Use of a Fluorescence Plate Reader for Measuring Kinetic Parameters with Inner Filter Effect Correction

1999 ◽  
Vol 267 (2) ◽  
pp. 331-335 ◽  
Author(s):  
Yaya Liu ◽  
Warren Kati ◽  
Chih-Ming Chen ◽  
Rakesh Tripathi ◽  
Akhter Molla ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Inner Filter Effect ◽  
Filter Effect ◽  
Plate Reader ◽  
Fluorescence Plate Reader

Polyethyleneimine-functionalized carbon dots as a fluorescent probe for doxorubicin hydrochloride by an inner filter effect

2021 ◽  
Vol 112 ◽  
pp. 110743
Author(s):  
Min Yang ◽  
Yujia Yan ◽  
Enzhou Liu ◽  
Xiaoyun Hu ◽  
Hong Hao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Inner Filter Effect ◽  
Doxorubicin Hydrochloride ◽  
Filter Effect

Fluorometric discrimination of tyrosine isomers based on the inner filter effect of chiral Au nanoparticles on MoS2 quantum dots

2021 ◽  
Author(s):  
Qianqian Zhao ◽  
Datong Wu ◽  
Zhengzhi Yin ◽  
Wenrong Cai ◽  
Haifeng Zhou ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Au Nanoparticles ◽  
Inner Filter Effect ◽  
Filter Effect

A fluorescent chiral sensor is proposed based on the inner filter effect (IFE) of chiral Au nanoparticles (AuNPs) on MoS2 quantum dots (MoS2 QDs), which can be used for the...

scholarly journals An ultrasensitive colorimetric and fluorescence dual-readout assay for glutathione with a carbon dot–MnO2 nanosheet platform based on the inner filter effect

RSC Advances ◽  
2021 ◽  
Vol 11 (34) ◽  
pp. 21137-21144
Author(s):  
Mengyuan He ◽  
Ning Shang ◽  
Bo Zheng ◽  
Gege Yue
Keyword(s):  
Human Body ◽  
Body Fluid ◽  
Inner Filter Effect ◽  
Carbon Dot ◽  
Filter Effect ◽  
Human Body Fluid ◽  
Mno2 Nanosheet

An ultrasensitive colorimetric and fluorescence dual-readout assay based on carbon dot–MnO2 nanosheets platform was developed for GSH detection in human body fluid samples.

A silicon nanoparticle based turn off fluorescent sensor for sudan I

2016 ◽  
Vol 8 (28) ◽  
pp. 5701-5706 ◽  
Author(s):  
Ammu Rosin Jose ◽  
Unni Sivasankaran ◽  
Shalini Menon ◽  
Krishnapillai Girish Kumar
Keyword(s):  
Fluorescent Sensor ◽  
Inner Filter Effect ◽  
Silicon Nanoparticle ◽  
Filter Effect ◽  
Sudan I

A turn off sensor for sudan I based on fluorescent Si NPs which works via an inner filter effect (IFE).

scholarly journals Investigating the Size-Dependent Binding of Pristine nC60 to Bovine Serum Albumin by Multi-Spectroscopic Techniques

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 298
Author(s):  
Shufang Liu ◽  
Shu’e Wang ◽  
Zhanzuo Liu
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Binding Constants ◽  
Inner Filter Effect ◽  
Spectroscopic Techniques ◽  
Size Distributions ◽  
Size Dependent ◽  
Filter Effect ◽  
Vis Spectroscopy

The morphology of nanomaterials may affect their interaction with biomacromolecules such as proteins. Previous work has studied the size-dependent binding of pristine nC60 to bovine/human serum albumin using the fluorometric method and found that the fluorescence inner filter effect might affect this interaction. However, if it is necessary to accurately calculate and obtain binding information, the fluorescence inner filter effect should not be ignored. This work aimed to further investigate the effect of the fluorescence inner filter on the interaction between pristine nC60 with different particle sizes (140–160, 120–140, 90–110, 50–70, and 30–50 nm) and bovine serum albumin for a more accurate comprehension of the binding of pristine nC60 to bovine serum albumin. The nC60 nanoparticles with different size distributions used in the experiments were obtained by the solvent displacement and centrifugation method. UV-Vis spectroscopy and fluorescence spectroscopy were used to study the binding of nC60 with different size distributions to bovine serum albumin (BSA) before and after eliminating the fluorescence inner filter effect. The results showed that the fluorescence inner filter effect had an influence on the interaction between nC60 and proteins to some extent, and still did not change the rule of the size-dependent binding of nC60 nanoparticles to BSA. Further studies on the binding parameters (binding constants and the number of binding sites) between them were performed, and the effect of the binding on BSA structures and conformation were also speculated.

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