Isolation and Characterization of Mitochondrial DNA-less Lines from Various Mammalian Cell Lines by Application of an Anticancer Drug, Ditercalinium

1997 ◽  
Vol 239 (1) ◽  
pp. 257-260 ◽  
Author(s):  
Kimiko Inoue ◽  
Daisaku Takai ◽  
Hideka Hosaka ◽  
Sayaka Ito ◽  
Hiroshi Shitara ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Anticancer Drug ◽  
Mammalian Cell Lines ◽  
Isolation And Characterization

Isolation and characterization of mammalian cell lines carrying suppressible mutations

1988 ◽  
Vol 213 (1) ◽  
pp. 105-111
Author(s):  
Rubén López-Revilla ◽  
Herminia G. Martínez ◽  
María del Socorro Santos-Díaz
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Mammalian Cell Lines ◽  
Isolation And Characterization

Characterization of cell-banking parameters for the cryopreservation of mammalian cell lines in 100-mL cryobags

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Rüdiger Heidemann ◽  
Svenja Lünse ◽  
Doan Tran ◽  
Chun Zhang
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Mammalian Cell Lines ◽  
Cell Banking

scholarly journals Establishment and Characterization of Mammalian Cell Lines Stably Expressing Human L-Type Amino Acid Transporters

2008 ◽  
Vol 108 (4) ◽  
pp. 505-516 ◽  
Author(s):  
Emiko Morimoto ◽  
Yoshikatsu Kanai ◽  
Do Kyung Kim ◽  
Arthit Chairoungdua ◽  
Hye Won Choi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Amino Acid Transporters ◽  
Mammalian Cell Lines

Expression and characterization of equine herpesvirus 1 glycoprotein D in mammalian cell lines

1996 ◽  
Vol 141 (9) ◽  
pp. 1785-1793 ◽  
Author(s):  
J. E. Wellington ◽  
G. L. Lawrence ◽  
D. N. Love ◽  
J. M. Whalley
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Equine Herpesvirus ◽  
Glycoprotein D ◽  
Equine Herpesvirus 1 ◽  
Mammalian Cell Lines ◽  
Herpesvirus 1

scholarly journals ESTABLISHMENT AND CHARACTERIZATION OF ETHIDIUM BROMIDE RESISTANCE IN SIMIAN VIRUS 40-TRANSFORMED HAMSTER CELLS

1973 ◽  
Vol 58 (1) ◽  
pp. 11-26 ◽  
Author(s):  
Wolfgang Klietmann ◽  
Nobuhiro Sato ◽  
Margit M. K. Nass
Keyword(s):  
Mitochondrial Dna ◽  
Cell Line ◽  
Cell Lines ◽  
Ethidium Bromide ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Ultrastructural Changes ◽  
Parental Cell Line ◽  
Mammalian Cell Lines

This study describes the isolation and subsequent characterization of four mammalian cell lines resistant to ethidium bromide (EB). Treatment of the simian virus 40- (SV40) transformed hamster cell line F5-1 first led to the establishment of the F2 cell line, which is resistant to 2 µg EB/ml. At this concentration cytochromes c and b are present in almost normal or only slightly diminished amounts, whereas cytochromes a + a3 show an obvious decrease. The mitochondria of the F2 cell show a normal ultrastructure, not distinct from the parental cell line F5-1, and contain closed circular DNA. The sensitive parental F5-1 cells, however, when exposed to the same dye concentration exhibit the typical EB-induced ultrastructural changes in the mitochondria, and no more component I mitochondrial DNA can be demonstrated. 1 yr after establishment we derived from the F2 cell three more cell lines, resistant against 4, 8, and 16 µg of EB/ml. These cell lines, termed F4, F8, and F16, respectively, also revealed relatively intact-appearing mitochondria, although distinguishable from F5-1 and F2 mitochondria by a more condensed or unorthodox cristae conformation. F4, F8, and F16 cell lines contained closed circular mitochondrial DNA in the same position as that of the parental F5-1 cells, when analyzed in an isopycnic CsCl-EB gradient. A small shoulder at the lower density side of the DNA I peaks was observed. The newly acquired drug resistance of the F cells is hereditarily transmitted to the progeny cells and retained even after a period of growth in EB-free medium.

Expression and characterization of Cys374 mutated human β-actin in two different mammalian cell lines: impaired microfilament organization and stability

FEBS Letters ◽  
1999 ◽  
Vol 455 (1-2) ◽  
pp. 117-122 ◽  
Author(s):  
A Tsapara ◽  
D Kardassis ◽  
A Moustakas ◽  
A Gravanis ◽  
C Stournaras
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Mammalian Cell Lines
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