Bovine Serum Albumin–(7-Hydroxycoumarin-4-acetic Acid) Complex: Applications to the Fluorometric Measurement of Fatty Acid Concentrations

1999 ◽  
Vol 267 (2) ◽  
pp. 366-372 ◽  
Author(s):  
Erland J.F. Demant
Keyword(s):  
Acetic Acid ◽  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Acid Complex

scholarly journals Hypothyroidism in rats does not lower mitochondrial ADP/O and H+/O ratios

1988 ◽  
Vol 250 (2) ◽  
pp. 477-484 ◽  
Author(s):  
R P Hafner ◽  
M D Brand
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adenylate Kinase ◽  
Bovine Serum ◽  
Mitochondrial Protein ◽  
Experimental Conditions ◽  
Low Concentrations ◽  
The Difference

We investigated reports that mitochondria isolated from hypothyroid rats have decreased ADP/O and H+/O ratios. We observed no decrease in the H+/O ratio in mitochondria from hypothyroid rats, in the presence of either 2% (w/v) fatty-acid-free bovine serum albumin or 100 nM free Ca2+. The ADP/O ratio in mitochondria isolated from hypothyroid rats in the presence of 2% fatty-acid-free bovine serum albumin was measured. Under normal experimental conditions we found no decrease in the ADP/O ratio, relative to that measured for littermate controls. At the low concentrations of mitochondrial protein used in the previously reported studies, the ADP/O ratio of mitochondria from hypothyroid rats was decreased, whereas that for control rats was only slightly decreased. The difference between the ADP/O ratios measured for mitochondria form hypothyroid rats and from control rats under these conditions was eliminated by inhibition of endogenous adenylate kinase. We suggest that the lowering of the apparent ADP/O ratio in mitochondria from hypothyroid rats at low concentrations of mitochondrial protein is an experimental artefact resulting from the breakdown of ADP to AMP.

Binding of bis(p-chlorophenyl) acetic acid with bovine serum albumin

1976 ◽  
Vol 6 (2) ◽  
pp. 170-174 ◽  
Author(s):  
R. Haque ◽  
J. Deagen ◽  
F.J. Biros ◽  
R.T. Ross
Keyword(s):  
Acetic Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

Binding of some long-chain fatty acid anions and alcohols by bovine serum albumin

Biochemistry ◽  
1968 ◽  
Vol 7 (4) ◽  
pp. 1357-1361 ◽  
Author(s):  
Jacqueline A. Reynolds ◽  
Sarah. Herbert ◽  
Jacinto. Steinhardt
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Long Chain

Investigations of acetaminophen binding to bovine serum albumin in the presence of fatty acid: Fluorescence and 1H NMR studies

2009 ◽  
Vol 924-926 ◽  
pp. 332-337 ◽  
Author(s):  
B. Bojko ◽  
A. Sułkowska ◽  
M. Maciążek-Jurczyk ◽  
J. Równicka ◽  
W.W. Sułkowski
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
1H Nmr ◽  
Bovine Serum ◽  
Nmr Studies
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