Study

A Site-Directed Mutagenesis Study of Yeast Calmodulin1

1991 ◽  
Vol 109 (1) ◽  
pp. 190-197 ◽  
Author(s):  
Isao Matsuura ◽  
Kazushi Ishihara ◽  
Yoriko Nakai ◽  
Michio Yazawa ◽  
Hiroko Toda ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
A Site ◽  
Mutagenesis Study
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