Structural Consequences of Divalent Metal Binding by the Adenylyl Cyclase Toxin of Bordetella pertussis

2001 ◽  
Vol 395 (2) ◽  
pp. 169-176 ◽  
Author(s):  
Christopher R. Rhodes ◽  
Mary C. Gray ◽  
Justin M. Watson ◽  
Tara L. Muratore ◽  
Sharon B. Kim ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Bordetella Pertussis ◽  
Metal Binding ◽  
Divalent Metal

scholarly journals Invasive adenylyl cyclase of Bordetella pertussis. Physical, catalytic, and toxic properties.

1990 ◽  
Vol 265 (18) ◽  
pp. 10686-10692
Author(s):  
F Gentile ◽  
L G Knipling ◽  
D L Sackett ◽  
J Wolff
Keyword(s):  
Adenylyl Cyclase ◽  
Bordetella Pertussis

scholarly journals Regulation of divalent metal ions to the aggregation and membrane damage of human islet amyloid polypeptide oligomers

RSC Advances ◽  
2021 ◽  
Vol 11 (21) ◽  
pp. 12815-12825
Author(s):  
Yajie Wang ◽  
Feihong Meng ◽  
Tong Lu ◽  
Chunyun Wang ◽  
Fei Li
Keyword(s):  
Metal Ions ◽  
Metal Binding ◽  
Membrane Damage ◽  
Islet Amyloid Polypeptide ◽  
Divalent Metal ◽  
Human Islet ◽  
Divalent Metal Ions ◽  
Islet Amyloid ◽  
Induced Membrane ◽  
Human Islet Amyloid Polypeptide

Their is a counteraction between a decrease in the disruptive ability of metal-associated oligomer species and an increase in the quantity of oligomers promoted by the metal binding in the activity of hIAPP induced membrane damage.

scholarly journals HK97 gp74 Possesses an α-Helical Insertion in the ββα Fold That Affects Its Metal Binding, cos Site Digestion, and In Vivo Activities

2020 ◽  
Vol 202 (8) ◽  
Author(s):  
Sasha A. Weiditch ◽  
Sarah C. Bickers ◽  
Diane Bona ◽  
Karen L. Maxwell ◽  
Voula Kanelis
Keyword(s):  
Metal Binding ◽  
Metal Ion ◽  
Divalent Metal ◽  
Metal Ion Binding ◽  
Divalent Metal Ions ◽  
Nmr Studies ◽  
Phage Dna ◽  
Divalent Metal Ion ◽  
Phage Morphogenesis

ABSTRACT The last gene in the genome of the bacteriophage HK97 encodes gp74, an HNH endonuclease. HNH motifs contain two conserved His residues and an invariant Asn residue, and they adopt a ββα structure. gp74 is essential for phage head morphogenesis, likely because gp74 enhances the specific endonuclease activity of the HK97 terminase complex. Notably, the ability of gp74 to enhance the terminase-mediated cleavage of the phage cos site requires an intact HNH motif in gp74. Mutation of H82, the conserved metal-binding His residue in the HNH motif, to Ala abrogates gp74-mediated stimulation of terminase activity. Here, we present nuclear magnetic resonance (NMR) studies demonstrating that gp74 contains an α-helical insertion in the Ω-loop, which connects the two β-strands of the ββα fold, and a disordered C-terminal tail. NMR data indicate that the Ω-loop insert makes contacts to the ββα fold and influences the ability of gp74 to bind divalent metal ions. Further, the Ω-loop insert and C-terminal tail contribute to gp74-mediated DNA digestion and to gp74 activity in phage morphogenesis. The data presented here enrich our molecular-level understanding of how HNH endonucleases enhance terminase-mediated digestion of the cos site and contribute to the phage replication cycle. IMPORTANCE This study demonstrates that residues outside the canonical ββα fold, namely, the Ω-loop α-helical insert and a disordered C-terminal tail, regulate the activity of the HNH endonuclease gp74. The increased divalent metal ion binding when the Ω-loop insert is removed compared to reduced cos site digestion and phage formation indicates that the Ω-loop insert plays multiple regulatory roles. The data presented here provide insights into the molecular basis of the involvement of HNH proteins in phage DNA packing.

scholarly journals Conformational Plasticity of the Adenylyl Cyclase CyaA from Bordetella Pertussis

2010 ◽  
Vol 98 (3) ◽  
pp. 58a
Author(s):  
Edithe Selwa ◽  
Elodie Laine ◽  
Thérèse E. Malliavin
Keyword(s):  
Adenylyl Cyclase ◽  
Bordetella Pertussis ◽  
Conformational Plasticity

scholarly journals Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin

2005 ◽  
Vol 24 (18) ◽  
pp. 3190-3201 ◽  
Author(s):  
Qing Guo ◽  
Yuequan Shen ◽  
Young-Sam Lee ◽  
Craig S Gibbs ◽  
Milan Mrksich ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Bordetella Pertussis ◽  
Structural Basis

scholarly journals Impact of divalent metal ions on regulation of adenylyl cyclase isoforms by forskolin analogs

2011 ◽  
Vol 82 (11) ◽  
pp. 1673-1681 ◽  
Author(s):  
Miriam Erdorf ◽  
Tung-Chung Mou ◽  
Roland Seifert
Keyword(s):  
Metal Ions ◽  
Adenylyl Cyclase ◽  
Divalent Metal ◽  
Divalent Metal Ions
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