Long-Term Human T-Cell Culture System Supporting Hepatitis C Virus Replication

1996 ◽  
Vol 227 (3) ◽  
pp. 822-826 ◽  
Author(s):  
Tetsuya Mizutani ◽  
Nobuyuki Kato ◽  
Masanori Ikeda ◽  
Kazuo Sugiyama ◽  
Kunitada Shimotohno
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
T Cell ◽  
Virus Replication ◽  
Culture System ◽  
Cell Culture System ◽  
Human T Cell

Serum-free cell culture system supplemented with lipid-rich albumin for hepatitis C virus (strain O of genotype 1b) replication

2007 ◽  
Vol 125 (2) ◽  
pp. 162-168 ◽  
Author(s):  
Ken-ichi Abe ◽  
Masanori Ikeda ◽  
Yasuo Ariumi ◽  
Hiromichi Dansako ◽  
Nobuyuki Kato
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Strain ◽  
Culture System ◽  
Free Cell ◽  
Cell Culture System ◽  
Serum Free ◽  
Genotype 1B

Study of multiple genetic variations caused by persistent hepatitis C virus replication in long-term cell culture

2019 ◽  
Vol 165 (2) ◽  
pp. 331-343 ◽  
Author(s):  
Nobuyuki Kato ◽  
Youki Ueda ◽  
Hiroe Sejima ◽  
Weilin Gu ◽  
Shinya Satoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Genetic Variations

scholarly journals Serum amyloid A has antiviral activity against hepatitis C virus by inhibiting virus entry in a cell culture system

Hepatology ◽  
2006 ◽  
Vol 44 (6) ◽  
pp. 1626-1634 ◽  
Author(s):  
Muriel Lavie ◽  
Cécile Voisset ◽  
Ngoc Vu-Dac ◽  
Virginie Zurawski ◽  
Gilles Duverlie ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Antiviral Activity ◽  
Culture System ◽  
Serum Amyloid A ◽  
Virus Entry ◽  
Cell Culture System ◽  
Amyloid A ◽  
A Cell

scholarly journals Fungus-Derived Neoechinulin B as a Novel Antagonist of Liver X Receptor, Identified by Chemical Genetics Using a Hepatitis C Virus Cell Culture System

2016 ◽  
Vol 90 (20) ◽  
pp. 9058-9074 ◽  
Author(s):  
Syo Nakajima ◽  
Koichi Watashi ◽  
Hirofumi Ohashi ◽  
Shinji Kamisuki ◽  
Jesus Izaguirre-Carbonell ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Culture System ◽  
Membrane Vesicles ◽  
Chemical Genetics ◽  
Liver X Receptor ◽  
Bioactive Molecules ◽  
Cell Culture System ◽  
Double Membrane

ABSTRACTCell culture systems reproducing virus replication can serve as unique models for the discovery of novel bioactive molecules. Here, using a hepatitis C virus (HCV) cell culture system, we identified neoechinulin B (NeoB), a fungus-derived compound, as an inhibitor of the liver X receptor (LXR). NeoB was initially identified by chemical screening as a compound that impeded the production of infectious HCV. Genome-wide transcriptome analysis and reporter assays revealed that NeoB specifically inhibits LXR-mediated transcription. NeoB was also shown to interact directly with LXRs. Analysis of structural analogs suggested that the molecular interaction of NeoB with LXR correlated with the capacity to inactivate LXR-mediated transcription and to modulate lipid metabolism in hepatocytes. Our data strongly suggested that NeoB is a novel LXR antagonist. Analysis using NeoB as a bioprobe revealed that LXRs support HCV replication: LXR inactivation resulted in dispersion of double-membrane vesicles, putative viral replication sites. Indeed, cells treated with NeoB showed decreased replicative permissiveness for poliovirus, which also replicates in double-membrane vesicles, but not for dengue virus, which replicates via a distinct membrane compartment. Together, our data suggest that LXR-mediated transcription regulates the formation of virus-associated membrane compartments. Significantly, inhibition of LXRs by NeoB enhanced the activity of all known classes of anti-HCV agents, and NeoB showed especially strong synergy when combined with interferon or an HCV NS5A inhibitor. Thus, our chemical genetics analysis demonstrates the utility of the HCV cell culture system for identifying novel bioactive molecules and characterizing the virus-host interaction machinery.IMPORTANCEHepatitis C virus (HCV) is highly dependent on host factors for efficient replication. In the present study, we used an HCV cell culture system to screen an uncharacterized chemical library. Our results identified neoechinulin B (NeoB) as a novel inhibitor of the liver X receptor (LXR). NeoB inhibited the induction of LXR-regulated genes and altered lipid metabolism. Intriguingly, our results indicated that LXRs are critical to the process of HCV replication: LXR inactivation by NeoB disrupted double-membrane vesicles, putative sites of viral replication. Moreover, NeoB augmented the antiviral activity of all known classes of currently approved anti-HCV agents without increasing cytotoxicity. Thus, our strategy directly links the identification of novel bioactive compounds to basic virology and the development of new antiviral agents.

scholarly journals Establishment of interferon alpha-resistant hepatitis C virus using cell culture system

FEBS Letters ◽  
2010 ◽  
Vol 585 (2) ◽  
pp. 409-413 ◽  
Author(s):  
Huong T.L. Tran ◽  
Yun-Sook Lim ◽  
Soon B. Hwang
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Interferon Alpha ◽  
Culture System ◽  
Cell Culture System

scholarly journals Viral Load Analysis of Hepatitis C Virus in Huh7.5 Cell Culture System

2015 ◽  
Vol 8 (5) ◽  
Author(s):  
Roghayeh Teimourpour ◽  
Zahra Meshkat ◽  
Aida Gholoubi ◽  
Hosein Nomani ◽  
Sina Rostami
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Viral Load ◽  
Hepatitis C ◽  
Culture System ◽  
Cell Culture System ◽  
Load Analysis

Evaluation of antiviral effects of novel NS5A inhibitors in hepatitis C virus cell culture system with full-genome infectious clones

2018 ◽  
Vol 158 ◽  
pp. 161-170
Author(s):  
Asako Murayama ◽  
Kei Fujiwara ◽  
Norie Yamada ◽  
Masaaki Shiina ◽  
Hussein Hassan Aly ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Culture System ◽  
Full Genome ◽  
Cell Culture System ◽  
Antiviral Effects ◽  
Infectious Clones
Sign in / Sign up

Export Citation Format

Share Document