High-Performance Liquid Chromatography Analysis of Molecular Species of Sphingolipid-Related Long Chain Bases and Long Chain Base Phosphates in Saccharomyces cerevisiae after Derivatization with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate

2001 ◽  
Vol 298 (2) ◽  
pp. 283-292 ◽  
Author(s):  
Robert L Lester ◽  
Robert C Dickson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Molecular Species ◽  
High Performance ◽  
Chain Base ◽  
Chromatography Analysis ◽  
Long Chain Base ◽  
Long Chain ◽  
Performance Liquid Chromatography Analysis

scholarly journals 5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA.

1984 ◽  
Vol 4 (5) ◽  
pp. 985-988 ◽  
Author(s):  
J H Proffitt ◽  
J R Davie ◽  
D Swinton ◽  
S Hattman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Restriction Enzyme ◽  
High Performance ◽  
Cytosine Methylation ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
Chromatography Analysis ◽  
Performance Liquid Chromatography Analysis

We examined the DNA of Saccharomyces cerevisiae by both HpaII-MspI restriction enzyme digestion and high-performance liquid chromatography analysis for the possible presence of 5-methylcytosine. Both of these methods failed to detect cytosine methylation within this yeast DNA; i.e., there is less than 1 5-methylcytosine per 3,100 to 6,000 cytosine residues.

5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA

1984 ◽  
Vol 4 (5) ◽  
pp. 985-988
Author(s):  
J H Proffitt ◽  
J R Davie ◽  
D Swinton ◽  
S Hattman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Restriction Enzyme ◽  
High Performance ◽  
Cytosine Methylation ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
Chromatography Analysis ◽  
Performance Liquid Chromatography Analysis

We examined the DNA of Saccharomyces cerevisiae by both HpaII-MspI restriction enzyme digestion and high-performance liquid chromatography analysis for the possible presence of 5-methylcytosine. Both of these methods failed to detect cytosine methylation within this yeast DNA; i.e., there is less than 1 5-methylcytosine per 3,100 to 6,000 cytosine residues.

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