Analysis of Highly Polar Compounds of Plant Origin: Combination of Hydrophilic Interaction Chromatography and Electrospray Ion Trap Mass Spectrometry

2002 ◽  
Vol 301 (2) ◽  
pp. 298-307 ◽  
Author(s):  
Vladimir V. Tolstikov ◽  
Oliver Fiehn
Keyword(s):  
Mass Spectrometry ◽  
Ion Trap ◽  
Polar Compounds ◽  
Hydrophilic Interaction Chromatography ◽  
Ion Trap Mass Spectrometry ◽  
Plant Origin ◽  
Hydrophilic Interaction ◽  
Highly Polar Compounds

TRIGLYCERIDE COMPOSITION OF SIXTEEN STRAINS OF MARINE DIATOM

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lily M.G. Panggabean ◽  
Abdullah Rasyid ◽  
Zarrah Duniani ◽  
Yana Meliana ◽  
Indah Kurniasih
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Double Bond ◽  
Unsaturated Fatty Acid ◽  
Ion Trap ◽  
Carbon Chain ◽  
Highly Unsaturated Fatty Acid ◽  
Marine Diatom ◽  
Ion Trap Mass Spectrometry ◽  
Spectra Analysis

Trigliceride or triacylglicerol (TAG) composition in crude oil of sixteen strain of marine diatom has been detected by spectra analyses on an Electrospray - Ion Trap – Mass Spectrometry (ESI-IT-MS) HCT Bruker-Daltonic GmbH instrument with AgNO3 used as coordination ionization agent. Biomass samples of each microalga strain were taken from early and late stationary cultures in f/2 enriched seawater and algal oils were extracted according to Bligh and Dyer. Results from spectra analysis showed that P-Pt-P (C16:0-C16:1-C16:0) were distinguished in TAG from diatom strains Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.1, Thalasiossira sp.2, Thalasiossira sp.3, Navicula sp. 1, Navicula sp. 2, Navicula sp. 3, Navicula sp. 4, Nitzschia sp. 2 and Amphora sp. In contrast, TAGs in Melosira sp. included P-P-P (C16:0-C16:0-C16:0) and P-P-O (C16:0-C16:0-C18:1) were identified. TAGs from Chaetoceros sp. were the most varies among samples, i.e. P-Pt-P (C16:0-C16:1-C16:0), A-P-M (C20:4-C16:0-C14:0), P-Pt-Lt (C16:0-C16:1-C18:3), P-Pt-A (C16:0-C16:1-C20:4), D-P-P (C22:6-C16:0-C16:0), A-Ln-P (C20:4-C18:2-C16:0). Various TAGs were also detected in Nitzschia sp.2, i.e. P-Pt-M (C16:0-C16:1-C14:0), P-Pt-P (C16:0-C16:1-C16:0), P-Pt-S (C16:0-C16:1-C18:0), P-Pt-A (C16:0-C16:1-C20:4). TAGs composition in Skeletonema strains that similar to those in Nitzschia sp.1 has longer carbon, i.e. P-P-O (C16:0-C16:0-C18:1), P-O-O (C16:0-C18:1-C18:1) and O-O-O (C18:1-C18:1-C18:1). TAGs with longer carbon chain and more double bond including highly unsaturated fatty acid C20:4 were increased with culture age in diatoms Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.2, Navicula sp.1 and Nitzschia sp. 2.Keywords: diatom, TAG, ESI-IT-MS, f/2, early and late stationary

Electron spray ion trap mass spectrometry of isoflavones and isoflavone aglycones of Semen Sojae Praeparatum

2013 ◽  
Vol 33 (10) ◽  
pp. 1108-1115
Author(s):  
Gao FANG ◽  
Peng ZHANG ◽  
Xiao-lan YE ◽  
Xia ZHU ◽  
Xin ZHAO ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Trap ◽  
Ion Trap Mass Spectrometry
Sign in / Sign up

Export Citation Format

Share Document