Transcriptional Regulation of Cellular Retinol-Binding Protein, Type II Gene Expression in Small Intestine by Dietary Fat

1999 ◽  
Vol 362 (1) ◽  
pp. 159-166 ◽  
Author(s):  
Kazuhito Suruga ◽  
Kazuki Mochizuki ◽  
Masaaki Kitagawa ◽  
Toshinao Goda ◽  
Nobuyuki Horie ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Small Intestine ◽  
Dietary Fat ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Type Ii ◽  
Cellular Retinol Binding Protein

scholarly journals Diet-related variation in cellular retinol-binding protein type II gene expression in rat jejunum

2005 ◽  
Vol 94 (6) ◽  
pp. 890-895 ◽  
Author(s):  
Kazuhito Suruga ◽  
Masaaki Kitagawa ◽  
Hiromitsu Yasutake ◽  
Sachiko Takase ◽  
Toshinao Goda
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Binding Protein ◽  
Dietary Fatty Acids ◽  
Retinol Binding Protein ◽  
Rat Jejunum ◽  
Type Ii ◽  
Feeding Period ◽  
Related Variation ◽  
Cellular Retinol Binding Protein

Cellular retinol-binding protein type II (CRBPII) is involved in the transport of vitamin A and its metabolism in the small intestine. In the present study, we demonstrated diet-related variations in CRBPII expression in rat jejunum. The CRBPII protein and mRNA levels increased in parallel after the start of feeding period regardless of whether the feeding period was restricted to the hours of darkness or of light. In addition, this variation was observed in the rats fed high-fat diet or low-fat diets, but not in those fed a fat-free diet or in fasted rats. A similar diet-induced variation was seen in the mRNA of liver-type fatty acid-binding protein in rat jejunum. In the transient transfection experiment, unsaturated fatty acid increased rat CRBPII gene promoter activity via the PPARα/retinoid X receptor-α heterodimer. Taken together, these results suggest that the diet-related variation in CRBPII expression in rat jejunum may be brought about by the transcriptional induction of CRBPII gene expression mainly triggered by dietary fatty acids.

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