Myosin Binding Subunit of Smooth Muscle Myosin Phosphatase at the Cell-Cell Adhesion Sites in MDCK Cells

1997 ◽  
Vol 230 (3) ◽  
pp. 552-556 ◽  
Author(s):  
Naoyuki Inagaki ◽  
Miwako Nishizawa ◽  
Masaaki Ito ◽  
Masaki Fujioka ◽  
Takeshi Nakano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Adhesion ◽  
Mdck Cells ◽  
Smooth Muscle Myosin ◽  
Myosin Phosphatase ◽  
Adhesion Sites ◽  
Myosin Binding ◽  
Muscle Myosin ◽  
Binding Subunit ◽  
Cell Cell

scholarly journals Characterization of the myosin-binding subunit of smooth muscle myosin phosphatase.

1994 ◽  
Vol 269 (48) ◽  
pp. 30407-30411
Author(s):  
H Shimizu ◽  
M Ito ◽  
M Miyahara ◽  
K Ichikawa ◽  
S Okubo ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Myosin Phosphatase ◽  
Myosin Binding ◽  
Muscle Myosin ◽  
Binding Subunit

scholarly journals Altered Expression of Human Smooth Muscle Myosin Phosphatase Targeting (MYPT) Isovariants with Pregnancy and Labor

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164352 ◽  
Author(s):  
Jon Lartey ◽  
Julie Taggart ◽  
Stephen Robson ◽  
Michael Taggart
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Myosin Phosphatase ◽  
Altered Expression ◽  
Muscle Myosin

scholarly journals Interactions of the Subunits of Smooth Muscle Myosin Phosphatase

1997 ◽  
Vol 272 (6) ◽  
pp. 3683-3688 ◽  
Author(s):  
Katsuya Hirano ◽  
Brigitte C. Phan ◽  
David J. Hartshorne
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Myosin Phosphatase ◽  
Muscle Myosin

scholarly journals Recruitment of E-cadherin associated with α- and β-catenins and p120ctn to the nectin-based cell-cell adhesion sites by the action of 12-O-tetradecanoylphorbol-13-acetate in MDCK cells

2005 ◽  
Vol 10 (5) ◽  
pp. 435-445 ◽  
Author(s):  
Ryoko Okamoto ◽  
Kenji Irie ◽  
Akio Yamada ◽  
Tatsuo Katata ◽  
Atsunori Fukuhara ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Mdck Cells ◽  
Adhesion Sites ◽  
E Cadherin ◽  
Cell Cell

scholarly journals Smooth muscle myosin phosphatase inhibition and force enhancement by black sponge toxin

FEBS Letters ◽  
1987 ◽  
Vol 217 (1) ◽  
pp. 81-84 ◽  
Author(s):  
Akira Takai ◽  
Corinna Bialojan ◽  
Monika Troschka ◽  
J. Caspar Rüegg
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Force Enhancement ◽  
Myosin Phosphatase ◽  
Phosphatase Inhibition ◽  
Muscle Myosin

scholarly journals Assembly of Smooth Muscle Myosin by the 38k Protein, a Homologue of a Subunit of Pre-mRNA Splicing Factor-2

2000 ◽  
Vol 148 (4) ◽  
pp. 653-664 ◽  
Author(s):  
Tsuyoshi Okagaki ◽  
Akio Nakamura ◽  
Tomohiko Suzuki ◽  
Kazuhiro Ohmi ◽  
Kazuhiro Kohama
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Smooth Muscles ◽  
Muscle Cells ◽  
Smooth Muscle Myosin ◽  
Mrna Splicing ◽  
Splicing Factor ◽  
A Subunit ◽  
Myosin Binding ◽  
Muscle Myosin

Smooth muscle myosin in the dephosphorylated state does not form filaments in vitro. However, thick filaments, which are composed of myosin and myosin-binding protein(s), persist in smooth muscle cells, even if myosin is subjected to the phosphorylation– dephosphorylation cycle. The characterization of telokin as a myosin-assembling protein successfully explained the discrepancy. However, smooth muscle cells that are devoid of telokin have been observed. We expected to find another ubiquitous protein with a similar role, and attempted to purify it from chicken gizzard. The 38k protein bound to both phosphorylated and dephosphorylated myosin to a similar extent. The effect of the myosin-binding activity was to assemble dephosphorylated myosin into filaments, although it had no effect on the phosphorylated myosin. The 38k protein bound to myosin with both COOH-terminal 20 and NH2-terminal 28 residues of the 38k protein being essential for myosin binding. The amino acid sequence of the 38k protein was not homologous to telokin, but to human p32, which was originally found in nuclei as a subunit of pre-mRNA splicing factor-2. Western blotting showed that the protein was expressed in various smooth muscles. Immunofluorescence microscopy with cultured smooth muscle cells revealed colocalization of the 38k protein with myosin and with other cytoskeletal elements. The absence of nuclear immunostaining was discussed in relation to smooth muscle differentiation.

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