Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 ◽  
Vol 162 (1) ◽  
pp. 156-159 ◽  
Author(s):  
P CHOMZYNSKI
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Chloroform Extraction ◽  
Guanidinium Thiocyanate

scholarly journals A single-step method for RNA isolation from tropical crops in the field

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
J.-C. Breitler ◽  
C. Campa ◽  
F. Georget ◽  
B. Bertrand ◽  
H. Etienne
Keyword(s):  
Rna Isolation ◽  
Single Step ◽  
Step Method ◽  
Tropical Crops

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

1983 ◽  
Vol 49 (01) ◽  
pp. 024-027 ◽  
Author(s):  
David Vetterlein ◽  
Gary J Calton
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Culture Media ◽  
Biological Fluids ◽  
Single Step ◽  
High Yield ◽  
Step Method ◽  
Commercial Preparation ◽  
E Coli ◽  
Tissue Culture Media

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

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