Diphenylamine–Aniline–Phosphoric Acid Reagent, a Versatile Spray Reagent for Revealing Glycoconjugates on Thin-Layer Chromatography Plates

2000 ◽  
Vol 287 (2) ◽  
pp. 337-339 ◽  
Author(s):  
Kimberly Anderson ◽  
Su-Chen Li ◽  
Yu-Teh Li
Keyword(s):  
Phosphoric Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spray Reagent ◽  
Acid Reagent ◽  
Layer Chromatography

scholarly journals Quantification of Eugenol, Luteolin, Ursolic Acid, and Oleanolic Acid in Black (Krishna Tulasi) and Green (Sri Tulasi) Varieties of Ocimum sanctum Linn. Using High-Performance Thin-Layer Chromatography

2006 ◽  
Vol 89 (6) ◽  
pp. 1467-1474 ◽  
Author(s):  
Sheetal Anandjiwala ◽  
Jyoti Kalola ◽  
Mandapati Rajani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Ocimum Sanctum ◽  
Relative Standard ◽  
Recovery Study ◽  
Acid Reagent ◽  
Layer Chromatography

Abstract Ocimum sanctum (family Lamiaceae) is a reputed drug of Ayurveda, commonly known as Tulasi. Inthe present work, we quantified 4 marker compounds, viz., eugenol, luteolin, ursolic acid, and oleanolic acid, from the leaf of green and black varieties of O. sanctum using high-performance thin-layer chromatography (HPTLC) with densitometry. The methods were found to be precise, with relative standard deviation (RSD) values for intraday analyses in the range of 0.52 to 0.91%, 0.77 to 1.29%, 0.11 to 0.16%, and 0.34 to 0.42% and for interday analyses in the range of 0.73 to 0.96%, 1.02 to 2.08%, 0.11 to 0.12%, and 0.39 to 0.64% for different concentrations of eugenol, luteolin, ursolic acid, and oleanolic acid, respectively. Instrumental RSD values were 0.24, 0.39, 0.21, and 0.18% for eugenol, luteolin, ursolic acid, and oleanolic acid, respectively. Accuracy of the methods was checked by conducting a recovery study at 3 different levels for the 4 compounds, and the average recoveries were found to be 99.73, 99.3, 100.58, and 100.57%, respectively. Eugenol content ranged from 0.175 to 0.362% (w/w) and luteolin from 0.019 to 0.046% (w/w) in the samples analyzed. Green variety was found to contain higher amounts of ursolic acid [0.478 and 0.348% (w/w), from Sources 1 and 2, respectively] than the black variety [0.252 and 0.264% (w/w) from Sources 1 and 2, respectively]. Black variety had 0.174 and 0.218% (w/w) of oleanolic acid from Sources 1 and 2, respectively, while it was not detected in the green variety. Ursolic acid and oleanolic acid ran at the same Rf value and could not be resolved in several solvent systems tried. However, we observed that only ursolic acid gave yellow fluorescence under 366 nm ultraviolet light after derivatization with anisaldehydesulfuric acid reagent. The HPTLC-densitometry methods for the quantification of the 4 markers in O. sanctum leaf will have the applicability in quality control.

Spray reagent for the detection of amino acids on thin layer chromatography plates

Amino Acids ◽  
1993 ◽  
Vol 4 (1-2) ◽  
pp. 193-196 ◽  
Author(s):  
B. Basak ◽  
U. K. Bhattacharyya ◽  
S. Laskar
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spray Reagent ◽  
Layer Chromatography

Use of Fluorescamine ("Fluram") to Detect Amphetamine in Urine by Thin-Layer Chromatography

1974 ◽  
Vol 20 (2) ◽  
pp. 272-274 ◽  
Author(s):  
Bernard Klein ◽  
Joan E Sheehan ◽  
E Grunberg
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Drugs Of Abuse ◽  
Thin Layer Plate ◽  
Spray Reagent ◽  
Layer Chromatography

Abstract A "Fluram" (fluorescamine; 4-phenylspiro[furan-2(3H)-1'-phthalan]-3,3'-dione) spray reagent will detect as little as 250 ng of amphetamine in an extract of amphetamine-containing urine, after it has been separated by thin-layer chromatography. The fluorescence is stable for about 20 h and can be renewed by respraying. Fluram spray does not interfere with reagent sprays used to detect other drugs of abuse. Amphetamine on the thin-layer plate can be measured by extracting the fluorescent area of silica gel and measuring the fluorescence in a fluorimeter. Recovery of 1.0 µg and 2.0 µg of amphetamine averaged 69% and 67%, respectively.

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