Differential Expression of Protein Tyrosine Kinases in the Cortical and Medullary Thymic Epithelial Cell Lines

1995 ◽  
Vol 213 (3) ◽  
pp. 768-773 ◽  
Author(s):  
T. Kuwata ◽  
H. Takahashi ◽  
M. Kasai ◽  
C. Kurashima ◽  
M. Utsuyama ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Differential Expression ◽  
Cell Lines ◽  
Tyrosine Kinases ◽  
Protein Tyrosine Kinases ◽  
Thymic Epithelial Cell ◽  
Protein Tyrosine ◽  
Epithelial Cell Lines ◽  
Medullary Thymic Epithelial Cell

CD10 is expressed on human thymic epithelial cell lines and modulates thymopentin‐induced cell proliferation

1997 ◽  
Vol 11 (12) ◽  
pp. 1003-1011 ◽  
Author(s):  
Sandrine Guérin ◽  
Bernard Mari ◽  
Edgar Fernandez ◽  
Nathalie Belhacene ◽  
Maria Luisa Toribio ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Thymic Epithelial Cell ◽  
Epithelial Cell Lines

Mouse thymic epithelial cell lines expressing “Aire” and peripheral tissue-specific antigens reproduce in vitro negative selection of T cells

2011 ◽  
Vol 317 (14) ◽  
pp. 2019-2030 ◽  
Author(s):  
Yoshitaka Yamaguchi ◽  
Atsushi Takayanagi ◽  
Jiabing Chen ◽  
Kosuke Sakai ◽  
Jun Kudoh ◽  
...  
Keyword(s):  
T Cells ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Negative Selection ◽  
Thymic Epithelial Cell ◽  
Tissue Specific ◽  
Epithelial Cell Lines ◽  
Specific Antigens ◽  
Selection Of

Peptide-amidating Enzymes Are Expressed in the Stellate Epithelial Cells of the Thymic Medulla

1998 ◽  
Vol 46 (5) ◽  
pp. 661-668 ◽  
Author(s):  
Alfredo Martínez ◽  
Andrew Farr ◽  
Michele D. Vos ◽  
Frank Cuttitta ◽  
Anthony M. Treston
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Lines ◽  
B Lymphocyte ◽  
Regulatory Peptides ◽  
Convincing Evidence ◽  
Thymic Epithelial Cells ◽  
Thymic Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Amidating Enzymes

C-terminal amidation is a post-translational processing step necessary to convey biological activity to a large number of regulatory peptides. In this study we have demonstrated that the peptidyl-glycine α-amidating monooxygenase enzyme complex (PAM) responsible for this activity is located in the medullary stellate epithelial cells of the thymus and in cultured epithelial cells bearing a medullary phenotype, using Northern blot, immunocytochemistry, in situ hybridization, and enzyme assays. Immunocytochemical localization revealed a granular pattern in the cytoplasm of the stellate cells, which were also positive for cytokeratins and a B-lymphocyte-associated antigen. The presence of PAM activity in medium conditioned by thymic epithelial cell lines suggests that PAM is a secreted product of these cells. Among the four epithelial cell lines examined, there was a direct correlation between PAM activity and content of oxytocin, an amidated peptide. Taken together, these data provide convincing evidence that thymic epithelial cells have the capacity to generate amidated peptides that may influence T-cell differentiation and suggest that the amidating enzymes could play an important role in the regulation of thymic physiology.

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