Cationic Multilamellar Liposome-Mediated Gene Transfer into Primary Myoblasts

1995 ◽  
Vol 207 (1) ◽  
pp. 8-12 ◽  
Author(s):  
H. Kojima ◽  
N. Ohishi ◽  
M. Takamori ◽  
K. Yagi
Keyword(s):  
Gene Transfer ◽  
Multilamellar Liposome ◽  
Primary Myoblasts

scholarly journals Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer

2000 ◽  
Vol 74 (8) ◽  
pp. 3555-3565 ◽  
Author(s):  
Ajay K. Malik ◽  
Paul E. Monahan ◽  
David L. Allen ◽  
Bing-Guan Chen ◽  
R. Jude Samulski ◽  
...  
Keyword(s):  
Gene Transfer ◽  
High Efficiency ◽  
Factor Ix ◽  
Lag Phase ◽  
Cmv Promoter ◽  
Adeno Associated Virus ◽  
Primary Myoblasts ◽  
Human Factor Ix ◽  
Hmw Dna ◽  
Kinetics Of

ABSTRACT Recombinant adeno-associated virus (rAAV) vectors have been shown to be useful for efficient gene delivery to a variety of dividing and nondividing cells. Mechanisms responsible for the long-term, persistent expression of the rAAV transgene are not well understood. In this study we investigated the kinetics of rAAV-mediated human factor IX (hFIX) gene transfer into human primary myoblasts and myotubes. Transduction of both myoblasts and myotubes occured with a similar and high efficiency. After 3 to 4 weeks of transduction, rAAV with a cytomegalovirus (CMV) promoter showed 10- to 15-fold higher expression than that with a muscle-specific creatine kinase enhancer linked to β-actin promoter. Factor IX expression from transduced myoblasts as well as myotubes reached levels as high as approximately 2 μg of hFIX/106 cells/day. Southern blot analyses of high-molecular-weight (HMW) cellular genomic and Hirt DNAs isolated from rAAV/CMVhFIXm1-transduced cells showed that the conversion of single-stranded vector genomes to double-stranded DNA forms, but not the level of the integrated forms in HMW DNA, correlated with increasing expression of the transgene. Together, these results indicate that rAAV can transduce both proliferating and terminally differentiated muscle cells at about the same efficiency, that expression of transgenes increases linearly over their lifetime with no initial lag phase, and that increasing expression correlates with the appearance of double-stranded episomal rAAV genomes. Evidence showing that the rAAV virions can copackage hFIX, presumably nonspecifically, was also obtained.

Repeated cationic multilamellar liposome-mediated gene transfer enhanced transduction efficiency against murine melanoma cell lines

2002 ◽  
Vol 29 (3) ◽  
pp. 206-213 ◽  
Author(s):  
Misato Nobayashi ◽  
Masaaki Mizuno ◽  
Toshiro Kageshita ◽  
Kazuhiko Matsumoto ◽  
Toshiaki Saida ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Transduction Efficiency ◽  
Multilamellar Liposome ◽  
Murine Melanoma ◽  
Murine Melanoma Cell ◽  
Melanoma Cell Lines

Efficient non-viral DNA-mediated gene transfer to human primary myoblasts using electroporation

2001 ◽  
Vol 11 (4) ◽  
pp. 341-349 ◽  
Author(s):  
E Espinos ◽  
J.H Liu ◽  
C.R Bader ◽  
L Bernheim
Keyword(s):  
Gene Transfer ◽  
Viral Dna ◽  
Primary Myoblasts

Implanted myoblasts not only fuse with myofibers but also survive as muscle precursor cells

1993 ◽  
Vol 105 (4) ◽  
pp. 957-963 ◽  
Author(s):  
S.N. Yao ◽  
K. Kurachi
Keyword(s):  
Gene Transfer ◽  
Muscle Regeneration ◽  
Precursor Cells ◽  
Additional Mechanism ◽  
Myogenic Cells ◽  
Muscle Precursor ◽  
Primary Myoblasts ◽  
Muscle Precursor Cells

Intramuscular implanted myoblasts can fuse with existing myofibers. Here we report that implanted primary myoblasts marked with retroviral transgenes can also persist as muscle precursor cells. These cells can be recovered as viable myoblasts from muscles of recipient mice even months after myoblast implantation, and they can fully resume expression of the transgenes in culture. Upon re-implantation into muscles, they again not only fuse with existing myofibers, but also survive as muscle precursor cells in the tissue. These reserve myogenic cells should be able to contribute to host myofibers in muscle regeneration when the recombinant myofibers are damaged, providing an additional mechanism to maintain a persistent expression of transgenes delivered by myoblast-mediated gene transfer.

Phagocytosis of opsonized red blood cells by hepatocytes after gene transfer of chimeric Fc γR III Aα and γcDNA

2001 ◽  
Vol 120 (5) ◽  
pp. A356-A357
Author(s):  
M FURUKAWA ◽  
Y MAGAMI ◽  
D NAKAYAMA ◽  
F MORIYASU ◽  
J PARK ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Red Blood Cells ◽  
Blood Cells

1860: Generation of Fluorescent Sperm by Use of in VIVO Gene Transfer to Hamster Testes

2007 ◽  
Vol 177 (4S) ◽  
pp. 617-618
Author(s):  
Hiraki Kubota ◽  
Kevin Coward ◽  
Olivia Hibbitt ◽  
Nilendran Prathalingam ◽  
William Holt ◽  
...  
Keyword(s):  
Gene Transfer ◽  
In Vivo Gene Transfer

1509: Adeno-Associated Virus-Mediated Human IL-10 Gene Transfer Suppresses the Development of Experimental Autoimmune Orchitis

2005 ◽  
Vol 173 (4S) ◽  
pp. 409-409
Author(s):  
Masami Watanabe ◽  
Atsushi Nagai ◽  
Norihiro Kusumi ◽  
Yasutomo Nasu ◽  
Hiromi Kumon ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Adeno Associated Virus ◽  
Autoimmune Orchitis ◽  
Experimental Autoimmune

1011: Suppression of Renal Cell Carcinoma Lung Metastasis by Adeno-Associated Virus (AAV) Mediated Gene Transfer of a Soluble Receptor of VEGF

2004 ◽  
Vol 171 (4S) ◽  
pp. 267-267
Author(s):  
Ichiro Yoshimura ◽  
Yasunori Mizuguchi ◽  
Akira Miyajima ◽  
Tomohiko Asano ◽  
Hiroaki Mizukami ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Gene Transfer ◽  
Cell Carcinoma ◽  
Lung Metastasis ◽  
Renal Cell ◽  
Soluble Receptor ◽  
Adeno Associated Virus
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