Fluorescence Assay for the Binding of Ribonuclease A to the Ribonuclease Inhibitor Protein

2002 ◽  
Vol 306 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Richele L. Abel ◽  
Marcia C. Haigis ◽  
Chiwook Park ◽  
Ronald T. Raines
Keyword(s):  
Ribonuclease A ◽  
Fluorescence Assay ◽  
Inhibitor Protein ◽  
Ribonuclease Inhibitor

scholarly journals Theoretical treatment of tight-binding inhibition of an enzyme. Ribonuclease inhibitor as special case

1988 ◽  
Vol 253 (2) ◽  
pp. 517-522 ◽  
Author(s):  
J M Fominaya ◽  
J M García-Segura ◽  
M Ferreras ◽  
J G Gavilanes
Keyword(s):  
Tight Binding ◽  
Theoretical Treatment ◽  
Inhibitor Protein ◽  
Ribonuclease Inhibitor ◽  
Rat Testis ◽  
Binding Inhibition ◽  
Different Types ◽  
Functional Consequences ◽  
Inhibitor System ◽  
Special Case

A general treatment of very tight-binding inhibition is described. It was applied to purified endogenous RNAase inhibitor from rat testis. This treatment discriminates among the different types of inhibition and allows for calculation of the inhibition parameters. When very tight-binding inhibitions are studied at similar molar concentrations of both enzyme and inhibitor, a further approach is required. This is also described and applied to the RNAase inhibitor. A Ki value of 3.2 x 10(-12) M was found for this inhibitor protein. On the basis of this result, it was considered inappropriate to classify this type of inhibitor in terms of competitive or non-competitive, as has been done for such inhibitors so far. Functional consequences of this analysis are discussed for the RNAase-RNAase inhibitor system.

scholarly journals Inhibition of Human Pancreatic Ribonuclease by the Human Ribonuclease Inhibitor Protein

2007 ◽  
Vol 368 (2) ◽  
pp. 434-449 ◽  
Author(s):  
R. Jeremy Johnson ◽  
Jason G. McCoy ◽  
Craig A. Bingman ◽  
George N. Phillips ◽  
Ronald T. Raines
Keyword(s):  
Inhibitor Protein ◽  
Ribonuclease Inhibitor ◽  
Pancreatic Ribonuclease ◽  
Human Pancreatic Ribonuclease

scholarly journals Thiol-Disulfide Exchange of Ribonuclease Inhibitor Bound to Ribonuclease A EVIDENCE OF ACTIVE INHIBITOR-BOUND RIBONUCLEASE

1995 ◽  
Vol 270 (48) ◽  
pp. 28570-28578 ◽  
Author(s):  
Mercedes Ferreras ◽  
José G. Gavilanes ◽  
Carlos López-Otín ◽  
Juan M. García-Segura
Keyword(s):  
Ribonuclease A ◽  
Ribonuclease Inhibitor ◽  
Active Inhibitor ◽  
Disulfide Exchange
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