A High-Performance Liquid Chromatographic Method to Measure Sphingosine 1-Phosphate and Related Compounds from Sphingosine Kinase Assays and Other Biological Samples

2000 ◽  
Vol 281 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Thomas B. Caligan ◽  
Katherine Peters ◽  
Joyce Ou ◽  
Elaine Wang ◽  
Julie Saba ◽  
...  
Keyword(s):  
Biological Samples ◽  
High Performance ◽  
Chromatographic Method ◽  
Sphingosine Kinase ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Sphingosine 1 Phosphate ◽  
Liquid Chromatographic ◽  
Related Compounds

scholarly journals An Isocratic Reversed-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Benzoyl Peroxide and the Related Compounds Benzoic Acid, Benzaldehyde, Ethyl Benzoate, Methylparaben, and Propylparaben in Dermal Preparations

2008 ◽  
Vol 91 (5) ◽  
pp. 1025-1036 ◽  
Author(s):  
Mohammed Shahid Ali ◽  
Syed Rafiuddin ◽  
Muhammad Imran Munir ◽  
Mohsin Ghori ◽  
Aamer Roshanali Khatri
Keyword(s):  
Benzoic Acid ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Ethyl Benzoate ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Related Compounds

Abstract A reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of benzoyl peroxide and the related compounds benzoic acid (BA), methylparaben, benzaldehyde, propylparaben, and ethyl benzoate. The compounds are separated on a column containing octadecyl silane chemically bonded to porous silica particles. The mobile phase is acetonitrilebuffer (45 + 55, v/v). Solutions are injected into the chromatographic system under isocratic conditions at a constant flow rate of 1.5 mL/min with UV detection at 235 nm. Analysis of stability samples showed rapid accumulation of BA by thermal degradation. A rationale has been established for the acceptable limit of BA in the formulation, which already contains BA (0.2) as a preservative. The proposed method is efficient and determines the active compound and 5 related compounds in a run time of 20 min. The method was validated according to the guidelines of the International Conference on Harmonization and demonstrated good agreement with the validation requirements.

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