The Effects of the Site-Directed Removal of N-Glycosylation from Cationic Peanut Peroxidase on Its Function

2001 ◽  
Vol 386 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Bao Lige ◽  
Shengwu Ma ◽  
Robert B. van Huystee
Keyword(s):  
Peanut Peroxidase

Cationic peanut peroxidase: expression and characterization in transgenic tobacco and purification of the histidine-tagged protein

Plant Science ◽  
1998 ◽  
Vol 136 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Bao Lige ◽  
Shengwu Ma ◽  
Dongling Zhao ◽  
Robert B. van Huystee
Keyword(s):  
Transgenic Tobacco ◽  
Peanut Peroxidase

HPLC Determination of the Sugar Compositions of the Glycans on the Cationic Peanut Peroxidase

1997 ◽  
Vol 45 (11) ◽  
pp. 4196-4200 ◽  
Author(s):  
Yan Sun ◽  
Bao Lige ◽  
Robert B. van Huystee
Keyword(s):  
Hplc Determination ◽  
Peanut Peroxidase

The heme moiety in peanut peroxidase

1984 ◽  
Vol 62 (11) ◽  
pp. 1046-1050 ◽  
Author(s):  
R. N. Chibbar ◽  
R. Cella ◽  
R. B. Van Huystee
Keyword(s):  
Peroxidase Activity ◽  
Active Site ◽  
Oxidase Activity ◽  
Indoleacetic Acid ◽  
Iaa Oxidase ◽  
Equimolar Ratio ◽  
Immunological Properties ◽  
Peanut Peroxidase

Heme is present in an equimolar ratio to the apoprotein in the major cationic fraction of peanut peroxidase. The removal of heme from the holoenzyme does not affect the physicochemical and immunological properties of the apoperoxidase, however peroxidase activity is completely lost. The indoleacetic acid (IAA) oxidase activity of the apoperoxidase is reduced to 1/20 of the original holoenzyme. Both the peroxidase and IAA-oxidase activity could partially be restored in the holoenzyme reconstituted with hemin. It is suggested that heme may also participate in the IAA-oxidase activity possibly by altering the active site.

Immunoaffinity Studies on Cationic Peanut Peroxidase Fraction

1984 ◽  
Vol 116 (4) ◽  
pp. 365-373 ◽  
Author(s):  
Ravindra N. Chibbar ◽  
Robert B. Van Huystee
Keyword(s):  
Peanut Peroxidase

Removal of the N-linked glycan structure from the peanut peroxidase prxPNC2: Influence on protein stability and activity

2005 ◽  
Vol 66 (16) ◽  
pp. 1869-1879 ◽  
Author(s):  
Ranjith Pathirana ◽  
Lyn Watson ◽  
Balance Chen ◽  
Susanna Leung ◽  
Christine Voisey ◽  
...  
Keyword(s):  
Protein Stability ◽  
Glycan Structure ◽  
Peanut Peroxidase

Spectral and molecular properties of peanut peroxidase isozymes

1977 ◽  
Vol 16 (11) ◽  
pp. 1657-1659 ◽  
Author(s):  
Om.P. Srivastava ◽  
R.B. van Huystee
Keyword(s):  
Molecular Properties ◽  
Peroxidase Isozymes ◽  
Peanut Peroxidase

scholarly journals Production and Preliminary Characterization of Monoclonal Antibodies against Cationic Peanut Peroxidase

1987 ◽  
Vol 85 (1) ◽  
pp. 299-303 ◽  
Author(s):  
Chunfang Hu ◽  
Daniela Carbonera ◽  
Robert van Huystee
Keyword(s):  
Monoclonal Antibodies ◽  
Preliminary Characterization ◽  
Peanut Peroxidase
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