Expression of Wnt5a Is Downregulated by Extracellular Matrix and Mutated c-Ha-ras in the Human Mammary Epithelial Cell Line MCF-10A

1997 ◽  
Vol 239 (3) ◽  
pp. 911-917 ◽  
Author(s):  
Thuan D. Bui ◽  
Giampaolo Tortora ◽  
Fortunato Ciardiello ◽  
Adrian L. Harris
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Human Mammary Epithelial

scholarly journals Characterization of immortalized human mammary epithelial cell line HMEC 2.6

Tumor Biology ◽  
2017 ◽  
Vol 39 (10) ◽  
pp. 101042831772428 ◽  
Author(s):  
Pooja S Joshi ◽  
Vishnu Modur ◽  
JiMing Cheng ◽  
Kathy Robinson ◽  
Krishna Rao
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Human Mammary Epithelial

282. Anti-apoptotic effects of glucocorticoids and progesterone in a human mammary epithelial cell line

2005 ◽  
Vol 17 (9) ◽  
pp. 118
Author(s):  
M. N. Berg ◽  
B. J. Waddell ◽  
A. M. Dharmarajan
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Human Mammary Epithelial ◽  
Apoptotic Effects

Previous studies in the lactating rat mammary gland have shown that removal of both progesterone and glucocorticoids can induce apoptosis, and that replacement with either steroid alone can prevent this effect.1 These results raised the possibility that progesterone may exert its anti-apoptotic effects in the mammary gland via the glucocorticoid receptor (GR). A similar mechanism has previously been proposed to account for the action of progesterone in the corpus luteum.2 To assess this possibility we examined the effects of glucocorticoids and progesterone on apoptosis in the non-tumorigenic human mammary epithelial cell line, MCF-10A. Initial PCR analysis demonstrated that MCF-10A cells do not express progesterone receptor mRNA (PR-A or PR-B). MCF-10A cells were incubated in serum-free DMEM:F12 medium with additives in the presence or absence of cortisol (10–6M) or progesterone (10–5M to 10–8M). DNA fragmentation (an index of apoptosis) was quantitated by either 3′-end labelling or by a novel method of image analysis of SYBR® gold-stained gels. DNA fragmentation was clearly increased after 24 h in the absence of cortisol, consistent with previous reports for this cell line.3 In a separate experiment, replacement of cortisol with progesterone also reduced apoptosis by 48 h, although this protection was evident only at the two highest progesterone doses (10–5M and 10–6M). These results suggest that despite the absence of a progesterone receptor, progesterone is able to prevent apoptosis in MCF-10A cells, albeit at a physiologically high doses. This supports the hypothesis that some actions of progesterone may be mediated via the GR. Further studies are required to determine the precise molecular pathways by which cortisol and progesterone prevent apoptosis. (1)Berg MN et al. (2002) Endocrinology 143, 222–227.(2)Sugino N et al. (1997) Endocrinology 138, 4497–4500.(3)Moran TJ et al. (2000) Cancer Res 60, 867–872.

Sign in / Sign up

Export Citation Format

Share Document