One-Step Duplex Reverse Transcription–Polymerase Chain Reaction for Quantitative Assessment of RNA Degradation

2001 ◽  
Vol 295 (1) ◽  
pp. 113-116 ◽  
Author(s):  
Michio Sugita ◽  
Jerry L. Haney ◽  
Robert M. Gemmill ◽  
Wilbur A. Franklin
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Quantitative Assessment ◽  
Rna Degradation ◽  
Chain Reaction ◽  
One Step ◽  
Polymerase Chain

scholarly journals One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses

2016 ◽  
Vol 7 (3) ◽  
pp. 205-209 ◽  
Author(s):  
Sun-Whan Park ◽  
Ye-Ji Lee ◽  
Won-Ja Lee ◽  
Youngmee Jee ◽  
WooYoung Choi
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
One Step ◽  
Polymerase Chain

Detection ofPotato virus Yfrom the stylets of a single aphid by one-step reverse transcription polymerase chain reaction

2013 ◽  
Vol 147 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Jianhua Zhang ◽  
Xianzhou Nie ◽  
Upeksha Nanayakkara ◽  
Sébastien Boquel ◽  
Marie Andrée Giguère ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
One Step ◽  
Polymerase Chain

Analysis of the persistence time of the SARS-CoV-2 virus in the cadaver and the risk of passing infection to autopsy staff

2021 ◽  
pp. 002581722098060
Author(s):  
Isabella Aquila ◽  
Pietrantonio Ricci ◽  
Carlo Filippo Bonetta ◽  
Matteo Antonio Sacco ◽  
Federico Longhini ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Post Mortem ◽  
Forensic Pathologist ◽  
Chain Reaction ◽  
Sample Group ◽  
Persistence Time ◽  
One Step ◽  
Polymerase Chain

The activity of the SARS-CoV-2 virus has not yet been studied in a post-mortem setting. The absence of these data has led to the prohibition of exposure of infected corpses during burial procedures. Our aim was to assess the virus’s persistence and the possibility of transmission in the post-mortem phase including autopsy staff. The sample group included 29 patients who were admitted to our Covid-19 Centre who died during hospitalisation and the autopsy staff. All the swabs were subjected to a one-step real-time reverse transcription polymerase chain reaction with cycle threshold (Ct) values. Swab collection was performed at 2 h, 4 h, 6 h, 12 h, over 24 since death. The following were the analysis of patients’ swabs: 10 cases were positive 2 h after death; 10 cases positive 4 h after death; 9 cases were found positive 6 h after death; 7 cases positive 12 h after death; 9 cases remained positive 24 h after death. The swabs performed on all the forensic pathologist staff on duty who performed the autopsies were negative. The choice to avoid rituals and the display of corpses before and at the burial procedures given appears cautiously valid due to the persistence of the SARS-CoV-2 virus in the post-mortem period. Although the caution in choosing whether or not to perform an autopsy on infected corpses is acceptable, not to perform autopsies is not biologically supported.

A one-step quantitative reverse transcription polymerase chain reaction procedure

2001 ◽  
Vol 6 (3) ◽  
pp. 100-107 ◽  
Author(s):  
Kim L Kelleher ◽  
Kwong-Joo Leck ◽  
Ian A Hendry ◽  
Klaus I Matthaei
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Procedure ◽  
One Step ◽  
Polymerase Chain
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