Identification of Macrophage Migration Inhibitory Factor in Adipose Tissue and Its Induction by Tumor Necrosis Factor-α

1997 ◽  
Vol 235 (1) ◽  
pp. 94-98 ◽  
Author(s):  
Junichi Hirokawa ◽  
Shinji Sakaue ◽  
Seiichi Tagami ◽  
Yoshikazu Kawakami ◽  
Masaharu Sakai ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Tumor Necrosis Factor ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Factor Α ◽  
Necrosis Factor

Role of Macrophage Migration Inhibitory Factor in Paranasal Sinus Mucocele

2005 ◽  
Vol 19 (6) ◽  
pp. 554-559 ◽  
Author(s):  
Shin Kariya ◽  
Mitsuhiro Okano ◽  
Katsuya Aoji ◽  
Tomoko Nakashima ◽  
Norio Kasai ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Paranasal Sinus ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Tumor Necrosis ◽  
Inhibitory Factor ◽  
Interleukin 1Β ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Necrosis Factor

Background Little is known about the immunologic aspects and the pathogenesis of the paranasal sinus mucocele. Methods The fluids of paranasal sinus mucoceles were obtained from 12 subjects. The concentration of macrophage migration inhibitory factor (MIF), interleukin 1β, tumor necrosis factor a, and regulated on activation normal T cell expressed and secreted (RANTES) were determined by enzyme-linked immunosorbent assay, and the levels of endotoxin were detected with kinetic Turbidimetric Assay. Results MIF and endotoxin were detected in the fluid of all samples, whereas interleukin-1β and RANTES were detected in 1 and 3 subjects out of 12 samples. Tumor necrosis factor a was not detected in any of the samples. A significant positive correlation between the levels of MIF and the period with symptoms such as pain, swelling of face, and visual disturbance was observed. Conclusion These findings suggest that MIF and endotoxin may play an important role in the pathogenesis of paranasal sinus mucocele. MIF may be an important factor causing the development and exacerbation of the disease.

scholarly journals Regulation of Interleukin 8 Production and Gene Expression in Human Adipose Tissue in Vitro1

2001 ◽  
Vol 86 (3) ◽  
pp. 1267-1273 ◽  
Author(s):  
Jens M. Bruun ◽  
Steen B. Pedersen ◽  
Bjørn Richelsen
Keyword(s):  
Adipose Tissue ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Human Adipose Tissue ◽  
Interleukin 8 ◽  
Isolated Adipocytes ◽  
Factor Α ◽  
Necrosis Factor

A variety of cytokines and other compounds are produced in the human adipose tissue and may have autocrine functions in the adipose tissue as well as be involved in the complications seen in association with obesity. Because it recently has been reported that interleukin 8 (IL-8), through its effects on the macrophage and endothelial cell, may be involved in the pathogenesis of atherosclerosis, we found it of interest to investigate whether IL-8 is produced in human adipose tissue in vitro. Human sc adipose tissue was investigated both in incubations with whole adipose tissue fragments as well as with isolated mature adipocytes. In adipose tissue fragments, IL-1β (3 nm) and tumor necrosis factor α (0.6 nm) were able to stimulate IL-8 production by 12-fold and 5-fold, respectively (P < 0.001), when incubated for 48 h. Incubations with isolated adipocytes were performed up to 6 h, and IL-1β and tumor necrosis factor α significantly increased IL-8 production by 50–60% (P < 0.05). Dexamethasone (50 nm) decreased IL-8 production from adipose tissue fragments by 57% (P < 0.01) and from adipocytes by 37% (P < 0.05). IL-8 messenger RNA expression in adipocytes incubated with IL-1β was increased already after 2 h (P < 0.05). Thus, the effect of proinflammatory cytokines and dexamethasone on IL-8 production in adipose tissue seems to be mediated at the transcriptional level. In conclusion, it is demonstrated for the first time that IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.

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