High-Affinity Peptide Ligands to Prostate-Specific Antigen Identified by Polysome Selection

1997 ◽  
Vol 232 (2) ◽  
pp. 578-582 ◽  
Author(s):  
Geoffrey M. Gersuk ◽  
Michael J. Corey ◽  
Eva Corey ◽  
James E. Stray ◽  
Glenn H. Kawasaki ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Peptide Ligands ◽  
High Affinity ◽  
Affinity Peptide

scholarly journals Semenogelins I and II bind zinc and regulate the activity of prostate-specific antigen

2005 ◽  
Vol 387 (2) ◽  
pp. 447-453 ◽  
Author(s):  
Magnus JONSSON ◽  
Sara LINSE ◽  
Birgitta FROHM ◽  
Åke LUNDWALL ◽  
Johan MALM
Keyword(s):  
Enzymatic Activity ◽  
Dissociation Constant ◽  
Prostate Specific Antigen ◽  
Protease Activity ◽  
Specific Antigen ◽  
Active State ◽  
Chromogenic Substrate ◽  
Nmr Analysis ◽  
High Concentration ◽  
High Affinity

In semen, the gel proteins SgI and SgII (semenogelins I and II) are digested by PSA (prostate-specific antigen), resulting in liquefaction and release of motile spermatozoa. Semen contains a high concentration of Zn2+, which is known to inhibit the protease activity of PSA. We characterized the binding of Zn2+ to SgI and SgII and found evidence that these proteins are involved in regulating the activity of PSA. Intact SgI and SgII and synthetic semenogelin peptides were used in the experiments. Binding of Zn2+ was studied by radioligand blotting, titration with a zinc (II) fluorophore chelator and NMR analysis. A chromogenic substrate was used to measure the enzymatic activity of PSA. SgI and SgII bound Zn2+ with a stoichiometry of at least 10 mol (mol of protein)−1 and with an average dissociation constant of approx. 5 μM per site. Moreover, Zn2+-inhibited PSA was activated by exposure to SgI or SgII. Since both proteins have high affinity for Zn2+ and are the dominating proteins in semen, they probably represent the major Zn2+ binders in semen, one function of which may be to regulate the activity of PSA. The system is self-regulating, and PSA is maintained in an active state by its substrate.

Rational Design of Specific High-Affinity Peptide Ligands for the Abl-SH3 Domain†

Biochemistry ◽  
1996 ◽  
Vol 35 (33) ◽  
pp. 10634-10640 ◽  
Author(s):  
M. T. Pisabarro ◽  
L. Serrano
Keyword(s):  
Rational Design ◽  
Sh3 Domain ◽  
Peptide Ligands ◽  
High Affinity ◽  
Affinity Peptide

Selection of high affinity peptide ligands for detection of circulating antibodies in neurocysticercosis

2010 ◽  
Vol 129 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Vanessa da Silva Ribeiro ◽  
Marianna Nascimento Manhani ◽  
Rone Cardoso ◽  
Carlos Ueira Vieira ◽  
Luiz Ricardo Goulart ◽  
...  
Keyword(s):  
Peptide Ligands ◽  
High Affinity ◽  
Circulating Antibodies ◽  
Affinity Peptide ◽  
Selection Of

Discovery of high-affinity peptide ligands for vancomycin

Biopolymers ◽  
2008 ◽  
Vol 90 (3) ◽  
pp. 421-432 ◽  
Author(s):  
Nianhuan Yao ◽  
Chun-Yi Wu ◽  
Wenwu Xiao ◽  
Kit S. Lam
Keyword(s):  
Peptide Ligands ◽  
High Affinity ◽  
Affinity Peptide

Using Molecular Repertoires to Identify High-Affinity Peptide Ligands of the WW Domain of Human and Mouse YAP

1997 ◽  
Vol 378 (6) ◽  
Author(s):  
Hillary Linn ◽  
Kira S. Ermekova ◽  
Stacey Rentschier ◽  
Andrew B. Sparks ◽  
Brian K. Kay ◽  
...  
Keyword(s):  
Peptide Ligands ◽  
Ww Domain ◽  
High Affinity ◽  
Affinity Peptide ◽  
Human And Mouse

scholarly journals Pancreatic β-Cell Imaging with High Affinity Peptide Ligands to the GLP-1 Receptor

2015 ◽  
Author(s):  
Bikash Manandhar ◽  
William Silvers ◽  
Amit Kumar ◽  
Su-Tang Lo ◽  
Xiankai Sun ◽  
...  
Keyword(s):  
Cell Imaging ◽  
Peptide Ligands ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
High Affinity ◽  
Affinity Peptide

Design of new high-affinity peptide ligands for human leukocyte antigen-DQ2 using a positional scanning peptide library

2010 ◽  
Vol 71 (5) ◽  
pp. 475-481 ◽  
Author(s):  
Ulrike Jüse ◽  
Yvonne van de Wal ◽  
Frits Koning ◽  
Ludvig M. Sollid ◽  
Burkhard Fleckenstein
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Peptide Library ◽  
Peptide Ligands ◽  
Leukocyte Antigen ◽  
High Affinity ◽  
Positional Scanning ◽  
Affinity Peptide
Sign in / Sign up

Export Citation Format

Share Document