A HPLC–Fluorescence Detection Method for Determination of Cardiac Phospholipase D Activity in Vitro

2000 ◽  
Vol 286 (2) ◽  
pp. 277-281 ◽  
Author(s):  
D. Kemken ◽  
K. Mier ◽  
H.A. Katus ◽  
G. Richardt ◽  
T. Kurz
Keyword(s):  
Fluorescence Detection ◽  
Phospholipase D ◽  
Detection Method

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

Assessment of a Semiquantitative Liquid Chromatography- Fluorescence Detection Method for the Determination of Paralytic Shellfish Poisoning Toxin Levels in Bivalve Molluscs from Great Britain

2014 ◽  
Vol 97 (2) ◽  
pp. 492-497 ◽  
Author(s):  
Andrew D Turner ◽  
Monika Dhanji-Rapkova ◽  
Clothilde Baker ◽  
Myriam Algoet
Keyword(s):  
Great Britain ◽  
Fluorescence Detection ◽  
Detection Method ◽  
Reference Method ◽  
Paralytic Shellfish Poisoning ◽  
Official Method ◽  
Shellfish Poisoning ◽  
Bivalve Molluscs ◽  
Paralytic Shellfish

Abstract AOAC Official Method 2005.06 precolumn oxidation LC-fluorescence detection method has been used for many years for the detection and quantitation of paralytic shellfish poisoning (PSP) toxins in bivalve molluscs. After extensive single- and multiple-laboratory validation, the method has been slowly gaining acceptance worldwide as a useful and practical tool for official control testing. In Great Britain, the method has become routine since 2008, with no requirement since then for reverting back to the bioassay reference method. Although the method has been refined to be semiautomated, faster, and more reproducible, the quantitation step can be complex and time-consuming. An alternative approach was developed to utilize the qualitative screening results for generatinga semiquantitative results assessment. Data obtained over 5 years enabled the comparison of semiquantitative and fully quantitative PSP results in over 15 000 shellfish samples comprising eight different species showed that the semiquantitative approach resulted in over-estimated paralytic shellfish toxin levels by an average factor close to two in comparison with the fully quantified levels. No temporal trends were observed in the data or relating to species type, with the exception of surf clams. The comparison suggested a semiquantitative threshold of 800 μg saxitoxin (STX) eq/kg should provide a safe limitfor the determination of samples to be forwarded to full quantitation. However, the decision was taken to halve this limit to include an additional safety factor of 2, resulting in the use of a semiquantitative threshold of 400 μg STX eq/kg. Implementation of the semiquantitative method into routine testing would result in a significant reduction in the numbers of samples requiring quantitation and have a positive impact on the overall turnaround of reported PSP results. The refined method would be appropriate for any monitoring laboratory faced with high throughput requirements.

A long-wavelength fluorescent probe for amino compounds and its application in the determination of aliphatic amines

2018 ◽  
Vol 10 (26) ◽  
pp. 3188-3196
Author(s):  
Li-Jun Xia ◽  
Xiao-Feng Guo ◽  
Yan Ji ◽  
Liu Chen ◽  
Hong Wang
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Detection ◽  
Detection Method ◽  
Aliphatic Amines ◽  
Long Wavelength ◽  
Amino Compounds

In this study, a simple HPLC-fluorescence detection method for aliphatic amines was developed with a novel long-wavelength BODIPY-based derivatization reagent.

Determination of Abamectin Residues in Edible Oils by High-Performance Liquid Chromatography with Fluorescence Detection

2014 ◽  
Vol 97 (3) ◽  
pp. 928-932 ◽  
Author(s):  
Xue-Xue Miao ◽  
Yuan-Yuan Yang ◽  
Xiao-Yun Yang ◽  
Qi-Liang Huang ◽  
Hai Hong
Keyword(s):  
High Performance Liquid Chromatography ◽  
Fluorescence Detection ◽  
Mobile Phase ◽  
High Performance ◽  
Detection Method ◽  
Edible Oils ◽  
Edible Oil ◽  
Time Saving ◽  
Satisfactory Precision

Abstract A rapid and simple HPLC-fluorescence detection method has been developed for the determination of abamectin residues in edible oil. Residues are extracted with acetonitrile and by vortexing and then directly derivatized with no need for a time-consuming cleanup step. Trifluoroacetic anhydride and N-methylimidazole were used as derivatizing agents of abamectin. Abamectin was detected and quantitated with fluorescence detection (excitation: 365 nm; emission: 475 nm), and methanol was used as the mobile phase. The LOD was 0.001 mg/kg and the LOQ was 0.003 mg/kg. The recoveriesranged from 86 to 100.4% with satisfactory precision (RSD < 10.1%). This method proved to be sensitive, environmentally friendly, time-saving, and efficient.

A HPLC with fluorescence detection method for the determination of tetracyclines residues and evaluation of their stability in honey

Food Control ◽  
2010 ◽  
Vol 21 (5) ◽  
pp. 620-625 ◽  
Author(s):  
Gustavo Tayar Peres ◽  
Susanne Rath ◽  
Felix Guillermo Reyes Reyes
Keyword(s):  
Fluorescence Detection ◽  
Detection Method

Determination of Methoxamine in Human Plasma and Urine by a Validated HPLC-Fluorescence Detection Method

2013 ◽  
Vol 96 (5) ◽  
pp. 987-990
Author(s):  
Sevgi Tatar Ulu
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
Detection Method ◽  
Hplc Method ◽  
Fluorescent Product ◽  
Hplc Separation ◽  
Human Plasma And Urine ◽  
System Suitability ◽  
Isocratic Hplc

Abstract A sensitive HPLC method for the determination of methoxamine in human plasma and urine is described. The method is based on derivatization of methoxamine with 4-chloro-7-nitrobenzofurazan in borate buffer of pH 9.5 to yield a yellow, fluorescent product. Isocratic HPLC separation was achieved on an Inertsil C18 column (250 × 4.6 mm id, 5 μm particle size) using the mobile phase methanol–water (60 + 40, v/v) at a flow rate of 1.2 mL/min. Fluorescence detection was used at excitation and emission wavelengths of 458 and 521 nm, respectively. The assay was linear over the concentration ranges of 10–250 and 20–300 ng/mL for plasma and urine, respectively. The LOD values were 3.3 and 6.8 ng/mL and the LOQ values were 10 and 20 ng/mL for plasma and urine, respectively. The extraction recoveries were more than 97.10%. After strict validation, the method indicated good performance in terms of linearity, sensitivity, precision, accuracy (recovery), robustness, and system suitability, and it was successfully applied to the determination of methoxamine in human plasma and urine.

Determination of gibberellins using HPLC coupled with fluorescence detection

2016 ◽  
Vol 8 (7) ◽  
pp. 1520-1526 ◽  
Author(s):  
Yin-Hua Lu ◽  
Yan-Ming Cao ◽  
Xiao-Feng Guo ◽  
Hong Wang ◽  
Hua-Shan Zhang
Keyword(s):  
Fluorescence Detection ◽  
Detection Method ◽  
Plant Tissues ◽  
Precolumn Derivatization

In this work, a HPLC-fluorescence detection method for the determination of GAs in plant tissues and foods has been reported using TMBB-EDAN as a precolumn derivatization reagent.

HPLC-fluorescence detection method for determination of key intermediates of the lincomycin biosynthesis in fermentation broth

2009 ◽  
Vol 393 (6-7) ◽  
pp. 1779-1787 ◽  
Author(s):  
Zdeněk Kameník ◽  
Jan Kopecký ◽  
Markéta Marečková ◽  
Dana Ulanová ◽  
Jitka Novotná ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Detection Method ◽  
Fermentation Broth
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