IGF-I Induces the Uncoupling Protein Gene Expression in Fetal Rat Brown Adipocyte Primary Cultures: Role of C/EBP Transcription Factors

1994 ◽  
Vol 201 (2) ◽  
pp. 813-819 ◽  
Author(s):  
C. Guerra ◽  
M. Benito ◽  
M. Fernandez
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Protein Gene ◽  
Uncoupling Protein ◽  
Primary Cultures ◽  
Brown Adipocyte ◽  
Fetal Rat ◽  
Igf I ◽  
Uncoupling Protein Gene

scholarly journals Triiodothyronine Induces the Transcription of the Uncoupling Protein Gene and Stabilizes Its mRNA in Fetal Rat Brown Adipocyte Primary Cultures

1996 ◽  
Vol 271 (4) ◽  
pp. 2076-2081 ◽  
Author(s):  
Carmen Guerra ◽  
Cesar Roncero ◽  
Almudena Porras ◽  
Margarita Fernández ◽  
Manuel Benito
Keyword(s):  
Protein Gene ◽  
Uncoupling Protein ◽  
Primary Cultures ◽  
Brown Adipocyte ◽  
Fetal Rat ◽  
Uncoupling Protein Gene

Influence of the cytoskeleton on surfactant protein gene expression in cultured rat alveolar type II cells

1998 ◽  
Vol 274 (1) ◽  
pp. L87-L96 ◽  
Author(s):  
John M. Shannon ◽  
Tianli Pan ◽  
Karen E. Edeen ◽  
Larry D. Nielsen
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Primary Cultures ◽  
Surfactant Protein ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Partial Recovery ◽  
Type Ii ◽  
Alveolar Type Ii Cells

We have investigated the role of the cytoskeleton in surfactant protein gene expression. Cytochalasin D (CD), colchicine (Col), or nocodazole (Noco) were tested on primary cultures of adult rat alveolar type II cells. Treatment with any of the drugs did not result in dramatic cell shape changes, but ultrastructural examination revealed that the cytoplasm of cells treated with CD was markedly disorganized; cells treated with Col did not exhibit such changes. Treatment with any of the three drugs resulted in a reduction in surfactant protein (SP) mRNAs. These decreases were not the result of cell toxicity, since overall protein synthesis was unimpaired by drug treatment. Washing the cells followed by an additional 2 days of culture resulted in a reaccumulation of SP mRNAs in CD-treated cells but not in Col-treated cells. Washing of Noco-treated cultures resulted in partial recovery. SP mRNA stability was estimated in the presence or absence of cytoskeleton-disrupting drugs. Disruption of either microfilaments or microtubules significantly affected the half-lives of mRNAs for SP-A, SP-B, and SP-C. These data support a role for the cytoskeleton in the maintenance of type II cell differentiation and suggest that the role of the cytoskeleton is at least in part to stabilize SP mRNAs.

Effect of Naltrexone on Feeding, Neuropeptide Y and Uncoupling Protein Gene Expression during Lactation

1997 ◽  
Vol 65 (4) ◽  
pp. 259-264 ◽  
Author(s):  
Catherine M. Kotz ◽  
Allen S. Levine ◽  
Charles J. Billington
Keyword(s):  
Gene Expression ◽  
Neuropeptide Y ◽  
Protein Gene ◽  
Uncoupling Protein ◽  
Uncoupling Protein Gene
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