Mechanistic Studies to Evaluate the Enhanced Antiproliferation of Human Keratinocytes by 1α,25-Dihydroxyvitamin D3–3-bromoacetate, a Covalent Modifier of Vitamin D Receptor, Compared to 1α,25-Dihydroxyvitamin D3

1999 ◽  
Vol 370 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Ming L. Chen ◽  
Swapna Ray ◽  
Narasimha Swamy ◽  
Michael F. Holick ◽  
Rahul Ray
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Human Keratinocytes ◽  
Mechanistic Studies ◽  
Dihydroxyvitamin D3

scholarly journals Hairless Suppresses Vitamin D Receptor Transactivation in Human Keratinocytes

Endocrinology ◽  
2006 ◽  
Vol 147 (1) ◽  
pp. 314-323 ◽  
Author(s):  
Zhongjian Xie ◽  
Sandra Chang ◽  
Yuko Oda ◽  
Daniel D. Bikle
Keyword(s):  
Vitamin D ◽  
Hair Follicle ◽  
Vitamin D Receptor ◽  
Human Keratinocytes ◽  
Potential Interaction ◽  
Keratinocyte Differentiation ◽  
Mobility Shift ◽  
Dihydroxyvitamin D3 ◽  
Interacting Protein ◽  
Hydroxyvitamin D

The vitamin D receptor (VDR) and its ligand 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] are required for normal keratinocyte differentiation. Both the epidermis and the hair follicle are disrupted in VDR-null mice. Hairless (Hr), a presumptive transcription factor with no known ligand, when mutated, disrupts hair follicle cycling similar to the effects of VDR mutations. Hr, like VDR, is found in the nuclei of keratinocytes in both epidermis and hair follicle. To investigate the potential interaction between Hr and VDR on keratinocyte differentiation, we examined the effect of Hr expression on vitamin D-responsive genes in normal human keratinocytes. Inhibition of Hr expression in keratinocytes potentiated the induction of vitamin D-responsive genes, including involucrin, transglutaminase, phospholipase C-γ1, and 25-hydroxyvitamin D-24-hydroxylase (24-hydroxylase) by 1,25(OH)2D3. Overexpression of Hr in human keratinocytes suppressed the induction of these vitamin D-responsive genes by 1,25(OH)2D3. Coimmunoprecipitation, DNA mobility shift assays, and chromatin immunoprecipitation revealed that Hr binds to VDR in human keratinocytes. Hr binding to the VDR was eliminated by 1,25(OH)2D3, which recruited the coactivator vitamin D receptor-interacting protein 205 (DRIP205) to the VDR/vitamin D response element complex. These data indicate that Hr functions as a corepressor of VDR to block 1,25(OH)2D3 action on keratinocytes.

Vitamin D receptor in IMR-90 human fibroblasts and antiproliferative effect of 1,25-dihydroxyvitamin D3

IUBMB Life ◽  
1997 ◽  
Vol 43 (6) ◽  
pp. 1173-1181 ◽  
Author(s):  
M. Stio ◽  
A. Celli ◽  
B. Lunghi ◽  
G. Raugei ◽  
A. Modesti ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Human Fibroblasts ◽  
Antiproliferative Effect ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

Expression of vitamin D receptor, CYP27B1 and CYP24A1 hydroxylases and 1,25-dihydroxyvitamin D3 levels in stone formers

Urolithiasis ◽  
2019 ◽  
Vol 48 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Thalita Lima Melo ◽  
Priscila Ligeiro Gonçalves Esper ◽  
Lysien Ivania Zambrano ◽  
Milene Subtil Ormanji ◽  
Fernanda Guedes Rodrigues ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Stone Formers

scholarly journals Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium

2003 ◽  
Vol 100 (17) ◽  
pp. 9733-9737 ◽  
Author(s):  
K. D. Healy ◽  
J. B. Zella ◽  
J. M. Prahl ◽  
H. F. DeLuca
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

scholarly journals SIRT1 enzymatically potentiates 1,25-dihydroxyvitamin D3 signaling via vitamin D receptor deacetylation

2017 ◽  
Vol 172 ◽  
pp. 117-129 ◽  
Author(s):  
Marya S. Sabir ◽  
Zainab Khan ◽  
Chengcheng Hu ◽  
Michael A. Galligan ◽  
Christopher M. Dussik ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3
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