The 5′-Untranslated Region of Human Transferrin mRNA, Which Contains a Putative Iron-Regulatory Element, Is Bound by Purified Iron-Regulatory Protein in a Sequence-Specific Manner

1995 ◽  
Vol 212 (3) ◽  
pp. 925-932 ◽  
Author(s):  
L.A. Cox ◽  
M.C. Kennedy ◽  
G.S. Adrian
Keyword(s):  
Untranslated Region ◽  
Regulatory Element ◽  
Regulatory Protein ◽  
Iron Regulatory Protein ◽  
Human Transferrin ◽  
Specific Manner ◽  
Iron Regulatory Element

Cell-autonomous and systemic context-dependent functions of iron regulatory protein 2 in mammalian iron metabolism

Blood ◽  
2009 ◽  
Vol 113 (3) ◽  
pp. 679-687 ◽  
Author(s):  
Dunja Ferring-Appel ◽  
Matthias W. Hentze ◽  
Bruno Galy
Keyword(s):  
Regulatory Protein ◽  
Intrinsic Defect ◽  
Iron Regulatory Protein ◽  
Iron Loading ◽  
Body Iron ◽  
Splenic Macrophages ◽  
Iron Parameters ◽  
Context Dependent ◽  
Blood Cell And Plasma

Abstract Mice with total and constitutive iron regulatory protein 2 (IRP2) deficiency exhibit microcytosis and altered body iron distribution with duodenal and hepatic iron loading and decreased iron levels in splenic macrophages. To explore cell-autonomous and systemic context-dependent functions of IRP2 and to assess the systemic consequences of local IRP2 deficiency, we applied Cre/Lox technology to specifically ablate IRP2 in enterocytes, hepatocytes, or macrophages, respectively. This study reveals that the hepatic and duodenal manifestations of systemic IRP2 deficiency are largely explained by cell-autonomous functions of IRP2. By contrast, IRP2-deficient macrophages from otherwise IRP2-sufficient mice do not display the abnormalities of macrophages from systemically IRP2-deficient animals, suggesting that these result from IRP2 disruption in other cell type(s). Mice with enterocyte-, hepatocyte-, or macrophage-specific IRP2 deficiency display normal red blood cell and plasma iron parameters, supporting the notion that the microcytosis in IRP2-deficient mice likely reflects an intrinsic defect in hematopoiesis. This work defines the respective roles of IRP2 in the determination of critical body iron parameters such as organ iron loading and erythropoiesis.

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