Catalytic Hydrogenation of Leukotriene B4Enhances Sensitivity and Specificity of Gas Chromatography–Tandem Mass Spectrometry Techniques and Enables Simultaneous Analysis with Cysteinyl Leukotrienes in Biological Fluids

1996 ◽  
Vol 236 (1) ◽  
pp. 175-178 ◽  
Author(s):  
Dimitrios Tsikas ◽  
Joachim Fauler ◽  
Rubén D. Velásquez ◽  
Frank-Mathias Gutzki ◽  
Jürgen C. Frölich
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Tandem Mass Spectrometry ◽  
Sensitivity And Specificity ◽  
Catalytic Hydrogenation ◽  
Biological Fluids ◽  
Simultaneous Analysis ◽  
Tandem Mass ◽  
Cysteinyl Leukotrienes ◽  
Chromatography Tandem Mass Spectrometry

scholarly journals ANALYsis oF RYRRoLIDiNopHENoNE DERivATivEs iN BioLoGiOAL FLuiDs

2019 ◽  
Vol 19 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
IV. V Synbulatov ◽  
A. V Voronin ◽  
T. V Voronina
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Biological Fluids ◽  
Gas Chromatography Mass Spectrometry ◽  
Tandem Mass ◽  
Primary Metabolites ◽  
Chromatography Tandem Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Pyrrolidinophenone derivatives are the group of narcotic drugs controlled in the Russian Federation. The review presents the trends of biotransformation of а-pyrrolidinovalerophenone and 3,4-methylenedioxypyrovalerone, the data about their primary metabolites is provided. Various techniques of the sample preparation of biological fluids for analytical toxicology studies for substances of the pyrrolidinophenone derivative group are discussed. The use of enzymatic hydrolysis followed by solid-phase extraction (sorption) provides low detection limits for native sub- PHARMACY ФАРМАЦИЯ stances of this group and primary metabolites using small volumes of biological fluids (0.5 and 1.0 ng/ml blood for a-pyrrolidinovalerophenone and 3,4-methylenedioxypirovalonone respectively). The main characteristics of pyrro-lidinophenone derivatives (Kovac’s retention indices in nonpolar stationary liquid phases and the main characteristic ions in the mass spectra of electron impact) are presented. They allow to identify pyrrolidinophenone derivatives and their primary metabolites in biological fluids during chromatographic-mass spectrometric screening. Analytical possibilities of an alternative variant of screening for biological fluids i.e. analysis by using current immunochemical test systems, including “biochips” are discussed. The main methods of reliable identification and quantitative determination of pyrrolidinophenone derivatives are chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry. The detection limit of 3,4-methylenedioxypyralovalone in blood by gas chromatography-mass spectrometry is 1.0 ng/ml. The ranges of the determined concentrations of the method of quantitation by gas chromatography-mass spectrometry are 2.0-2000.0 ng/ml for blood and 0.05-50.0 ng/10 mm for hair. The high-performance chromatography-tandem mass spectrometry method with a triple quadrupole in the monitoring mode of multiple molecular reactions makes it possible to achieve a nearly complete suppression of analytical background “noise” for a sample, and to obtain detection and quantification limits for 3,4-methylenedioxypy-raloperone in cadaveric blood at a level of 10.0-100.0 pg/ml and 1.0-10.0 ng/ml, respectively. One of the advantages of the high-performance chromatography-tandem mass spectrometry system is the screening possibility.

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