Upregulation of Actin Gene Expression in Cells Expressing Exogenous β-Amyloid Precursor Protein

1997 ◽  
Vol 231 (3) ◽  
pp. 615-618 ◽  
Author(s):  
Narayan Ramakrishna ◽  
Marilyn Smedman ◽  
Vasundhara Ramakrishna ◽  
Bruce Gillam
Keyword(s):  
Gene Expression ◽  
Amyloid Precursor Protein ◽  
Precursor Protein ◽  
Actin Gene ◽  
Β Amyloid ◽  
In Cells

scholarly journals Transcriptional Regulation of BACE1, the β-Amyloid Precursor Protein β-Secretase, by Sp1

2004 ◽  
Vol 24 (2) ◽  
pp. 865-874 ◽  
Author(s):  
Michelle A. Christensen ◽  
Weihui Zhou ◽  
Hong Qing ◽  
Anna Lehman ◽  
Sjaak Philipsen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Amyloid Precursor Protein ◽  
Proteolytic Processing ◽  
Precursor Protein ◽  
Transcriptional Level ◽  
Response Element ◽  
App Processing ◽  
Transcription Factor Sp1 ◽  
Β Amyloid

ABSTRACT Proteolytic processing of the β-amyloid precursor protein (APP) at the β site is essential to generate Aβ. BACE1, the major β-secretase involved in cleaving APP, has been identified as a type 1 membrane-associated aspartyl protease. We have cloned a 2.1-kb fragment upstream of the human BACE1 gene and identified key regions necessary for promoter activity. BACE1 gene expression is controlled by a TATA-less promoter. The region of bp −619 to +46 is the minimal promoter to control the transcription of the BACE1 gene. Several putative cis-acting elements, such as a GC box, HSF-1, a PU box, AP1, AP2, and lymphokine response element, are found in the 5′ flanking region of the BACE1 gene. Transcriptional activation and gel shift assays demonstrated that the BACE1 promoter contains a functional Sp1 response element, and overexpression of the transcription factor Sp1 potentiates BACE gene expression and APP processing to generate Aβ. Furthermore, Sp1 knockout reduced BACE1 expression. These results suggest that BACE1 gene expression is tightly regulated at the transcriptional level and that the transcription factor Sp1 plays an important role in regulation of BACE1 to process APP generating Aβ in Alzheimer's disease.

scholarly journals Exchange of N-CoR Corepressor and Tip60 Coactivator Complexes Links Gene Expression by NF-κB and β-Amyloid Precursor Protein

Cell ◽  
2002 ◽  
Vol 110 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Sung Hee Baek ◽  
Kenneth A. Ohgi ◽  
David W. Rose ◽  
Edward H. Koo ◽  
Christopher K. Glass ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amyloid Precursor Protein ◽  
Precursor Protein ◽  
Β Amyloid
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