Effect of Vanadate on Different Forms of Coomassie Brilliant Blue and Protein Assay

1998 ◽  
Vol 258 (1) ◽  
pp. 143-146 ◽  
Author(s):  
Mohammed Momin Shareef ◽  
K.Taranath Shetty
Keyword(s):  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Protein Assay ◽  
Coomassie Brilliant

Study of Protein Adsorption and Conformational Change on DLC and Ti Surfaces Using FTIR and Coomassie Brilliant Blue Protein Assay

2007 ◽  
Vol 330-332 ◽  
pp. 893-896 ◽  
Author(s):  
Huang Yan ◽  
Xiao Ying Lü ◽  
Nan Huang
Keyword(s):  
Secondary Structure ◽  
Structure Analysis ◽  
Brilliant Blue ◽  
Material Surface ◽  
Coomassie Brilliant Blue ◽  
Protein Assay ◽  
Human Plasma Protein ◽  
Secondary Structure Analysis ◽  
Adsorbed Proteins ◽  
Coomassie Brilliant

The aim of present work was to study the interaction between human plasma protein-albumin (Alb) and immunoglobulin G (IgG) and the surfaces of two kinds of diamond-like carbon (DLC-A and DLC-B) and titanium (Ti) film. Fourier transform infrared spectroscopy (FTIR) was used to perform both quantity investigation and secondary structure analysis of above two proteins adsorbed on material surfaces. A modified Coomassie brilliant blue (CBB) protein assay was also used to study the amount of adsorbed proteins. The result of FTIR quantitative evaluation shows that the ratio of adsorbed Alb to IgG (RA/I) on three kinds of material surface has an order: DLC-A > DLC-B > Ti, which is coincide with the result from CBB protein assay. The result of secondary structure analysis shows that the conformation of Alb and IgG changes in a largest degree after adsorbed on Ti and a smallest degree on DLC-A surface. Both the results indicate that the anti-thrombogenicity of DLC-A seems to be the best and Ti is the worst.

A Mathematical Model for the Description of the Coomassie Brilliant Blue Protein Assay

1996 ◽  
Vol 233 (2) ◽  
pp. 160-168 ◽  
Author(s):  
B.A. Atherton ◽  
E.L. Cunningham ◽  
A.G. Splittgerber
Keyword(s):  
Mathematical Model ◽  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Protein Assay ◽  
Coomassie Brilliant
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