An Enzyme-Linked Immunosorbent Assay for Detecting Proteolytic Activity of Hepatitis C Virus Proteinase

1997 ◽  
Vol 247 (2) ◽  
pp. 242-246 ◽  
Author(s):  
Norisue Takeshita ◽  
Nobuko Kakiuchi ◽  
Tsutomu Kanazawa ◽  
Yasumasa Komoda ◽  
Makoto Nishizawa ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Proteolytic Activity ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals High Frequency of False-Positive Hepatitis C Virus Enzyme-Linked Immunosorbent Assay in Rakai, Uganda

2013 ◽  
Vol 57 (12) ◽  
pp. 1747-1750 ◽  
Author(s):  
C. E. Mullis ◽  
O. Laeyendecker ◽  
S. J. Reynolds ◽  
P. Ocama ◽  
J. Quinn ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immunosorbent Assay ◽  
False Positive ◽  
High Frequency ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Frequency of Hepatitis C Virus in Diagnosed Tuberculosis Cases

2021 ◽  
Vol 15 (5) ◽  
pp. 1041-1043
Author(s):  
Muhammad Farooq Baig ◽  
Geeta Kumari ◽  
Amir Bux Detho ◽  
Abdul Saleem Qadeer ◽  
Ball Chand ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immunosorbent Assay ◽  
Rapid Test ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis C Virus Infection ◽  
Chain Reaction ◽  
Polymerase Chain

Background: The frequency of hepatitis C virus infection along with tuberculosis has not been widely investigated and very low statistics on rates of hepatitis C virus co-infection in tuberculosis patients. Hepatotoxicity is the major side effect of anti-tuberculosis therapy hepatitis HCVliver disease elevates the chances of hepatotoxicity up-to five folds. Aim: To see the frequency of Hepatitis Cvirus infection amongst people with diagnosed Tuberculosis using gene X-pert technique. To evaluate the factors associated with HCVinfection in patients with MTBtuberculosis and to determine sensitivity and specificity of the tests. Study design: Comparative analytical study. Place and duration of study: Pathology Department, Civil Hospital Mirpur Khas Sindh from 1stJanuary 2017 to 31st December 2018. Methodology: Three hundred and thirteen patients of tuberculosis diagnosed by Genexpert included while testing hepatitis C virus using immunochromotography rapid test technique, enzyme linked immunosorbent assay method and polymerase chain reaction test for confirmation. Results:Higher frequency of tuberculosis infection in males 57.8%, 42.5% between 20-39 years and 22% of hepatitis C virus infection in tuberculosis patients.Sensitivity of rapid test and enzyme-linked immunosorbent assay was 79% and 96% respectively while the specificity of rapid test and enzyme-linked immunosorbent assay was 91% and 99% respectively. Conclusion: The high frequency of hepatitis C virus co-infection was found among tuberculosis cases in Mirpur Khas Division Sindh. Enzyme-linked immunosorbent assay method is more accurate, reliable as compared to rapid immunochromatographytest for hepatitis C virus and polymerase chain reaction is still gold standard. Keywords: TB, Hepatitis C virus, Mycobacterium tuberculosis,, PCR, Genexpert, Rapid test

Sign in / Sign up

Export Citation Format

Share Document