Separation of Phosphopeptides from Their Nonphosphorylated Forms by Reversed-Phase POROS Perfusion Chromatography at Alkaline pH

1997 ◽  
Vol 251 (1) ◽  
pp. 116-119 ◽  
Author(s):  
Hiroyuki Matsumoto ◽  
Esther S. Kahn ◽  
Naoka Komori
Keyword(s):  
Reversed Phase ◽  
Alkaline Ph ◽  
Perfusion Chromatography

scholarly journals Assay of Biogenic Amines Involved in Fish Decomposition

1996 ◽  
Vol 79 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Pierre Malle ◽  
Michel Vallé ◽  
Stephane Bouquelet
Keyword(s):  
Quantitative Determination ◽  
Quality Index ◽  
Reversed Phase ◽  
Alkaline Ph ◽  
Specific Criterion ◽  
Species Specific ◽  
Liquid Chromatographic ◽  
Phase Column ◽  
Reversed Phase Column

Abstract A liquid chromatographic (LC) method is described for quantitative determination of putrefaction amines: putrescine, cadaverine, histamine, spermidine, and spermine. These amines are extracted from fish bygrinding with perchloric acid at -20°C. The amines are reacted with dansyl chloride at alkaline pH, dried under a stream of nitrogen, placed in an automatic injector at -20°C, and separated ina Kromasil C18 reversed-phase column at 25°C. A new gradient elutes the amines in 22 min and regenerates the column in 30 min. Regression lines are obtained with high coefficients of correlation. This method is faster and more reproducible than other methods and shows that the quality index is a reliable, albeit species-specific, criterion of fish decomposition.

Quantitation of urinary normetanephrine and metanephrine by reversed-phase extraction and mass-fragmentographic analysis.

1982 ◽  
Vol 28 (1) ◽  
pp. 25-28 ◽  
Author(s):  
C Canfell ◽  
S R Binder ◽  
H Khayam-Bashi
Keyword(s):  
Mass Spectrometer ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Alkaline Ph ◽  
Gas Chromatograph ◽  
Standard Curve ◽  
Potential Value ◽  
Analytical Recovery ◽  
Urine Concentrations ◽  
Urine Specimens

Abstract Hydrolyzed urine with added ring-trideuterated normetanephrine and metanephrine is applied to wet C18-reversed-phase minicolumns. The "metanephrines" are eluted, dried, derivatized with pentafluoropropionic anhydride, and analyzed with the gas chromatograph-mass spectrometer. Ions for the nondeuterated and trideuterated compounds are monitored at ml z 458 and 461, respectively. For both normetanephrine and metanephrine, the standard curve is linear over the range 10-2000 micrograms/L and the procedure has adequate precision both within-run (CV less than 3%) and between-day (CV less than 7%). Alkaline pH in the extraction is important for optimal analytical recovery. We have examined the potential value of untimed urine specimens for screening purposes and compared 24-h urine concentrations of these analyses in normotensive and hypertensive persons.

Perfusion Chromatography on Reversed-Phase Column Allows Fast Analysis of Human Globin Chains

1996 ◽  
Vol 237 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Henri Wajcman ◽  
Rolande Ducrocq ◽  
Jean Riou ◽  
Mireille Mathis ◽  
Christian Godart ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Fast Analysis ◽  
Globin Chains ◽  
Perfusion Chromatography ◽  
Phase Column ◽  
Reversed Phase Column

Hochdruckflüssigkeitschromatographische Qualitätskontrolle einiger auf dem Markt befindlicher 123J/125J/131J/-Orthojodhippurane

1978 ◽  
Vol 17 (06) ◽  
pp. 283-287
Author(s):  
W. Bögl ◽  
K. Stockhausen
Keyword(s):  
Reversed Phase

Einige auf dem Markt befindliche 123J/125J/131J/-Orthojodhippurane wurden auf ihre radiochemische Reinheit untersucht. Als Analysenmethode wurde wegen ihrer großen Empfindlichkeit die Reversed-Phase-Hochdruckflüssigkeits-chromatographie ausgewählt. Die durch das als radiochemische Verunreinigung vorhandene freie Jodid bedingte unnötige Strahlenexposition von Schilddrüse, Ovarien, Testes und rotem Knochenmark wurde abgeschätzt. Alle untersuchten Or-thojodhippurane erfüllen die im Europäischen Arzneibuch festgelegten Mindestanforderungen an die radiochemische Reinheit.

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