Differential Expression of Wild-Type and Variant ER mRNAs by Stocks of MCF-7 Breast Cancer Cells May Account for Differences in Estrogen Responsiveness

1995 ◽  
Vol 210 (2) ◽  
pp. 609-615 ◽  
Author(s):  
D.M. Klotz ◽  
C.G. Castles ◽  
S.A.W. Fuqua ◽  
L.L. Spriggs ◽  
S.M. Hill
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Differential Expression ◽  
Breast Cancer Cells ◽  
Wild Type ◽  
Mcf 7

scholarly journals Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells

2021 ◽  
Vol 22 (15) ◽  
pp. 8165
Author(s):  
Amanda Chantziou ◽  
Kostas Theodorakis ◽  
Hara Polioudaki ◽  
Eelco de Bree ◽  
Marilena Kampa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Plasma Membrane ◽  
Subcellular Localization ◽  
Cancer Cells ◽  
Membrane Trafficking ◽  
Breast Cancer Cells ◽  
Endocytic Pathway ◽  
Cell Periphery ◽  
Wild Type ◽  
Mcf 7

In breast cancer, expression of Cluster of Differentiation 24 (CD24), a small GPI-anchored glycoprotein at the cell periphery, is associated with metastasis and immune escape, while its absence is associated with tumor-initiating capacity. Since the mechanism of CD24 sorting is unknown, we investigated the role of glycosylation in the subcellular localization of CD24. Expression and localization of wild type N36- and/or N52-mutated CD24 were analyzed using immunofluorescence in luminal (MCF-7) and basal B (MDA-MB-231 and Hs578T) breast cancer cells lines, as well as HEK293T cells. Endogenous and exogenously expressed wild type and mutated CD24 were found localized at the plasma membrane and the cytoplasm, but not the nucleoplasm. The cell lines showed different kinetics for the sorting of CD24 through the secretory/endocytic pathway. N-glycosylation, especially at N52, and its processing in the Golgi were critical for the sorting and expression of CD24 at the plasma membrane of HEK293T and basal B type cells, but not of MCF-7 cells. In conclusion, our study highlights the contribution of N-glycosylation for the subcellular localization of CD24. Aberrant N-glycosylation at N52 of CD24 could account for the lack of CD24 expression at the cell surface of basal B breast cancer cells.

scholarly journals Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

2011 ◽  
Vol 313 (1) ◽  
pp. 26-43 ◽  
Author(s):  
Tissa T. Manavalan ◽  
Yun Teng ◽  
Savitri N. Appana ◽  
Susmita Datta ◽  
Theodore S. Kalbfleisch ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Differential Expression ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Microrna Expression ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Tamoxifen Resistant ◽  
Mcf 7

Alteration of caspase-3 (CPP32/Yama/apopain) in wild-type MCF-7, breast cancer cells.

1999 ◽  
Author(s):  
H Kurokawa ◽  
K Nishio ◽  
H Fukumoto ◽  
A Tomonari ◽  
T Suzuki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Caspase 3 ◽  
Wild Type ◽  
Mcf 7

Differential expression and androgen regulation of microRNA molecules in breast cancer cells

2016 ◽  
Author(s):  
Mamoun Ahram ◽  
Rand Zaza ◽  
Ebtihal Mustafa ◽  
Heba Jarrar ◽  
Razan Al-Saber ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Differential Expression ◽  
Breast Cancer Cells ◽  
Androgen Regulation
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