Quantification of Glutamine in Proteins and Peptides Using Enzymatic Hydrolysis and Reverse-Phase High-Performance Liquid Chromatography

1999 ◽  
Vol 269 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Marlene Tsao ◽  
Don E. Otter
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
High Performance ◽  
Reverse Phase ◽  
Proteins And Peptides

L-to-D-peptide isomerase in male echidna venom

2010 ◽  
Vol 58 (5) ◽  
pp. 284 ◽  
Author(s):  
Jennifer M. S. Koh ◽  
Leesa Haynes ◽  
Katherine Belov ◽  
Philip W. Kuchel
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hind Limb ◽  
Reproductive Behaviour ◽  
Reverse Phase ◽  
Isomerase Activity ◽  
Rp Hplc ◽  
Synthetic Hexapeptide ◽  
Proteins And Peptides

The monotremes (the echidnas and the platypus) display both mammalian and reptilian features. Male monotremes have a bilateral crural gland that is connected via a duct to a spur on each hind limb. Male echidnas appear not to use their spurs as weapons in aggressive acts, but the crural system may have a role in reproductive behaviour because it appears only to be active during the breeding season. The secretions produced by the echidna’s crural gland have not hitherto been biochemically or pharmacologically characterised. We used reverse-phase high-performance liquid chromatography (RP-HPLC) to separate the components of echidna venom and compared the chromatograms with those from platypus venom. The echidna venom appears to contain fewer proteins and peptides than platypus venom; however, it appears to have defensin-like peptides that behave similarly on RP-HPLC to those in platypus venom. Like platypus venom, echidna venom has peptidyl aminoacyl l/d-peptide isomerase activity. An RP-HPLC-based assay showed that the second amino acid residue, of a probe synthetic hexapeptide, was converted into the d-form, when incubated with echidna venom.

Separation and purification of individual neurofilament proteins by reverse-phase high-performance liquid chromatography

1986 ◽  
Vol 153 (2) ◽  
pp. 230-234 ◽  
Author(s):  
Koon-Sea Hui ◽  
Maria Hui ◽  
Fung-Chow Chiu ◽  
Miriam Banay-Schwartz ◽  
Teresita Deguzman ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Separation And Purification ◽  
Neurofilament Proteins ◽  
Reverse Phase
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