Oxidative Stress Increases Gene Expression of Heparin-Binding EGF-like Growth Factor and Amphiregulin in Cultured Rat Gastric Epithelial Cells

1996 ◽  
Vol 226 (2) ◽  
pp. 542-546 ◽  
Author(s):  
Yoshiji Miyazaki ◽  
Yasuhisa Shinomura ◽  
Shusaku Tsutsui ◽  
Yuichi Yasunaga ◽  
Shinichiro Zushi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Heparin Binding ◽  
Gastric Epithelial Cells

Bronchial epithelial compression regulates MAP kinase signaling and HB-EGF-like growth factor expression

2002 ◽  
Vol 282 (5) ◽  
pp. L904-L911 ◽  
Author(s):  
Daniel J. Tschumperlin ◽  
Jonathan D. Shively ◽  
Melody A. Swartz ◽  
Eric S. Silverman ◽  
Kathleen J. Haley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Compressive Stress ◽  
Bronchial Epithelial Cells ◽  
Heparin Binding ◽  
Bronchial Epithelial ◽  
Extracellular Signal ◽  
Normal Human ◽  
Kinase Phosphorylation

Airway smooth muscle constriction leads to the development of compressive stress on bronchial epithelial cells. Normal human bronchial epithelial cells exposed to an apical-to-basal transcellular pressure difference equivalent to the computed stress in the airway during bronchoconstriction demonstrate enhanced phosphorylation of extracellular signal-regulated kinase (ERK). The response is pressure dependent and rapid, with phosphorylation increasing 14-fold in 30 min, and selective, since p38 and c-Jun NH2-terminal kinase phosphorylation remains unchanged after pressure application. Transcellular pressure also elicits a ninefold increase in expression of mRNA encoding heparin-binding epidermal growth factor-like growth factor (HB-EGF) after 1 h, followed by prominent immunostaining for pro-HB-EGF after 6 h. Inhibition of the ERK pathway with PD-98059 results in a dose-dependent reduction in pressure-induced HB-EGF gene expression. The magnitude of the HB-EGF response to transcellular pressure and tumor necrosis factor (TNF)-α (1 ng/ml) is similar, and the combined mechanical and inflammatory stimulus is more effective than either stimulus alone. These results demonstrate that compressive stress is a selective and potent activator of signal transduction and gene expression in bronchial epithelial cells.

Heparin-Binding EGF-like Growth Factor Is an Autocrine Growth Factor for Rat Gastric Epithelial Cells

1996 ◽  
Vol 223 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Yoshiji Miyazaki ◽  
Yasuhisa Shinomura ◽  
Shigeki Higashiyama ◽  
Shuji Kanayama ◽  
Yoshifumi Higashimoto ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Heparin Binding ◽  
Gastric Epithelial Cells ◽  
Autocrine Growth ◽  
Autocrine Growth Factor

Gastrin induces heparin-binding epidermal growth factor–like growth factor in rat gastric epithelial cells transfected with gastrin receptor

1999 ◽  
Vol 116 (1) ◽  
pp. 78-89 ◽  
Author(s):  
Yoshiji Miyazaki ◽  
Yasuhisa Shinomura ◽  
Shusaku Tsutsui ◽  
Shinichiro Zushi ◽  
Yoshifumi Higashimoto ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Heparin Binding ◽  
Gastric Epithelial Cells ◽  
Gastrin Receptor ◽  
Epidermal Growth

Gastrin induces heparin-binding EGF-like growth factor in rat gastric epithelial cells transfected with gastrin receptor

1998 ◽  
Vol 114 ◽  
pp. A896
Author(s):  
Yoshiji Miyazaki ◽  
Yasuhisa Shinomura ◽  
Shusaku TSutsui ◽  
Shinichiro Zushi ◽  
Yoshifumi Higashimoto ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Heparin Binding ◽  
Gastric Epithelial Cells ◽  
Gastrin Receptor

Eupatilin Protects Gastric Epithelial Cells from Oxidative Damage and Down-Regulates Genes Responsible for the Cellular Oxidative Stress

2008 ◽  
Vol 25 (6) ◽  
pp. 1355-1364 ◽  
Author(s):  
Eun-Ju Choi ◽  
Hyun-Mee Oh ◽  
Bo-Ra Na ◽  
T. P. Ramesh ◽  
Hyun-Ju Lee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Oxidative Damage ◽  
Gastric Epithelial Cells
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